Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PUT85_RS02700 Genome accession   NZ_CP118530
Coordinates   536353..536814 (-) Length   153 a.a.
NCBI ID   WP_050940736.1    Uniprot ID   -
Organism   Vibrio harveyi strain A2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 531353..541814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUT85_RS02680 coaE 531848..532462 (-) 615 WP_017817675.1 dephospho-CoA kinase -
  PUT85_RS02685 pilD 532463..533332 (-) 870 WP_045488712.1 A24 family peptidase Machinery gene
  PUT85_RS02690 pilC 533405..534628 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PUT85_RS02695 pilB 534662..536347 (-) 1686 WP_017189132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PUT85_RS02700 pilA 536353..536814 (-) 462 WP_050940736.1 pilin Machinery gene
  PUT85_RS02705 nadC 537079..537966 (-) 888 WP_045393857.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PUT85_RS02710 ampD 538059..538610 (+) 552 WP_050936250.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PUT85_RS02715 pdhR 539015..539782 (+) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15846.05 Da        Isoelectric Point: 8.4742

>NTDB_id=792928 PUT85_RS02700 WP_050940736.1 536353..536814(-) (pilA) [Vibrio harveyi strain A2]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQKYVAKSEVASALATLTGVKTNVEAYAVENGAFPDGSTANQTEAD
LGVPSSIPSGGITFTKGASAATGTITFKFVATGVSNLIAGKQFDLKRDGNGTWTCDGSATTPVSDDVLPKNCR

Nucleotide


Download         Length: 462 bp        

>NTDB_id=792928 PUT85_RS02700 WP_050940736.1 536353..536814(-) (pilA) [Vibrio harveyi strain A2]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTGATGATAGTGGTAGCAGTTATTGGTGTATT
AGCAGCGATAGCAATTCCTCAATATCAAAAATATGTAGCGAAATCAGAAGTCGCATCTGCATTAGCTACATTAACTGGTG
TAAAAACTAACGTTGAAGCTTACGCTGTAGAAAATGGTGCTTTTCCTGATGGTTCAACAGCTAACCAAACAGAAGCAGAT
CTAGGTGTACCTAGCTCAATTCCTTCCGGTGGAATAACTTTCACAAAAGGGGCATCAGCGGCAACTGGTACTATTACCTT
TAAATTTGTAGCAACTGGTGTAAGCAACCTAATTGCAGGGAAGCAATTCGATTTGAAAAGAGATGGTAACGGAACATGGA
CTTGTGATGGTTCTGCGACGACTCCAGTATCAGATGATGTACTACCAAAGAACTGTCGCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.436

100

0.484
  pilA Vibrio cholerae strain A1552

47.436

100

0.484
  pilA Vibrio cholerae C6706

47.436

100

0.484
  pilA Pseudomonas aeruginosa PAK

42.484

100

0.425
  pilA Vibrio parahaemolyticus RIMD 2210633

45.324

90.85

0.412
  pilA Acinetobacter baumannii strain A118

43.448

94.771

0.412
  pilA Ralstonia pseudosolanacearum GMI1000

32.759

100

0.373
  pilE Neisseria gonorrhoeae strain FA1090

34.783

100

0.366
  pilE Neisseria gonorrhoeae MS11

34.568

100

0.366
  pilA2 Legionella pneumophila str. Paris

36.129

100

0.366
  pilA2 Legionella pneumophila strain ERS1305867

36.129

100

0.366
  pilA/pilA1 Eikenella corrodens VA1

33.939

100

0.366