Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PUN47_RS01875 Genome accession   NZ_CP118599
Coordinates   384826..385863 (+) Length   345 a.a.
NCBI ID   WP_024374563.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 10M-VF     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 379826..390863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN47_RS01840 (PUN47_01840) hemW 380053..381225 (-) 1173 WP_202652356.1 radical SAM family heme chaperone HemW -
  PUN47_RS01845 (PUN47_01845) - 381225..381824 (-) 600 WP_032080473.1 XTP/dITP diphosphatase -
  PUN47_RS01850 (PUN47_01850) - 381848..382279 (-) 432 WP_032080472.1 DUF4426 domain-containing protein -
  PUN47_RS01855 (PUN47_01855) yggU 382339..382629 (-) 291 WP_024374559.1 DUF167 family protein YggU -
  PUN47_RS01860 (PUN47_01860) - 382629..383186 (-) 558 WP_024374560.1 YggT family protein -
  PUN47_RS01865 (PUN47_01865) proC 383238..384056 (-) 819 WP_202652343.1 pyrroline-5-carboxylate reductase -
  PUN47_RS01870 (PUN47_01870) - 384093..384800 (-) 708 WP_202652345.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUN47_RS01875 (PUN47_01875) pilT 384826..385863 (+) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  PUN47_RS01880 (PUN47_01880) pilU 385875..386981 (+) 1107 WP_202652348.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PUN47_RS01885 (PUN47_01885) ruvX 387047..387472 (-) 426 WP_024374565.1 Holliday junction resolvase RuvX -
  PUN47_RS01890 (PUN47_01890) - 387534..388097 (-) 564 WP_032080468.1 YqgE/AlgH family protein -
  PUN47_RS01895 (PUN47_01895) gshB 388137..389093 (-) 957 WP_024374566.1 glutathione synthase -
  PUN47_RS01900 (PUN47_01900) rsmE 389106..389837 (-) 732 WP_274889021.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PUN47_RS01905 (PUN47_01905) - 389934..390635 (-) 702 WP_202652354.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38227.83 Da        Isoelectric Point: 6.5038

>NTDB_id=793833 PUN47_RS01875 WP_024374563.1 384826..385863(+) (pilT) [Vibrio fluvialis strain 10M-VF]
MDISELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLIFEIMNDAQRSEYEEKLEVDFSFALPDIGR
FRVNAFHQSRGASAVFRTIPTVIPTLDQLEAPEIFHKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINATHNKHVLTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRLGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGASHGMQTMEQHA
KQLIAQGLVDPAEVQKKIELEMSAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=793833 PUN47_RS01875 WP_024374563.1 384826..385863(+) (pilT) [Vibrio fluvialis strain 10M-VF]
ATGGATATCTCTGAGTTACTGGAATTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCGGGTGTTCCTCCTAT
GGTACGCATCGATGGTGAAGTGAGAAAGCTTGGCGTACCTGCGTTTACACACGCTGATGTGCATCGATTAATTTTTGAAA
TTATGAATGATGCCCAGCGCAGTGAGTACGAAGAAAAGCTGGAAGTGGACTTTTCCTTTGCGTTACCGGACATTGGCCGA
TTTCGTGTCAACGCTTTCCATCAGTCGCGCGGCGCTTCTGCGGTATTTCGTACCATTCCGACCGTGATTCCGACTCTAGA
CCAGCTTGAGGCTCCGGAAATTTTCCACAAAATCGCCAATTACGAAAAAGGTTTGGTGCTGGTGACCGGACCGACCGGCT
CGGGTAAATCAACCACGTTAGCGGCGATGGTCGATTACATCAACGCAACCCATAACAAGCATGTGCTGACTATCGAAGAT
CCGATTGAGTTTGTGCATAGCAACAACAAATGCTTGATCAACCAGCGTGAAGTACACCGCGATACCCACAGTTTTAAAAA
CGCGCTGCGCAGCGCATTGCGTGAAGATCCGGATGTGATTCTGGTCGGTGAGCTGCGCGATCAGGAAACCATCAGCTTAG
CGCTGACGGCGGCGGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCAGTTCGGCGGCGAAAACCATTGACCGCATC
ATCGATGTGTTCCCGGGCAGCGACAAGGACATGGTACGTTCAATGTTGTCAGAATCGCTGCGTGCGGTGATTGCGCAAAA
ACTGCTCAAACGTCTGGGTGGCGGGCGAGTGGCGTGTCATGAAATCATGTTGGCGACGCCTGCTATCCGTAACCTGATTC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACTGGCGCCTCCCATGGAATGCAGACTATGGAGCAGCATGCC
AAACAGTTGATCGCTCAGGGGCTGGTGGATCCGGCGGAAGTGCAGAAGAAAATTGAACTGGAAATGTCCGCGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

92.754

100

0.928
  pilT Vibrio cholerae strain A1552

92.754

100

0.928
  pilT Acinetobacter baumannii D1279779

71.601

95.942

0.687
  pilT Acinetobacter baumannii strain A118

71.601

95.942

0.687
  pilT Acinetobacter nosocomialis M2

71.299

95.942

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.059

98.551

0.661
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

62.059

98.551

0.612
  pilT Neisseria gonorrhoeae MS11

61.765

98.551

0.609
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.424

95.652

0.501
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.867

96.232

0.403
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374