Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   PUW49_RS00995 Genome accession   NZ_CP118054
Coordinates   186359..187381 (+) Length   340 a.a.
NCBI ID   WP_274994772.1    Uniprot ID   -
Organism   Streptococcus anginosus strain VSI37     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 181359..192381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW49_RS00970 (PUW49_00970) - 182503..183033 (+) 531 WP_070241503.1 aromatic acid exporter family protein -
  PUW49_RS00975 (PUW49_00975) - 183110..183469 (+) 360 WP_003070793.1 MerR family transcriptional regulator -
  PUW49_RS00980 (PUW49_00980) glnA 183507..184853 (+) 1347 WP_024053132.1 type I glutamate--ammonia ligase -
  PUW49_RS00985 (PUW49_00985) - 185041..185406 (+) 366 WP_024053131.1 DUF1033 family protein -
  PUW49_RS00990 (PUW49_00990) comYA 185476..186417 (+) 942 WP_024053130.1 competence type IV pilus ATPase ComGA Machinery gene
  PUW49_RS00995 (PUW49_00995) comYB 186359..187381 (+) 1023 WP_274994772.1 competence type IV pilus assembly protein ComGB Machinery gene
  PUW49_RS01000 (PUW49_01000) comYC 187378..187695 (+) 318 WP_003028473.1 competence type IV pilus major pilin ComGC Machinery gene
  PUW49_RS01005 (PUW49_01005) comYD 187685..188083 (+) 399 WP_022525973.1 competence type IV pilus minor pilin ComGD Machinery gene
  PUW49_RS01010 (PUW49_01010) comGE/cglE 188055..188348 (+) 294 WP_024053127.1 competence type IV pilus minor pilin ComGE Machinery gene
  PUW49_RS01015 (PUW49_01015) comGF/cglF 188332..188769 (+) 438 WP_024053126.1 competence type IV pilus minor pilin ComGF Machinery gene
  PUW49_RS01020 (PUW49_01020) comGG 188750..189076 (+) 327 WP_257360478.1 competence type IV pilus minor pilin ComGG -
  PUW49_RS01025 (PUW49_01025) comYH 189148..190101 (+) 954 WP_024053125.1 class I SAM-dependent methyltransferase Machinery gene
  PUW49_RS01030 (PUW49_01030) - 190148..191347 (+) 1200 WP_024053124.1 acetate kinase -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 39196.34 Da        Isoelectric Point: 9.5495

>NTDB_id=789640 PUW49_RS00995 WP_274994772.1 186359..187381(+) (comYB) [Streptococcus anginosus strain VSI37]
MQQDISILSRQKRKKLSTVRQKKVVELLNNLFSSGFHLAEIIDFLKRSALLEEVYVDRMKEELSTGKPFSDIMASLGFSD
NVVTQLSLAELHGNLSLSLSKIEEYLENISKIKKKLVEVPTYPFILFVFLIFIMLGLRNYLLPQLDRQNIATQIISNLPQ
LFLGSLGVVFVLLAIGFYWFQKSSKIAVFRLLARLPFLGAFIQTYLTAYYAREWGNMIGQGLELSQIFQMMQGQRSAMFQ
EIGKDLEVALQNGQEFSQRVKTYPFFKKELGLMIEYGEVKSKLGCELEVYAQKTWETFFKRVHQAMNVIQPLVFVFVALM
IVLLYAAMLLPIYQNMEVQL

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=789640 PUW49_RS00995 WP_274994772.1 186359..187381(+) (comYB) [Streptococcus anginosus strain VSI37]
ATGCAGCAGGACATATCAATCTTGAGCAGGCAGAAGCGGAAAAAATTATCCACAGTTAGGCAGAAAAAGGTCGTAGAGTT
GCTGAATAATCTTTTTTCTAGTGGCTTTCATTTGGCAGAAATTATCGACTTTTTGAAGCGAAGTGCCTTGTTAGAAGAAG
TCTATGTGGACAGGATGAAAGAGGAGTTGTCAACAGGAAAGCCTTTTTCGGACATTATGGCAAGCTTAGGTTTTTCAGAT
AACGTGGTGACACAGCTTTCGCTGGCAGAATTACACGGAAATCTCTCACTCAGCCTGAGTAAGATTGAGGAATACTTGGA
AAATATCTCAAAAATTAAGAAGAAGTTGGTTGAAGTGCCGACTTATCCCTTTATTTTGTTTGTTTTTTTGATATTCATTA
TGCTGGGATTACGTAATTATTTACTCCCGCAACTCGATCGTCAAAATATAGCAACTCAGATTATCAGTAATTTGCCGCAA
CTTTTTCTGGGTTCATTAGGGGTAGTCTTTGTTTTGTTAGCGATAGGTTTTTATTGGTTTCAAAAAAGCTCAAAAATAGC
AGTATTTCGTTTGTTAGCTCGCCTTCCTTTTTTGGGCGCTTTTATTCAAACCTATTTAACAGCTTATTATGCGAGAGAAT
GGGGGAATATGATTGGTCAAGGTTTAGAACTCAGTCAGATTTTTCAGATGATGCAAGGACAACGCTCTGCTATGTTTCAA
GAAATTGGAAAAGATTTAGAAGTTGCGCTGCAAAATGGTCAGGAATTTTCGCAAAGAGTTAAAACATATCCATTTTTTAA
AAAGGAATTGGGCTTGATGATCGAATACGGTGAAGTAAAATCCAAATTGGGGTGTGAATTAGAAGTTTATGCCCAAAAAA
CATGGGAGACATTTTTTAAACGTGTTCATCAAGCGATGAATGTCATTCAACCACTAGTGTTTGTCTTTGTGGCTTTAATG
ATTGTATTGTTGTATGCAGCCATGTTGCTGCCAATTTATCAAAATATGGAGGTTCAATTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(26-143)

Type II secretion system (T2SS), protein F (T2SSF)

(211-331)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus gordonii str. Challis substr. CH1

70

100

0.7
  comGB/cglB Streptococcus mitis SK321

68.358

98.529

0.674
  comGB/cglB Streptococcus mitis NCTC 12261

68.358

98.529

0.674
  comGB/cglB Streptococcus pneumoniae Rx1

67.463

98.529

0.665
  comGB/cglB Streptococcus pneumoniae D39

67.463

98.529

0.665
  comGB/cglB Streptococcus pneumoniae R6

67.463

98.529

0.665
  comGB/cglB Streptococcus pneumoniae TIGR4

67.463

98.529

0.665
  comYB Streptococcus mutans UA159

59.942

100

0.603
  comYB Streptococcus mutans UA140

59.649

100

0.6
  comGB Lactococcus lactis subsp. cremoris KW2

50.742

99.118

0.503