Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   P7I85_RS24875 Genome accession   NZ_CP124622
Coordinates   5331786..5332238 (-) Length   150 a.a.
NCBI ID   WP_282414023.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2021CK-01159     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5326786..5337238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I85_RS24855 (P7I85_24855) ampD 5327481..5328047 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  P7I85_RS24860 (P7I85_24860) - 5328198..5330453 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  P7I85_RS24865 (P7I85_24865) nadC 5330739..5331587 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  P7I85_RS24875 (P7I85_24875) pilA 5331786..5332238 (-) 453 WP_282414023.1 pilin Machinery gene
  P7I85_RS24880 (P7I85_24880) pilB 5332470..5334170 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P7I85_RS24885 (P7I85_24885) pilC 5334174..5335394 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  P7I85_RS24890 (P7I85_24890) pilD 5335398..5336270 (+) 873 WP_003112839.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  P7I85_RS24895 (P7I85_24895) coaE 5336267..5336878 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  P7I85_RS24900 (P7I85_24900) yacG 5336875..5337075 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15702.02 Da        Isoelectric Point: 7.7813

>NTDB_id=826819 P7I85_RS24875 WP_282414023.1 5331786..5332238(-) (pilA) [Pseudomonas aeruginosa strain 2021CK-01159]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGITGSKILIGTTASTADTTYV
GIDEKANKLGTVAVDIKDTGDGTVKFTFATGQSSPKNAGTAITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=826819 P7I85_RS24875 WP_282414023.1 5331786..5332238(-) (pilA) [Pseudomonas aeruginosa strain 2021CK-01159]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACTACTGTTG
AAGAGTCGCTGTCGCGTGGAATTACTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGATATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGCAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

65.789

100

0.667
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.667

100

0.467
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.5

100

0.453
  pilA Vibrio cholerae strain A1552

42.5

100

0.453
  pilA Vibrio cholerae C6706

42.5

100

0.453
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.657

95.333

0.407
  pilA2 Legionella pneumophila strain ERS1305867

39.597

99.333

0.393
  pilA2 Legionella pneumophila str. Paris

38.926

99.333

0.387
  pilA Acinetobacter nosocomialis M2

42.537

89.333

0.38
  pilA Ralstonia pseudosolanacearum GMI1000

39.161

95.333

0.373