Detailed information NTG0827523

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: QKA52_RS25350
• Genome accession: NZ_CP124667
• Coordinates: 5401319..5401771 (-)
• Length: 150 a.a.
• NCBI ID: WP_282414023.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 2021CK-01315
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5396319..5406771

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QKA52_RS25320 (QKA52_25320)	-	5396592..5396813 (+)	222	WP_044061914.1	hypothetical protein	-
QKA52_RS25325 (QKA52_25325)	-	5396892..5397116 (+)	225	WP_033869155.1	hypothetical protein	-
QKA52_RS25330 (QKA52_25330)	ampD	5397209..5397580 (-)	372	Protein_5009	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
QKA52_RS25335 (QKA52_25335)	-	5397731..5399986 (-)	2256	WP_003104913.1	DUF1631 domain-containing protein	-
QKA52_RS25340 (QKA52_25340)	nadC	5400272..5401120 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
QKA52_RS25350 (QKA52_25350)	pilA	5401319..5401771 (-)	453	WP_282414023.1	pilin	Machinery gene
QKA52_RS25355 (QKA52_25355)	pilB	5402003..5403703 (+)	1701	WP_003112841.1	type IV-A pilus assembly ATPase PilB	Machinery gene
QKA52_RS25360 (QKA52_25360)	pilC	5403707..5404927 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
QKA52_RS25365 (QKA52_25365)	pilD	5404931..5405803 (+)	873	WP_003112839.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
QKA52_RS25370 (QKA52_25370)	coaE	5405800..5406411 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
QKA52_RS25375 (QKA52_25375)	yacG	5406408..5406608 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 15702.02 Da
• Isoelectric Point: 7.7813

>NTDB_id=827523 QKA52_RS25350 WP_282414023.1 5401319..5401771(-) (pilA) [Pseudomonas aeruginosa strain 2021CK-01315]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGITGSKILIGTTASTADTTYV
GIDEKANKLGTVAVDIKDTGDGTVKFTFATGQSSPKNAGTAITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide

• Length: 453 bp

>NTDB_id=827523 QKA52_RS25350 WP_282414023.1 5401319..5401771(-) (pilA) [Pseudomonas aeruginosa strain 2021CK-01315]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACTACTGTTG
AAGAGTCGCTGTCGCGTGGAATTACTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGATATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGCAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

• Pilin (bacterial filament) (Pilin) (35-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	65.789	100	0.667
pilA/pilAI	Pseudomonas stutzeri DSM 10701	46.667	100	0.467
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.5	100	0.453
pilA	Vibrio cholerae strain A1552	42.5	100	0.453
pilA	Vibrio cholerae C6706	42.5	100	0.453
pilA	Acinetobacter baumannii strain A118	45.27	98.667	0.447
pilA	Vibrio parahaemolyticus RIMD 2210633	42.568	98.667	0.42
comP	Acinetobacter baylyi ADP1	41.333	100	0.413
pilA/pilAII	Pseudomonas stutzeri DSM 10701	42.657	95.333	0.407
pilA2	Legionella pneumophila strain ERS1305867	39.597	99.333	0.393
pilA2	Legionella pneumophila str. Paris	38.926	99.333	0.387
pilA	Acinetobacter nosocomialis M2	42.537	89.333	0.38
pilA	Ralstonia pseudosolanacearum GMI1000	39.161	95.333	0.373