Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LL303_RS12290 Genome accession   NZ_CP126467
Coordinates   2386354..2387292 (-) Length   312 a.a.
NCBI ID   WP_031561106.1    Uniprot ID   A0AAP8DEI2
Organism   Lactococcus lactis subsp. lactis strain 303     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2381354..2392292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL303_RS12250 (LL303_12220) - 2382242..2383084 (-) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  LL303_RS12255 (LL303_12225) - 2383081..2383518 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  LL303_RS12260 (LL303_12230) comGG 2383599..2383883 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  LL303_RS12265 (LL303_12235) comGF 2383922..2384368 (-) 447 WP_031558968.1 competence type IV pilus minor pilin ComGF Machinery gene
  LL303_RS12270 (LL303_12240) comGE 2384331..2384627 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  LL303_RS12275 (LL303_12245) comGD 2384599..2384997 (-) 399 WP_023164614.1 competence type IV pilus minor pilin ComGD Machinery gene
  LL303_RS12280 (LL303_12250) comGC 2384990..2385373 (-) 384 WP_010906318.1 competence type IV pilus major pilin ComGC Machinery gene
  LL303_RS12285 (LL303_12255) comGB 2385387..2386460 (-) 1074 WP_095348492.1 competence type IV pilus assembly protein ComGB Machinery gene
  LL303_RS12290 (LL303_12260) comGA 2386354..2387292 (-) 939 WP_031561106.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35707.88 Da        Isoelectric Point: 5.9473

>NTDB_id=838052 LL303_RS12290 WP_031561106.1 2386354..2387292(-) (comGA) [Lactococcus lactis subsp. lactis strain 303]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=838052 LL303_RS12290 WP_031561106.1 2386354..2387292(-) (comGA) [Lactococcus lactis subsp. lactis strain 303]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.923

100

0.769
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519