Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PUN47_RS01880 Genome accession   NZ_CP118599
Coordinates   385875..386981 (+) Length   368 a.a.
NCBI ID   WP_202652348.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 10M-VF     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 380875..391981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN47_RS01845 (PUN47_01845) - 381225..381824 (-) 600 WP_032080473.1 XTP/dITP diphosphatase -
  PUN47_RS01850 (PUN47_01850) - 381848..382279 (-) 432 WP_032080472.1 DUF4426 domain-containing protein -
  PUN47_RS01855 (PUN47_01855) yggU 382339..382629 (-) 291 WP_024374559.1 DUF167 family protein YggU -
  PUN47_RS01860 (PUN47_01860) - 382629..383186 (-) 558 WP_024374560.1 YggT family protein -
  PUN47_RS01865 (PUN47_01865) proC 383238..384056 (-) 819 WP_202652343.1 pyrroline-5-carboxylate reductase -
  PUN47_RS01870 (PUN47_01870) - 384093..384800 (-) 708 WP_202652345.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUN47_RS01875 (PUN47_01875) pilT 384826..385863 (+) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  PUN47_RS01880 (PUN47_01880) pilU 385875..386981 (+) 1107 WP_202652348.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PUN47_RS01885 (PUN47_01885) ruvX 387047..387472 (-) 426 WP_024374565.1 Holliday junction resolvase RuvX -
  PUN47_RS01890 (PUN47_01890) - 387534..388097 (-) 564 WP_032080468.1 YqgE/AlgH family protein -
  PUN47_RS01895 (PUN47_01895) gshB 388137..389093 (-) 957 WP_024374566.1 glutathione synthase -
  PUN47_RS01900 (PUN47_01900) rsmE 389106..389837 (-) 732 WP_274889021.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PUN47_RS01905 (PUN47_01905) - 389934..390635 (-) 702 WP_202652354.1 endonuclease -
  PUN47_RS01910 (PUN47_01910) - 390754..391254 (-) 501 WP_202646486.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41218.19 Da        Isoelectric Point: 6.1380

>NTDB_id=793834 PUN47_RS01880 WP_202652348.1 385875..386981(+) (pilU) [Vibrio fluvialis strain 10M-VF]
MQLEECLQGMMTYKASDLYITVGAPVLYRVDGELRAQGEKLDEAQVSALLAAMMDEARHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPAVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGDLHELKSTMAKSKEVGMQTFDQALY
RLVVENKISEQDALHCADSANDLRLMLKTQRGDVTSSGSLDNVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=793834 PUN47_RS01880 WP_202652348.1 385875..386981(+) (pilU) [Vibrio fluvialis strain 10M-VF]
ATGCAACTCGAGGAGTGCCTGCAAGGCATGATGACGTATAAAGCGTCAGATCTATATATTACTGTGGGCGCCCCCGTGCT
GTACCGGGTCGATGGTGAACTGCGCGCGCAGGGGGAGAAGCTGGATGAAGCGCAGGTCAGCGCGCTGCTGGCTGCGATGA
TGGATGAAGCGCGCCATGCGGACTATCTGGCGACGCGTGAAGCTAACTTTGCCATCGTACGTGATTTGGGGCGTTTTCGC
GTCAGCGCTTTTTTTCAGCGCGAGCTACCTGGTGCGGTGATTCGCCGCATCGAAACGCAGATTCCGACCTTTGATGAACT
CAAACTGCCAGCAGTGCTGAAAGATCTCTCGATTGCCAAACGCGGTTTAGTGCTGGTGGTGGGGGCGACAGGATCGGGCA
AATCGACCACCATGGCCGCGATGACTGGCTATCGCAATACACATCGCAGCGGACATATTCTCACCGTCGAAGATCCGATT
GAATTTGTCCATGAGCACCAGCGTTGTATCGTGACGCAGCGCGAAGTGGGATTGGACACGGAAAGCTATGAAGTGGCGCT
GAAGAACTCGCTACGCCAGGCGCCGGATATGATTTTGATCGGTGAGATCCGCAGCCGTGAAACCATGGAATACGCAATGA
CCTTCGCCGAAACCGGTCACTTGTGCATGGCAACCCTGCACGCCAATAACGCCAACCAAGCGCTTGAGCGTATTTTGCAT
CTGGTGCCGAAAGAGCAGCGTGAGCAATTTCTGTTTGATCTGTCGATGAACCTCAAAGGCGTTGTTGGTCAGCAACTGAT
CCGCGATAAAAACGGCCAGGGGCGCCACGGCGTGTTTGAAGTACTGCTCAACAGCCCGCGGGTGTCTGACATTATTCGCC
GTGGCGATTTGCATGAGCTGAAAAGCACCATGGCGAAATCGAAAGAGGTTGGCATGCAAACCTTTGATCAGGCGTTGTAC
CGATTGGTGGTCGAGAACAAGATCAGCGAGCAGGATGCGCTGCACTGTGCCGATTCGGCCAACGATCTGCGTTTGATGCT
CAAGACTCAGCGGGGCGATGTGACCAGTAGCGGTTCGTTGGATAACGTGCAAATAGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.326

100

0.853
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

54.131

95.38

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44

88.315

0.389
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Acinetobacter baylyi ADP1

41.349

92.663

0.383
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas aeruginosa PAK

41.194

91.033

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.701

91.033

0.361