Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH72_RS24410 Genome accession   NZ_CP117453
Coordinates   5403244..5404461 (+) Length   405 a.a.
NCBI ID   WP_305503317.1    Uniprot ID   -
Organism   Pseudomonas cucumis strain FP1962     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5398244..5409461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH72_RS24390 (PSH72_24385) - 5399743..5400039 (+) 297 WP_305449860.1 DUF2845 domain-containing protein -
  PSH72_RS24395 (PSH72_24390) - 5400219..5400575 (-) 357 WP_305503315.1 BON domain-containing protein -
  PSH72_RS24400 (PSH72_24395) pilA/pilAI 5400892..5401314 (-) 423 WP_305446926.1 pilin Machinery gene
  PSH72_RS24405 (PSH72_24400) pilB 5401541..5403241 (+) 1701 WP_305446927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH72_RS24410 (PSH72_24405) pilC 5403244..5404461 (+) 1218 WP_305503317.1 type II secretion system F family protein Machinery gene
  PSH72_RS24415 (PSH72_24410) pilD 5404463..5405335 (+) 873 WP_305503318.1 A24 family peptidase Machinery gene
  PSH72_RS24420 (PSH72_24415) coaE 5405332..5405955 (+) 624 WP_305425147.1 dephospho-CoA kinase -
  PSH72_RS24425 (PSH72_24420) yacG 5405952..5406152 (+) 201 WP_030130376.1 DNA gyrase inhibitor YacG -
  PSH72_RS24430 (PSH72_24425) - 5406162..5406377 (-) 216 WP_007938959.1 hypothetical protein -
  PSH72_RS24435 (PSH72_24430) - 5406441..5407130 (-) 690 WP_305503320.1 energy-coupling factor ABC transporter permease -
  PSH72_RS24440 (PSH72_24435) - 5407377..5408831 (+) 1455 WP_305503322.1 TrkH family potassium uptake protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44151.13 Da        Isoelectric Point: 9.9046

>NTDB_id=785511 PSH72_RS24410 WP_305503317.1 5403244..5404461(+) (pilC) [Pseudomonas cucumis strain FP1962]
MAVKAAKISIYAWEGTDKKGTKMTGELTGQNPALIKAQLRKQGINPGKVRKKTASIFSAGKRIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFSGFGAELPAFTVMVIGLSEFLQAWWWMVLGMLGALVFGVR
HAFKKSPGFRDRMDTWLLKLPLVGALMYKSAVARYARTLSTTFAAGVPLVEALESVAGATGNIVFKRAVLRIKQDVSTGM
QLNFAMRTSGIFPNMAIQMTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=785511 PSH72_RS24410 WP_305503317.1 5403244..5404461(+) (pilC) [Pseudomonas cucumis strain FP1962]
ATGGCGGTCAAAGCAGCGAAAATCAGCATCTATGCCTGGGAAGGCACAGACAAGAAAGGCACAAAAATGACCGGCGAGTT
GACCGGCCAGAATCCCGCGCTGATCAAGGCCCAGTTACGCAAGCAAGGGATCAACCCGGGCAAGGTGCGGAAAAAAACCG
CGTCCATTTTCAGTGCCGGAAAACGCATCAAGCCCCTGGACATTGCCCTGTTTACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTACCGCTGCTGCAATCGTTCGACATTATCGGCGAGGGCTTCGATAACCCGAACATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCGGCCGGTAACAGCTTCGCCGCGGCCCTGCGCAAAAAGCCCCAGTATTTCGATGAGCTGTACT
GCAACCTGGTGGATGCCGGCGAGCAAGCTGGCGCCCTGGATACCCTGCTGGAACGGGTGGCGACCTATAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACTTACCCGCTGGCGGTGGTGTTCGTCGCGATCATTGTGACCGGGAT
CCTGCTGGTCAAGGTTGTGCCGCAATTCGAGTCAGTGTTCTCGGGGTTTGGCGCCGAGCTGCCGGCGTTCACCGTGATGG
TCATCGGTTTGTCGGAGTTCCTGCAGGCCTGGTGGTGGATGGTGCTCGGCATGCTGGGTGCGCTGGTCTTCGGCGTCCGC
CATGCCTTTAAAAAGTCCCCGGGCTTTCGGGACCGGATGGACACCTGGCTGCTGAAACTGCCGTTGGTTGGCGCGCTCAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACCTTGTCGACGACGTTTGCCGCCGGGGTGCCACTGGTCGAAGCCCTGG
AGTCAGTGGCGGGCGCGACTGGCAACATCGTGTTCAAGCGAGCGGTGTTACGCATCAAGCAGGACGTTTCAACTGGCATG
CAGCTGAATTTTGCCATGCGCACCTCCGGCATCTTTCCGAACATGGCGATCCAGATGACGGCCATCGGCGAAGAGTCCGG
CGCACTGGACGACATGCTCGACAAGGTCGCAAGTTTCTATGAGGACGAAGTGGATAACATGGTCGATAACCTGACCAGTC
TGATGGAGCCTTTCATCATGGTGGTGCTGGGGGTTATCGTCGGCGGCCTGGTGGTTGCGATGTACCTGCCCATCTTCCAA
CTTGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.384

100

0.746
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Acinetobacter baumannii D1279779

58.824

100

0.593
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.523

100

0.417
  pilC Vibrio cholerae strain A1552

42.424

97.778

0.415
  pilC Thermus thermophilus HB27

38.404

99.012

0.38