Detailed information NTG0838047

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LL303_RS12265
• Genome accession: NZ_CP126467
• Coordinates: 2383922..2384368 (-)
• Length: 148 a.a.
• NCBI ID: WP_031558968.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain 303
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2378922..2389368

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL303_RS12230 (LL303_12200)	-	2379039..2379416 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
LL303_RS12235 (LL303_12205)	-	2379621..2380487 (+)	867	WP_010906309.1	RluA family pseudouridine synthase	-
LL303_RS12240 (LL303_12210)	-	2380526..2381335 (-)	810	WP_010906310.1	metal ABC transporter permease	-
LL303_RS12245 (LL303_12215)	-	2381328..2382065 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
LL303_RS12250 (LL303_12220)	-	2382242..2383084 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
LL303_RS12255 (LL303_12225)	-	2383081..2383518 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
LL303_RS12260 (LL303_12230)	comGG	2383599..2383883 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
LL303_RS12265 (LL303_12235)	comGF	2383922..2384368 (-)	447	WP_031558968.1	competence type IV pilus minor pilin ComGF	Machinery gene
LL303_RS12270 (LL303_12240)	comGE	2384331..2384627 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LL303_RS12275 (LL303_12245)	comGD	2384599..2384997 (-)	399	WP_023164614.1	competence type IV pilus minor pilin ComGD	Machinery gene
LL303_RS12280 (LL303_12250)	comGC	2384990..2385373 (-)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
LL303_RS12285 (LL303_12255)	comGB	2385387..2386460 (-)	1074	WP_095348492.1	competence type IV pilus assembly protein ComGB	Machinery gene
LL303_RS12290 (LL303_12260)	comGA	2386354..2387292 (-)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17069.78 Da
• Isoelectric Point: 8.2389

>NTDB_id=838047 LL303_RS12265 WP_031558968.1 2383922..2384368(-) (comGF) [Lactococcus lactis subsp. lactis strain 303]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSINDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=838047 LL303_RS12265 WP_031558968.1 2383922..2384368(-) (comGF) [Lactococcus lactis subsp. lactis strain 303]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
ATGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	74.82	93.919	0.703
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	42.336	92.568	0.392
comGF/cglF	Streptococcus mitis NCTC 12261	40.876	92.568	0.378
comGF/cglF	Streptococcus pneumoniae Rx1	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae D39	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae R6	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae TIGR4	39.716	95.27	0.378