Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PG915_RS02650 Genome accession   NZ_CP115920
Coordinates   579236..580342 (-) Length   368 a.a.
NCBI ID   WP_418642298.1    Uniprot ID   -
Organism   Vibrio chaetopteri strain CB1-14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 574236..585342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG915_RS02620 (PG915_02615) - 576835..578457 (+) 1623 WP_353497756.1 methyl-accepting chemotaxis protein -
  PG915_RS02650 (PG915_02645) pilU 579236..580342 (-) 1107 WP_418642298.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG915_RS02655 (PG915_02650) pilT 580355..581395 (-) 1041 WP_353497758.1 type IV pilus twitching motility protein PilT Machinery gene
  PG915_RS02660 (PG915_02655) - 581421..582131 (+) 711 WP_353497759.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG915_RS02665 (PG915_02660) proC 582170..582988 (+) 819 WP_353497760.1 pyrroline-5-carboxylate reductase -
  PG915_RS02670 (PG915_02665) - 583020..583577 (+) 558 WP_353497761.1 YggT family protein -
  PG915_RS02675 (PG915_02670) yggU 583577..583864 (+) 288 WP_353497762.1 DUF167 family protein YggU -
  PG915_RS02680 (PG915_02675) - 583889..584320 (+) 432 WP_353497763.1 DUF4426 domain-containing protein -
  PG915_RS02685 (PG915_02680) - 584337..584939 (+) 603 WP_353497764.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41162.41 Da        Isoelectric Point: 7.3347

>NTDB_id=774441 PG915_RS02650 WP_418642298.1 579236..580342(-) (pilU) [Vibrio chaetopteri strain CB1-14]
MIIDDVLNHMLELKASDLYLTVGAPVLYRVDGELKSRGETLTERAIHELLNAVMDTNLRQDYLASKEANFAIVKAIGRFR
VSAFFQRELPGAVIRRIETKIPSFEELALPDVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNYNRSGHILTVEDPI
EFVHEHHKCIVTQREVGLDTESYDVALKNSLRQAPDMILIGEIRTRSTMEYAMAFAETGHLCMATLHANNANQALERILH
LVPKERKEQFLFDLSTNLRGIVAQQLIRDKNGRGRHGVFEVLLNSPRVAELIRKGDLHEIKTAMSKSKEVGMQTFDQALY
DLVMANKINQSDALHSADSPNDLRLMLKLQASDSKQQGRLTGIKIDMS

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=774441 PG915_RS02650 WP_418642298.1 579236..580342(-) (pilU) [Vibrio chaetopteri strain CB1-14]
ATAATCATTGATGACGTTCTAAATCACATGCTAGAGCTCAAAGCTTCCGATCTCTATCTAACGGTTGGGGCACCTGTTCT
TTATCGTGTCGACGGGGAGCTAAAATCCCGGGGAGAAACGCTTACCGAACGAGCGATTCACGAACTATTAAACGCCGTCA
TGGATACTAATCTTCGACAAGATTATCTCGCGTCCAAAGAGGCAAACTTCGCGATCGTAAAAGCGATTGGCCGCTTTCGC
GTCAGTGCCTTTTTTCAACGAGAGCTCCCTGGTGCGGTAATACGCCGCATCGAAACCAAAATACCTAGCTTTGAAGAGCT
AGCACTACCGGATGTTCTCCAAGACTTAGCGATCGCGAAACGTGGGCTGGTACTTGTTGTTGGTGCAACCGGTTCTGGAA
AATCAACCACGATGGCTGCGATGACAGGTTATCGCAACTACAATCGAAGTGGACATATCCTAACGGTTGAAGATCCCATT
GAGTTTGTTCATGAGCACCACAAATGCATTGTTACCCAGAGAGAAGTCGGTTTAGACACTGAAAGCTACGACGTCGCCTT
GAAGAATTCGCTGAGGCAAGCACCTGATATGATCCTAATTGGTGAAATTCGCACGCGCTCAACCATGGAATATGCGATGG
CATTCGCAGAAACAGGCCACCTTTGTATGGCGACTCTTCATGCTAATAACGCCAACCAAGCATTGGAAAGAATACTTCAT
CTAGTACCCAAAGAGCGCAAAGAGCAATTTCTGTTTGATCTCTCAACCAACTTAAGAGGCATTGTCGCCCAGCAACTCAT
ACGGGACAAAAATGGTCGCGGTAGACATGGGGTATTCGAAGTTTTACTGAACAGTCCGAGGGTGGCAGAACTCATACGCA
AGGGGGACCTCCACGAAATCAAAACAGCGATGAGCAAAAGCAAGGAAGTCGGAATGCAAACCTTCGACCAAGCCCTATAT
GATCTTGTTATGGCAAATAAAATCAATCAAAGCGATGCTCTTCACAGTGCAGACTCACCAAACGACCTACGACTGATGCT
AAAGCTTCAAGCGAGCGACTCTAAACAACAGGGTCGATTAACTGGTATTAAAATAGATATGAGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

74.932

99.728

0.747
  pilU Pseudomonas stutzeri DSM 10701

55.914

100

0.565
  pilU Acinetobacter baylyi ADP1

52.514

97.283

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.75

0.38
  pilT Legionella pneumophila strain Lp02

41.003

92.12

0.378
  pilT Legionella pneumophila strain ERS1305867

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

40.643

92.935

0.378
  pilT Acinetobacter baumannii D1279779

40.643

92.935

0.378
  pilT Acinetobacter baumannii strain A118

40.643

92.935

0.378
  pilT Acinetobacter nosocomialis M2

40.643

92.935

0.378
  pilT Pseudomonas aeruginosa PAK

39.71

93.75

0.372
  pilT Neisseria gonorrhoeae MS11

40.673

88.859

0.361