Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J3989_RS08710 Genome accession   NZ_AP024489
Coordinates   1774926..1776056 (-) Length   376 a.a.
NCBI ID   WP_002214012.1    Uniprot ID   X5EQV8
Organism   Neisseria meningitidis strain NIID777     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1769926..1781056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J3989_RS08670 (NMNIID777_16200) - 1770376..1770798 (-) 423 WP_002219529.1 protein Gly1ORF1 -
  J3989_RS08675 (NMNIID777_16210) - 1771049..1771369 (-) 321 WP_002214001.1 DUF2322 family protein -
  J3989_RS08680 (NMNIID777_16220) upp 1771392..1772018 (-) 627 WP_002219530.1 uracil phosphoribosyltransferase -
  J3989_RS08685 (NMNIID777_16230) grxD 1772124..1772435 (-) 312 WP_002214004.1 Grx4 family monothiol glutaredoxin -
  J3989_RS08690 (NMNIID777_16240) - 1772536..1772751 (-) 216 WP_002219531.1 DUF333 domain-containing protein -
  J3989_RS08695 (NMNIID777_16250) - 1772777..1773556 (-) 780 WP_002219532.1 TatD family hydrolase -
  J3989_RS08700 (NMNIID777_16260) - 1773561..1773911 (-) 351 WP_002214009.1 PilZ domain-containing protein -
  J3989_RS08705 (NMNIID777_16270) - 1773915..1774892 (-) 978 WP_002223913.1 DNA polymerase III subunit delta' -
  J3989_RS08710 (NMNIID777_16280) pilU 1774926..1776056 (-) 1131 WP_002214012.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J3989_RS08715 (NMNIID777_16290) - 1776224..1776925 (+) 702 WP_002219535.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  J3989_RS08720 (NMNIID777_16300) lepA 1777067..1778860 (+) 1794 WP_002219536.1 translation elongation factor 4 -
  J3989_RS08725 (NMNIID777_16310) lepB 1779003..1780022 (+) 1020 WP_061694891.1 signal peptidase I -
  J3989_RS08730 - 1780241..1780390 (-) 150 WP_003708054.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.36 Da        Isoelectric Point: 6.4333

>NTDB_id=85363 J3989_RS08710 WP_002214012.1 1774926..1776056(-) (pilU) [Neisseria meningitidis strain NIID777]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=85363 J3989_RS08710 WP_002214012.1 1774926..1776056(-) (pilU) [Neisseria meningitidis strain NIID777]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCAATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
TGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGATTACCGCAATGAAAAT
TCGTTCGGACATATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTCATCCTTATCGGCG
AAATCCGCGACCGCGAAACAATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGATCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB X5EQV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.768

87.234

0.364

Multiple sequence alignment