Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PY254_RS06650 Genome accession   NZ_CP119392
Coordinates   1565055..1565489 (-) Length   144 a.a.
NCBI ID   WP_281014691.1    Uniprot ID   -
Organism   Rhodanobacter sp. AS-Z3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1560055..1570489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY254_RS06640 (PY254_06640) - 1560618..1562735 (-) 2118 WP_281015173.1 hypothetical protein -
  PY254_RS06645 (PY254_06645) - 1563021..1564775 (-) 1755 WP_281014690.1 hypothetical protein -
  PY254_RS06650 (PY254_06650) pilA/pilAI 1565055..1565489 (-) 435 WP_281014691.1 pilin Machinery gene
  PY254_RS06655 (PY254_06655) - 1565922..1566350 (-) 429 WP_281014692.1 pilin -
  PY254_RS06660 (PY254_06660) - 1566476..1568536 (-) 2061 WP_281014693.1 acyltransferase family protein -
  PY254_RS06665 (PY254_06665) - 1568742..1569152 (-) 411 WP_281014694.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
  PY254_RS06670 (PY254_06670) - 1569132..1569346 (+) 215 Protein_1316 type II toxin-antitoxin system RelE/ParE family toxin -
  PY254_RS06675 (PY254_06675) - 1569428..1569709 (+) 282 WP_281015174.1 HigA family addiction module antitoxin -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15084.32 Da        Isoelectric Point: 8.8610

>NTDB_id=798793 PY254_RS06650 WP_281014691.1 1565055..1565489(-) (pilA/pilAI) [Rhodanobacter sp. AS-Z3]
MKTMQKGFTLIELMIVVAIIAILAAIALPQYQNYVARAQATAGLADITPGKTAFEEQINNGIQTTASYVATQIGLQDTTE
RCAVTSTLTSFTNGTGTIACTLKGNPKVAGVALTWTRNSSGTWACSNSGDATFKAKFAPKGCNL

Nucleotide


Download         Length: 435 bp        

>NTDB_id=798793 PY254_RS06650 WP_281014691.1 1565055..1565489(-) (pilA/pilAI) [Rhodanobacter sp. AS-Z3]
ATGAAGACGATGCAGAAAGGTTTTACCCTGATCGAATTGATGATCGTTGTTGCGATCATCGCGATCCTGGCCGCCATTGC
GCTGCCGCAATACCAGAACTACGTGGCACGTGCTCAGGCCACCGCCGGCTTGGCTGACATCACTCCGGGAAAGACCGCAT
TTGAGGAACAGATCAACAACGGCATCCAGACCACCGCGTCGTACGTCGCAACCCAGATTGGCCTTCAGGACACCACCGAA
CGCTGCGCCGTTACTAGTACTTTGACTAGTTTCACCAACGGCACGGGCACGATCGCATGCACTCTGAAGGGTAACCCCAA
GGTCGCTGGCGTTGCCCTGACTTGGACGCGCAACAGCAGCGGCACGTGGGCTTGCAGCAACAGCGGCGACGCAACCTTCA
AGGCCAAGTTCGCACCGAAGGGTTGCAACTTGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.966

100

0.493
  pilA Pseudomonas aeruginosa PAK

42.308

100

0.458
  pilA Acinetobacter baumannii strain A118

46.429

97.222

0.451
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.043

96.528

0.444
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.216

100

0.424
  pilA Vibrio cholerae C6706

41.216

100

0.424
  pilA Vibrio cholerae strain A1552

41.216

100

0.424
  pilA Ralstonia pseudosolanacearum GMI1000

36.364

100

0.417
  comP Acinetobacter baylyi ADP1

39.189

100

0.403
  pilA2 Legionella pneumophila str. Paris

38.776

100

0.396
  pilA2 Legionella pneumophila strain ERS1305867

38.776

100

0.396
  pilA Haemophilus influenzae Rd KW20

37.931

100

0.382
  pilA Vibrio parahaemolyticus RIMD 2210633

42.063

87.5

0.368