Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PYU98_RS14625 Genome accession   NZ_CP118988
Coordinates   3163842..3164981 (+) Length   379 a.a.
NCBI ID   WP_275056492.1    Uniprot ID   A0AAX3NSR6
Organism   Aeromonas allosaccharophila strain K520     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3158842..3169981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYU98_RS14600 (PYU98_14600) - 3159306..3160373 (+) 1068 WP_275056488.1 3-deoxy-7-phosphoheptulonate synthase -
  PYU98_RS14605 (PYU98_14605) - 3160612..3161409 (-) 798 WP_275056489.1 ATP-binding cassette domain-containing protein -
  PYU98_RS14610 (PYU98_14610) btuC 3161475..3162482 (-) 1008 WP_275056490.1 vitamin B12 ABC transporter permease BtuC -
  PYU98_RS14615 (PYU98_14615) - 3162582..3163313 (-) 732 WP_275056491.1 transporter substrate-binding domain-containing protein -
  PYU98_RS14620 (PYU98_14620) - 3163427..3163783 (+) 357 WP_075112061.1 DMT family protein -
  PYU98_RS14625 (PYU98_14625) pilU 3163842..3164981 (+) 1140 WP_275056492.1 type IVa pilus ATPase TapW Machinery gene
  PYU98_RS14630 (PYU98_14630) - 3165078..3165527 (-) 450 WP_275056493.1 isoprenylcysteine carboxylmethyltransferase family protein -
  PYU98_RS14635 (PYU98_14635) alr 3165701..3166927 (+) 1227 WP_075112059.1 alanine racemase -
  PYU98_RS14640 (PYU98_14640) - 3167124..3168068 (-) 945 WP_275056494.1 TerC family protein -
  PYU98_RS14645 (PYU98_14645) oppF 3168371..3169366 (-) 996 WP_275056495.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42280.42 Da        Isoelectric Point: 6.4293

>NTDB_id=797177 PYU98_RS14625 WP_275056492.1 3163842..3164981(+) (pilU) [Aeromonas allosaccharophila strain K520]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKLQFDKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHAIAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDMF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=797177 PYU98_RS14625 WP_275056492.1 3163842..3164981(+) (pilU) [Aeromonas allosaccharophila strain K520]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGGCCTGCGCCCCCTGAATGAAACGCCGCTGGAGCCGGGAGAGGTGGCACGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAACTGCAGTTTGACAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACTGAAATCCCCCGCTTTGA
AGACCTGAAACTTCCGCCGGTGCTGCTCGACACCATCATGGAAAAACGGGGGCTGGTGCTGTTTGTCGGCGGCACCGGCT
CGGGTAAATCGACCTCGCTGGCGGCGTTGATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATTGAAGAT
CCGGTGGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTCGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTGATCCTCATCGGCGAAATTCGCGATCGCGAAACCATGGAACATG
CGATCGCCTTCTCGGAGACCGGCCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGACGGCCCCAACTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGCCGCCGGGCGGCGGTGGAGATCATGCTCGGCACCCACACCATCCGCGACATGATCA
AGCGCGGTGAATTTGGCGGCCTGAAAGAGGTGATGGAAAAATCCAAGGCGCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGTGTGATCGAGGAGGAGGAAGCAGTGAAGAACGCAGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAAGAGAAGGGACAGATTGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGACGATATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.211

100

0.544
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

89.446

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361
  pilT Vibrio cholerae strain A1552

41.141

87.863

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

87.863

0.361