Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   PUW49_RS00990 Genome accession   NZ_CP118054
Coordinates   185476..186417 (+) Length   313 a.a.
NCBI ID   WP_024053130.1    Uniprot ID   -
Organism   Streptococcus anginosus strain VSI37     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 180476..191417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW49_RS00965 (PUW49_00965) - 180789..181988 (+) 1200 WP_024053134.1 phosphoglycerate kinase -
  PUW49_RS00970 (PUW49_00970) - 182503..183033 (+) 531 WP_070241503.1 aromatic acid exporter family protein -
  PUW49_RS00975 (PUW49_00975) - 183110..183469 (+) 360 WP_003070793.1 MerR family transcriptional regulator -
  PUW49_RS00980 (PUW49_00980) glnA 183507..184853 (+) 1347 WP_024053132.1 type I glutamate--ammonia ligase -
  PUW49_RS00985 (PUW49_00985) - 185041..185406 (+) 366 WP_024053131.1 DUF1033 family protein -
  PUW49_RS00990 (PUW49_00990) comYA 185476..186417 (+) 942 WP_024053130.1 competence type IV pilus ATPase ComGA Machinery gene
  PUW49_RS00995 (PUW49_00995) comYB 186359..187381 (+) 1023 WP_274994772.1 competence type IV pilus assembly protein ComGB Machinery gene
  PUW49_RS01000 (PUW49_01000) comYC 187378..187695 (+) 318 WP_003028473.1 competence type IV pilus major pilin ComGC Machinery gene
  PUW49_RS01005 (PUW49_01005) comYD 187685..188083 (+) 399 WP_022525973.1 competence type IV pilus minor pilin ComGD Machinery gene
  PUW49_RS01010 (PUW49_01010) comGE/cglE 188055..188348 (+) 294 WP_024053127.1 competence type IV pilus minor pilin ComGE Machinery gene
  PUW49_RS01015 (PUW49_01015) comGF/cglF 188332..188769 (+) 438 WP_024053126.1 competence type IV pilus minor pilin ComGF Machinery gene
  PUW49_RS01020 (PUW49_01020) comGG 188750..189076 (+) 327 WP_257360478.1 competence type IV pilus minor pilin ComGG -
  PUW49_RS01025 (PUW49_01025) comYH 189148..190101 (+) 954 WP_024053125.1 class I SAM-dependent methyltransferase Machinery gene
  PUW49_RS01030 (PUW49_01030) - 190148..191347 (+) 1200 WP_024053124.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35929.17 Da        Isoelectric Point: 6.2451

>NTDB_id=789639 PUW49_RS00990 WP_024053130.1 185476..186417(+) (comYA) [Streptococcus anginosus strain VSI37]
MVQNIAQAIICQAKEERAQDIYFVPKENVYELYMRIGDERRFIRNYEFEEMAAVISHFKFTAGMNVGEKRRSQLGSCDYQ
YGDKKTSLRLSTVGDYRGYESLVIRLLHDEEAELKFWFAQIPELKERFKQRGLYLFSGPVGSGKTTLMHQLAQERFADQQ
IMSIEDPVEIKQAGMLQLQLNEAIGLTYESLIKLSLRHRPDLLIIGEIRDAETARAVVRASLTGMTVFSTIHAKSVRGVY
ERLLELGVTEAELKVVLQGICYQRLIAKGGIVDFVSEKYQEHEPIDWNRQIDQLHAAGHINLEQAEAEKIIHS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=789639 PUW49_RS00990 WP_024053130.1 185476..186417(+) (comYA) [Streptococcus anginosus strain VSI37]
ATGGTTCAAAACATTGCACAGGCTATTATTTGTCAGGCTAAGGAAGAACGAGCACAGGACATTTATTTTGTTCCGAAGGA
AAATGTTTACGAGTTGTATATGCGAATTGGAGATGAACGCCGTTTTATACGCAATTATGAATTTGAAGAGATGGCAGCAG
TAATTAGTCATTTTAAGTTTACTGCTGGGATGAATGTCGGCGAAAAACGTAGAAGCCAGTTAGGTTCTTGTGATTATCAA
TATGGTGATAAGAAAACGTCATTACGACTATCAACAGTAGGGGATTATCGTGGATACGAAAGTCTGGTGATTCGTTTATT
GCATGATGAAGAGGCAGAGTTGAAGTTTTGGTTTGCTCAAATCCCAGAGTTAAAAGAACGCTTCAAACAACGAGGGCTTT
ATCTTTTTTCGGGACCGGTAGGAAGTGGTAAAACTACTCTCATGCACCAGTTGGCTCAAGAGCGTTTTGCCGATCAGCAA
ATCATGTCAATTGAAGATCCGGTTGAAATCAAACAAGCAGGGATGTTACAGCTGCAGCTGAACGAAGCGATTGGTTTAAC
TTATGAAAGTTTGATTAAACTTTCTCTTCGGCATCGACCAGATTTATTGATTATCGGAGAGATTCGTGATGCAGAGACAG
CAAGGGCAGTTGTTCGAGCTAGCTTAACGGGAATGACTGTTTTTTCAACTATTCATGCTAAAAGCGTACGAGGTGTCTAT
GAACGGTTGCTGGAGTTGGGAGTGACAGAAGCCGAGCTGAAAGTTGTCTTACAAGGGATTTGTTACCAACGCTTAATTGC
GAAAGGAGGAATAGTGGATTTTGTCAGTGAGAAGTATCAAGAACACGAGCCAATCGATTGGAATAGGCAGATTGATCAGC
TTCATGCAGCAGGACATATCAATCTTGAGCAGGCAGAAGCGGAAAAAATTATCCACAGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus gordonii str. Challis substr. CH1

82.903

99.042

0.821
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

75.719

100

0.757
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

75.399

100

0.754
  comGA/cglA/cilD Streptococcus pneumoniae D39

75.399

100

0.754
  comGA/cglA/cilD Streptococcus pneumoniae R6

75.399

100

0.754
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

75.399

100

0.754
  comYA Streptococcus mutans UA159

64.63

99.361

0.642
  comYA Streptococcus mutans UA140

64.63

99.361

0.642
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.341

99.361

0.54
  comGA Latilactobacillus sakei subsp. sakei 23K

44.697

84.345

0.377