Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7R81_RS14655 Genome accession   NZ_AP022290
Coordinates   3186123..3187262 (+) Length   379 a.a.
NCBI ID   WP_042057762.1    Uniprot ID   A0A6S5C636
Organism   Aeromonas veronii strain WP9-W18-ESBL-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3181123..3192262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R81_RS14625 (WP9W18E04_28130) - 3181156..3181536 (-) 381 WP_111806690.1 diacylglycerol kinase -
  H7R81_RS14630 (WP9W18E04_28140) - 3181681..3182748 (+) 1068 WP_182969315.1 3-deoxy-7-phosphoheptulonate synthase -
  H7R81_RS14635 (WP9W18E04_28150) - 3182893..3183690 (-) 798 WP_182971072.1 ATP-binding cassette domain-containing protein -
  H7R81_RS14640 (WP9W18E04_28170) btuC 3183756..3184763 (-) 1008 WP_058054056.1 vitamin B12 ABC transporter permease BtuC -
  H7R81_RS14645 (WP9W18E04_28180) - 3184863..3185594 (-) 732 WP_182969316.1 substrate-binding periplasmic protein -
  H7R81_RS14650 (WP9W18E04_28190) - 3185708..3186064 (+) 357 WP_075112061.1 DMT family protein -
  H7R81_RS14655 (WP9W18E04_28200) pilU 3186123..3187262 (+) 1140 WP_042057762.1 type IVa pilus ATPase TapW Machinery gene
  H7R81_RS14660 (WP9W18E04_28210) - 3187359..3187808 (-) 450 WP_182969317.1 methyltransferase family protein -
  H7R81_RS14665 (WP9W18E04_28220) alr 3187982..3189208 (+) 1227 WP_058054059.1 alanine racemase -
  H7R81_RS14670 (WP9W18E04_28230) - 3189258..3190586 (-) 1329 WP_182969318.1 IS4 family transposase -
  H7R81_RS14675 (WP9W18E04_28240) - 3190712..3191656 (-) 945 WP_005353134.1 TerC family protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42280.42 Da        Isoelectric Point: 6.4293

>NTDB_id=77562 H7R81_RS14655 WP_042057762.1 3186123..3187262(+) (pilU) [Aeromonas veronii strain WP9-W18-ESBL-04]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKLQFDKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDMF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=77562 H7R81_RS14655 WP_042057762.1 3186123..3187262(+) (pilU) [Aeromonas veronii strain WP9-W18-ESBL-04]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGGCCTGCGCCCCCTGAATGAAACGCCGCTGGAGCCGGGAGAGGTGGCACGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAACTGCAGTTTGACAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTAGCGCGCAACATCAAGACTGAAATTCCCCGCTTTGA
AGACCTGAAACTTCCGCCCGTGCTGCTCGACACCATCATGGAGAAACGGGGTCTGGTGCTTTTTGTCGGCGGGACCGGCT
CGGGCAAATCCACCTCGCTGGCGGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTCCACCGCCACCGCAAGAGCATCATCAACCAACGGGAGGTGGGGGTCGATACCCGCAGCTTCCACGC
TGCGCTGAAAAACACCCTGCGTCAGGCACCGGATGTGATCCTGATCGGCGAAATCCGCGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAAACAGGGCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCGCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGCCGCCGGGCCGCGGTGGAGATCATGCTGGGTACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGCGGCCTGAAAGAGGTGATGGAAAAATCCAAGGCGCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGAGGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAAATTGCCAGCAGCAGCGATGCCACCGGTTGGAGCCTCGAACCCACCAAGGACG
AGAAGGACGATATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S5C636

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.211

100

0.544
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

89.446

0.372
  pilT Pseudomonas aeruginosa PAK

40.988

90.765

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii D1279779

41.317

88.127

0.364
  pilT Acinetobacter baumannii strain A118

41.317

88.127

0.364
  pilT Acinetobacter nosocomialis M2

41.317

88.127

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364

Multiple sequence alignment