Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PWS06_RS04295 Genome accession   NZ_CP118532
Coordinates   880807..881268 (-) Length   153 a.a.
NCBI ID   WP_050940736.1    Uniprot ID   -
Organism   Vibrio harveyi strain 160-19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 875807..886268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWS06_RS04275 coaE 876302..876916 (-) 615 WP_274790538.1 dephospho-CoA kinase -
  PWS06_RS04280 pilD 876917..877786 (-) 870 WP_045488712.1 A24 family peptidase Machinery gene
  PWS06_RS04285 pilC 877859..879082 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWS06_RS04290 pilB 879116..880801 (-) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWS06_RS04295 pilA 880807..881268 (-) 462 WP_050940736.1 pilin Machinery gene
  PWS06_RS04300 nadC 881533..882420 (-) 888 WP_045393857.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWS06_RS04305 ampD 882513..883064 (+) 552 WP_050936250.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWS06_RS04310 pdhR 883469..884236 (+) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15846.05 Da        Isoelectric Point: 8.4742

>NTDB_id=792984 PWS06_RS04295 WP_050940736.1 880807..881268(-) (pilA) [Vibrio harveyi strain 160-19]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQKYVAKSEVASALATLTGVKTNVEAYAVENGAFPDGSTANQTEAD
LGVPSSIPSGGITFTKGASAATGTITFKFVATGVSNLIAGKQFDLKRDGNGTWTCDGSATTPVSDDVLPKNCR

Nucleotide


Download         Length: 462 bp        

>NTDB_id=792984 PWS06_RS04295 WP_050940736.1 880807..881268(-) (pilA) [Vibrio harveyi strain 160-19]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTGATGATAGTGGTAGCAGTTATTGGTGTATT
AGCAGCGATAGCAATTCCTCAATATCAAAAATATGTAGCGAAATCAGAAGTCGCATCTGCATTAGCTACATTAACTGGTG
TAAAAACTAACGTTGAAGCTTACGCTGTAGAAAATGGTGCTTTTCCTGATGGTTCAACAGCTAACCAAACAGAAGCAGAT
CTAGGTGTACCTAGCTCAATTCCTTCCGGTGGAATAACTTTCACAAAAGGGGCATCAGCGGCAACTGGTACTATTACCTT
TAAATTTGTAGCAACTGGTGTAAGCAACCTAATTGCAGGGAAGCAATTCGATTTGAAAAGAGATGGTAACGGAACATGGA
CTTGTGATGGTTCTGCGACGACTCCAGTATCAGATGATGTACTACCAAAGAACTGTCGCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.436

100

0.484
  pilA Vibrio cholerae strain A1552

47.436

100

0.484
  pilA Vibrio cholerae C6706

47.436

100

0.484
  pilA Pseudomonas aeruginosa PAK

42.484

100

0.425
  pilA Vibrio parahaemolyticus RIMD 2210633

45.324

90.85

0.412
  pilA Acinetobacter baumannii strain A118

43.448

94.771

0.412
  pilA Ralstonia pseudosolanacearum GMI1000

32.759

100

0.373
  pilE Neisseria gonorrhoeae strain FA1090

34.783

100

0.366
  pilE Neisseria gonorrhoeae MS11

34.568

100

0.366
  pilA2 Legionella pneumophila str. Paris

36.129

100

0.366
  pilA2 Legionella pneumophila strain ERS1305867

36.129

100

0.366
  pilA/pilA1 Eikenella corrodens VA1

33.939

100

0.366