Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7R77_RS12945 Genome accession   NZ_AP022254
Coordinates   2822376..2823512 (+) Length   378 a.a.
NCBI ID   WP_029314740.1    Uniprot ID   -
Organism   Aeromonas caviae strain WP8-S18-ESBL-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2817376..2828512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R77_RS12915 (WP8S18E04_25110) - 2817478..2817858 (-) 381 WP_109112397.1 diacylglycerol kinase -
  H7R77_RS12920 (WP8S18E04_25120) - 2818000..2819064 (+) 1065 WP_182975851.1 3-deoxy-7-phosphoheptulonate synthase -
  H7R77_RS12925 (WP8S18E04_25130) - 2819276..2820049 (-) 774 WP_134997365.1 ATP-binding cassette domain-containing protein -
  H7R77_RS12930 (WP8S18E04_25140) btuC 2820036..2821043 (-) 1008 WP_134997356.1 vitamin B12 ABC transporter permease BtuC -
  H7R77_RS12935 (WP8S18E04_25150) - 2821119..2821850 (-) 732 WP_182975852.1 ABC transporter substrate-binding protein -
  H7R77_RS12940 (WP8S18E04_25160) - 2821963..2822319 (+) 357 WP_010675200.1 DMT family protein -
  H7R77_RS12945 (WP8S18E04_25170) pilU 2822376..2823512 (+) 1137 WP_029314740.1 type IVa pilus ATPase TapW Machinery gene
  H7R77_RS12950 (WP8S18E04_25180) - 2823574..2823816 (-) 243 WP_010675202.1 FeoC-like transcriptional regulator -
  H7R77_RS12955 (WP8S18E04_25190) feoB 2823863..2826133 (-) 2271 WP_134997355.1 Fe(2+) transporter permease subunit FeoB -
  H7R77_RS12960 (WP8S18E04_25200) - 2826130..2826357 (-) 228 WP_010675204.1 FeoA family protein -
  H7R77_RS12965 (WP8S18E04_25210) - 2826477..2826806 (+) 330 WP_182975853.1 translation initiation factor -
  H7R77_RS12970 (WP8S18E04_25220) - 2827060..2827617 (+) 558 WP_010675206.1 TMEM165/GDT1 family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42203.27 Da        Isoelectric Point: 6.6000

>NTDB_id=77416 H7R77_RS12945 WP_029314740.1 2822376..2823512(+) (pilU) [Aeromonas caviae strain WP8-S18-ESBL-04]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLGPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=77416 H7R77_RS12945 WP_029314740.1 2822376..2823512(+) (pilU) [Aeromonas caviae strain WP8-S18-ESBL-04]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTCACCCAGGAGCCCCTGGGGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTTTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCTCCGGTGCTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAATCCACATCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACACGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGACAGGCACCGGACGTCATCCTCATCGGTGAAATCCGCGATCGGGAGACCATGGAGCATG
CCCTCGCCTTCTCGGAAACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAACTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGGCGCCGGGCGGCGGTGGAGGTGATGCTCGGGACCCACACGGTGCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGCACCCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGTCAGATAGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGCGACCTCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.514

94.709

0.497
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

89.683

0.381
  pilT Vibrio cholerae strain A1552

40.456

92.857

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.857

0.376
  pilT Legionella pneumophila strain ERS1305867

42.216

88.36

0.373
  pilT Legionella pneumophila strain Lp02

42.216

88.36

0.373
  pilT Pseudomonas aeruginosa PAK

40.988

91.005

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.317

88.36

0.365
  pilT Acinetobacter nosocomialis M2

41.317

88.36

0.365
  pilT Acinetobacter baumannii D1279779

41.317

88.36

0.365

Multiple sequence alignment