Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PSH72_RS24400 Genome accession   NZ_CP117453
Coordinates   5400892..5401314 (-) Length   140 a.a.
NCBI ID   WP_305446926.1    Uniprot ID   -
Organism   Pseudomonas cucumis strain FP1962     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5395892..5406314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH72_RS24385 (PSH72_24380) - 5396753..5399743 (+) 2991 WP_305503313.1 DUF748 domain-containing protein -
  PSH72_RS24390 (PSH72_24385) - 5399743..5400039 (+) 297 WP_305449860.1 DUF2845 domain-containing protein -
  PSH72_RS24395 (PSH72_24390) - 5400219..5400575 (-) 357 WP_305503315.1 BON domain-containing protein -
  PSH72_RS24400 (PSH72_24395) pilA/pilAI 5400892..5401314 (-) 423 WP_305446926.1 pilin Machinery gene
  PSH72_RS24405 (PSH72_24400) pilB 5401541..5403241 (+) 1701 WP_305446927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH72_RS24410 (PSH72_24405) pilC 5403244..5404461 (+) 1218 WP_305503317.1 type II secretion system F family protein Machinery gene
  PSH72_RS24415 (PSH72_24410) pilD 5404463..5405335 (+) 873 WP_305503318.1 A24 family peptidase Machinery gene
  PSH72_RS24420 (PSH72_24415) coaE 5405332..5405955 (+) 624 WP_305425147.1 dephospho-CoA kinase -
  PSH72_RS24425 (PSH72_24420) yacG 5405952..5406152 (+) 201 WP_030130376.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14114.36 Da        Isoelectric Point: 8.1079

>NTDB_id=785509 PSH72_RS24400 WP_305446926.1 5400892..5401314(-) (pilA/pilAI) [Pseudomonas cucumis strain FP1962]
MKNQKGFTLIELLIVVAIIGILATFALPQYSKYQARAKVTAALAEISALKVPFEDIINQGTNPTLALIGGTATTGNCTIT
ASGTAADGAGTIGCVILNAPGPVLSKTITLTRTLTGGWTCATTAEQEYAPKGCTGAAAAG

Nucleotide


Download         Length: 423 bp        

>NTDB_id=785509 PSH72_RS24400 WP_305446926.1 5400892..5401314(-) (pilA/pilAI) [Pseudomonas cucumis strain FP1962]
ATGAAAAATCAAAAAGGTTTTACTCTGATCGAGCTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACGTTTGCGTT
GCCGCAGTATTCGAAATATCAAGCGCGAGCGAAGGTTACCGCTGCTCTTGCAGAAATTTCGGCATTGAAGGTGCCTTTCG
AAGACATTATCAACCAGGGCACTAACCCCACCCTCGCACTGATCGGCGGTACGGCGACCACCGGTAATTGCACGATTACG
GCATCGGGTACCGCCGCTGACGGTGCGGGTACTATTGGTTGCGTTATTCTCAATGCTCCAGGGCCGGTGCTCAGCAAAAC
CATTACGCTTACACGCACCCTTACCGGTGGCTGGACATGCGCCACCACAGCTGAACAAGAATACGCGCCTAAAGGCTGCA
CTGGCGCTGCCGCTGCTGGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50

98.571

0.493
  pilA Acinetobacter baumannii strain A118

47.101

98.571

0.464
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.443
  comP Acinetobacter baylyi ADP1

40.132

100

0.436
  pilA Vibrio cholerae C6706

42.069

100

0.436
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.069

100

0.436
  pilA Vibrio cholerae strain A1552

42.069

100

0.436
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA2 Legionella pneumophila strain ERS1305867

40.972

100

0.421
  pilA2 Legionella pneumophila str. Paris

40.278

100

0.414
  pilA Vibrio parahaemolyticus RIMD 2210633

36.957

98.571

0.364