Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QY054_RS12180 Genome accession   NZ_CP130602
Coordinates   2416389..2417582 (+) Length   397 a.a.
NCBI ID   WP_011244064.1    Uniprot ID   Q31KP0
Organism   Synechococcus elongatus PCC 7942 = FACHB-805 strain UTEX 2434     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2411389..2422582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QY054_RS12155 (QY054_12155) - 2411501..2412451 (+) 951 WP_011378416.1 HEAT repeat domain-containing protein -
  QY054_RS12160 (QY054_12160) - 2412590..2412928 (-) 339 WP_011244068.1 30S ribosomal protein PSRP-3 -
  QY054_RS12165 (QY054_12165) - 2413343..2414143 (+) 801 WP_011244067.1 TIGR01548 family HAD-type hydrolase -
  QY054_RS12170 (QY054_12170) - 2414140..2415165 (-) 1026 WP_321167205.1 cupin -
  QY054_RS12175 (QY054_12175) queA 2415235..2416314 (+) 1080 WP_011378419.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  QY054_RS12180 (QY054_12180) pilT 2416389..2417582 (+) 1194 WP_011244064.1 type IV pilus twitching motility protein PilT Machinery gene
  QY054_RS12185 (QY054_12185) - 2417595..2418296 (-) 702 WP_011244063.1 L-threonylcarbamoyladenylate synthase -
  QY054_RS12190 (QY054_12190) larC 2418312..2419520 (+) 1209 WP_011378420.1 nickel pincer cofactor biosynthesis protein LarC -
  QY054_RS12195 (QY054_12195) raiA 2419577..2420200 (-) 624 WP_011378421.1 ribosome-associated translation inhibitor RaiA -
  QY054_RS12200 (QY054_12200) lipB 2420406..2421107 (+) 702 WP_011244060.1 lipoyl(octanoyl) transferase LipB -
  QY054_RS12205 (QY054_12205) - 2421204..2421728 (-) 525 WP_011244059.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  QY054_RS12210 (QY054_12210) - 2421781..2422104 (-) 324 WP_011244058.1 phasin family protein -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 43976.36 Da        Isoelectric Point: 5.6795

>NTDB_id=785993 QY054_RS12180 WP_011244064.1 2416389..2417582(+) (pilT) [Synechococcus elongatus PCC 7942 = FACHB-805 strain UTEX 2434]
MTDTSRLPLPPPPPPFGAGGLSAMPVTQVSASPATPAAVPQRSANQPTLAEIVRQAYEHDYSDVHLGVGEVPRFRDRGEI
VQTTYPVTDTATFNAWLREVLTEAQVREFEQGLDFDGATQYDFARVRINIFGSLRGPSMVMRLIPLRILSLDELSLPPVF
RDICYYPKGLVLVTGPTGSGKSTTLAAMIDFINQEMAKNIITIEDPIEFVHQSQRSLIKQREVGVHTRQFEAALRASLRE
DPDIILVGELRDRETINTALKAAQTGHLVMATLHTNSAVKTIERVLNMFEPAEQPPVRVALAESLVSVIAQGLCRTIHGK
RTAYHEILINTDAIKDYIIRGELEEVEQLIPKCTFDGMCTSNQSLFRLFNEGKISEETALAQSGKPNEMSQMLRGRV

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=785993 QY054_RS12180 WP_011244064.1 2416389..2417582(+) (pilT) [Synechococcus elongatus PCC 7942 = FACHB-805 strain UTEX 2434]
ATGACTGATACCTCGCGCCTGCCACTCCCACCGCCTCCCCCTCCCTTTGGTGCAGGTGGACTGTCTGCGATGCCCGTCAC
CCAGGTCAGCGCTTCCCCGGCAACCCCAGCAGCCGTGCCCCAACGATCGGCCAATCAGCCAACCCTTGCCGAAATTGTGC
GGCAGGCCTACGAGCACGATTACTCGGATGTCCACCTTGGCGTGGGGGAAGTGCCGCGTTTTCGCGATCGCGGTGAAATT
GTGCAAACAACCTATCCAGTCACCGACACAGCTACCTTTAACGCCTGGCTGCGCGAGGTTTTGACCGAAGCGCAAGTGCG
TGAATTTGAGCAGGGATTGGACTTCGACGGCGCAACTCAGTACGACTTTGCGCGGGTACGGATCAACATCTTTGGGTCGC
TGCGAGGTCCCTCGATGGTGATGCGGTTAATTCCGCTGCGCATTCTCAGCCTCGATGAGCTGAGCCTACCGCCGGTTTTC
CGCGACATCTGCTACTACCCCAAAGGACTGGTACTGGTCACCGGCCCCACAGGGTCAGGCAAGTCGACCACGCTGGCCGC
CATGATTGATTTCATCAATCAGGAAATGGCGAAGAATATCATCACCATCGAAGACCCGATTGAATTTGTTCACCAAAGTC
AGCGATCGCTCATTAAGCAGCGGGAAGTCGGCGTTCATACCCGTCAATTTGAAGCAGCACTACGGGCCTCTTTGCGTGAG
GATCCTGACATTATCTTGGTGGGTGAGCTGCGCGATCGCGAAACGATCAATACCGCTCTCAAAGCTGCACAAACCGGCCA
CCTCGTGATGGCTACTCTGCACACTAACAGCGCTGTCAAAACAATTGAGCGGGTACTCAATATGTTTGAACCCGCTGAAC
AACCGCCTGTTCGAGTTGCCTTGGCCGAGTCGCTCGTGTCTGTGATTGCGCAAGGTCTCTGCCGCACGATTCACGGCAAG
CGGACTGCCTATCACGAAATTTTGATTAACACTGACGCGATCAAGGACTACATTATTCGCGGCGAACTTGAGGAAGTTGA
GCAACTAATTCCTAAATGTACCTTTGACGGCATGTGCACCAGCAATCAGTCCCTATTCCGTCTCTTTAACGAAGGCAAAA
TTAGTGAGGAGACTGCACTGGCGCAATCTGGCAAGCCCAATGAAATGTCTCAGATGCTGCGAGGTCGGGTTTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(52-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q31KP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

46.313

85.39

0.395
  pilT Acinetobacter nosocomialis M2

45.428

85.39

0.388
  pilT Acinetobacter baumannii D1279779

45.428

85.39

0.388
  pilT Acinetobacter baumannii strain A118

45.428

85.39

0.388
  pilT Neisseria meningitidis 8013

45.075

84.383

0.38
  pilT Pseudomonas aeruginosa PAK

44.543

85.39

0.38
  pilT Pseudomonas stutzeri DSM 10701

44.248

85.39

0.378
  pilT Neisseria gonorrhoeae MS11

44.776

84.383

0.378
  pilT Legionella pneumophila strain Lp02

44.88

83.627

0.375
  pilT Legionella pneumophila strain ERS1305867

44.88

83.627

0.375
  pilT Vibrio cholerae strain A1552

44.848

83.123

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.848

83.123

0.373
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.499

87.406

0.363