Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JSX26_RS08375 Genome accession   NZ_AP024402
Coordinates   1746766..1747902 (-) Length   378 a.a.
NCBI ID   WP_029314740.1    Uniprot ID   -
Organism   Aeromonas caviae strain KAM376     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1741766..1752902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JSX26_RS08350 (KAM376_16320) - 1742661..1743218 (-) 558 WP_010675206.1 TMEM165/GDT1 family protein -
  JSX26_RS08355 (KAM376_16330) - 1743472..1743801 (-) 330 WP_039041208.1 translation initiation factor -
  JSX26_RS08360 (KAM376_16340) - 1743921..1744148 (+) 228 WP_010675204.1 FeoA family protein -
  JSX26_RS08365 (KAM376_16350) feoB 1744145..1746415 (+) 2271 WP_203764826.1 Fe(2+) transporter permease subunit FeoB -
  JSX26_RS08370 (KAM376_16360) - 1746461..1746703 (+) 243 WP_039041210.1 FeoC-like transcriptional regulator -
  JSX26_RS08375 (KAM376_16370) pilU 1746766..1747902 (-) 1137 WP_029314740.1 type IVa pilus ATPase TapW Machinery gene
  JSX26_RS08380 (KAM376_16380) - 1747959..1748315 (-) 357 WP_010675200.1 DMT family protein -
  JSX26_RS08385 (KAM376_16390) - 1748428..1749159 (+) 732 WP_203764828.1 substrate-binding periplasmic protein -
  JSX26_RS08390 (KAM376_16400) btuC 1749235..1750242 (+) 1008 WP_039041213.1 vitamin B12 ABC transporter permease BtuC -
  JSX26_RS08395 (KAM376_16410) - 1750229..1751002 (+) 774 WP_203765182.1 ATP-binding cassette domain-containing protein -
  JSX26_RS08400 (KAM376_16420) - 1751210..1752274 (-) 1065 WP_042014687.1 3-deoxy-7-phosphoheptulonate synthase -
  JSX26_RS08405 (KAM376_16430) - 1752416..1752796 (+) 381 WP_039040385.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42203.27 Da        Isoelectric Point: 6.6000

>NTDB_id=85043 JSX26_RS08375 WP_029314740.1 1746766..1747902(-) (pilU) [Aeromonas caviae strain KAM376]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLGPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=85043 JSX26_RS08375 WP_029314740.1 1746766..1747902(-) (pilU) [Aeromonas caviae strain KAM376]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
TGCCAAGTTCAACGGCGCCCTGCGCCCCCTCACCCAGGAGCCCCTGGGGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCTCCGGTGCTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAGTCCACGTCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGCGAAATCCGCGATCGGGAGACCATGGAGCATG
CCCTCGCCTTCTCGGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGACGCCGGGCGGCGGTGGAGGTGATGCTCGGAACCCACACTGTGCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGTACCCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCAGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGCCAGATTGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGTGACCTCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.514

94.709

0.497
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

89.683

0.381
  pilT Vibrio cholerae strain A1552

40.456

92.857

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.857

0.376
  pilT Legionella pneumophila strain ERS1305867

42.216

88.36

0.373
  pilT Legionella pneumophila strain Lp02

42.216

88.36

0.373
  pilT Pseudomonas aeruginosa PAK

40.988

91.005

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.317

88.36

0.365
  pilT Acinetobacter nosocomialis M2

41.317

88.36

0.365
  pilT Acinetobacter baumannii D1279779

41.317

88.36

0.365

Multiple sequence alignment