Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   QG303_RS04770 Genome accession   NZ_CP123909
Coordinates   1041425..1041847 (-) Length   140 a.a.
NCBI ID   WP_122610922.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain MGMM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1036425..1046847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QG303_RS04755 (QG303_04755) - 1037270..1040221 (+) 2952 WP_160768587.1 DUF748 domain-containing protein -
  QG303_RS04760 (QG303_04760) - 1040218..1040517 (+) 300 WP_024014339.1 DUF2845 domain-containing protein -
  QG303_RS04765 (QG303_04765) - 1040777..1041130 (-) 354 WP_016773155.1 BON domain-containing protein -
  QG303_RS04770 (QG303_04770) pilA/pilAI 1041425..1041847 (-) 423 WP_122610922.1 pilin Machinery gene
  QG303_RS04775 (QG303_04775) pilB 1042074..1043774 (+) 1701 WP_258603690.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QG303_RS04780 (QG303_04780) pilC 1043777..1044994 (+) 1218 WP_095181835.1 type II secretion system F family protein Machinery gene
  QG303_RS04785 (QG303_04785) pilD 1044996..1045868 (+) 873 WP_281120865.1 A24 family peptidase Machinery gene
  QG303_RS04790 (QG303_04790) coaE 1045865..1046488 (+) 624 WP_281120866.1 dephospho-CoA kinase -
  QG303_RS04795 (QG303_04795) yacG 1046485..1046685 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14130.29 Da        Isoelectric Point: 8.4918

>NTDB_id=823026 QG303_RS04770 WP_122610922.1 1041425..1041847(-) (pilA/pilAI) [Pseudomonas atacamensis strain MGMM4]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDLINQGTNPTLAAVKGDNVTTTSNCA
LTASGTAADGAGSIVCTLANAPAPVLGKTITLSRTAAGGWSCGTTVAPDYSPKGCNATGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=823026 QG303_RS04770 WP_122610922.1 1041425..1041847(-) (pilA/pilAI) [Pseudomonas atacamensis strain MGMM4]
ATGAAAAAACAACAAGGTTTCACATTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCGACTATCGCCCT
GCCTCAGTATTCGAAGTATCAGGCGCGGTCGAAGGTGACGGCGGGGTTGGCGGAGATCAGCGCGATGAAGGTGCCGTTTG
AAGACCTGATCAATCAGGGGACAAATCCAACCTTGGCGGCAGTGAAAGGCGATAACGTCACCACGACCTCAAATTGCGCT
CTGACTGCCTCAGGTACCGCTGCGGATGGAGCAGGATCGATTGTATGTACGCTGGCCAACGCGCCGGCACCTGTTTTAGG
GAAAACCATCACGCTGTCTCGTACGGCGGCCGGCGGTTGGAGTTGTGGCACTACGGTCGCTCCTGACTACTCGCCAAAAG
GCTGTAACGCTACTGGTACTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

49.635

97.857

0.486
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.308

100

0.471
  pilA Vibrio cholerae strain A1552

42.308

100

0.471
  pilA Vibrio cholerae C6706

42.308

100

0.471
  pilA Acinetobacter baumannii strain A118

46.763

99.286

0.464
  comP Acinetobacter baylyi ADP1

41.176

100

0.45
  pilA Pseudomonas aeruginosa PAK

39.735

100

0.429
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA Vibrio parahaemolyticus RIMD 2210633

40.741

96.429

0.393
  pilA2 Legionella pneumophila str. Paris

37.671

100

0.393
  pilA2 Legionella pneumophila strain ERS1305867

35.616

100

0.371