Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JUNP479_RS20590 Genome accession   NZ_AP024466
Coordinates   4430410..4431549 (-) Length   379 a.a.
NCBI ID   WP_041208567.1    Uniprot ID   -
Organism   Aeromonas jandaei strain JUNP479     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4425410..4436549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JUNP479_RS20570 (JUNP479_4181) oppF 4426194..4427189 (+) 996 WP_033114332.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -
  JUNP479_RS20575 (JUNP479_4182) - 4427491..4428435 (+) 945 WP_041208571.1 TerC family protein -
  JUNP479_RS20580 (JUNP479_4183) alr 4428492..4429718 (-) 1227 WP_043849498.1 alanine racemase -
  JUNP479_RS20585 (JUNP479_4184) - 4429894..4430343 (+) 450 WP_203975682.1 methyltransferase family protein -
  JUNP479_RS20590 (JUNP479_4185) pilU 4430410..4431549 (-) 1140 WP_041208567.1 type IVa pilus ATPase TapW Machinery gene
  JUNP479_RS20595 (JUNP479_4186) - 4431609..4431965 (-) 357 WP_005302630.1 DMT family protein -
  JUNP479_RS20600 (JUNP479_4187) - 4432078..4432812 (+) 735 WP_203975684.1 substrate-binding periplasmic protein -
  JUNP479_RS20605 (JUNP479_4188) btuC 4432915..4433922 (+) 1008 WP_203975686.1 vitamin B12 ABC transporter permease BtuC -
  JUNP479_RS20610 (JUNP479_4189) - 4433909..4434694 (+) 786 WP_203975687.1 ATP-binding cassette domain-containing protein -
  JUNP479_RS20615 (JUNP479_4190) - 4435104..4436168 (-) 1065 WP_041208564.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42273.33 Da        Isoelectric Point: 6.6722

>NTDB_id=85255 JUNP479_RS20590 WP_041208567.1 4430410..4431549(-) (pilU) [Aeromonas jandaei strain JUNP479]
MELRDMLQTLAKQDGSDLYLSTGAPPCAKFNGVLRPLNETPLNPGDVAAIAEAIMDKEQKEQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKGDHF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=85255 JUNP479_RS20590 WP_041208567.1 4430410..4431549(-) (pilU) [Aeromonas jandaei strain JUNP479]
ATGGAATTGCGAGACATGTTACAGACCCTGGCCAAACAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCCTG
CGCCAAATTCAACGGGGTACTGCGCCCCCTGAATGAGACACCGCTCAACCCGGGCGATGTGGCGGCCATTGCCGAGGCCA
TCATGGACAAGGAGCAGAAGGAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCGCAGATCGGCCGT
TTCCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTAGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTACTGCTCGACACCATCATGGAGAAGCGCGGCTTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGTAAATCGACCTCGCTGGCGGCGCTCATCGATCATCGCAACCGCAATAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTTCATCGCCACCGCAAGAGCATCATCAATCAGCGGGAAGTGGGCGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATCCTGATCGGGGAAATTCGTGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGCCCGCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAGACTACCGATGGCGGTCGCCGGGCCGCAGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAAGCGCTCGGCATGGTCACTTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTCATAGATGAAGAGGAGGCAGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTATGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGTCTCGAACCAACCAAGGACG
AGAAGGGCGACCATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.211

100

0.544
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

50.857

92.348

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.36

93.14

0.385
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.988

90.765

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361

Multiple sequence alignment