Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7K57_RS12480 Genome accession   NZ_AP022214
Coordinates   2690258..2691394 (+) Length   378 a.a.
NCBI ID   WP_029314740.1    Uniprot ID   -
Organism   Aeromonas caviae strain WP8-S17-ESBL-03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2685258..2696394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K57_RS12450 (WP8S17E03_23920) - 2685360..2685740 (-) 381 WP_010675195.1 diacylglycerol kinase -
  H7K57_RS12455 (WP8S17E03_23930) - 2685882..2686946 (+) 1065 WP_010675196.1 3-deoxy-7-phosphoheptulonate synthase -
  H7K57_RS12460 (WP8S17E03_23940) - 2687158..2687931 (-) 774 WP_010675197.1 ATP-binding cassette domain-containing protein -
  H7K57_RS12465 (WP8S17E03_23950) btuC 2687918..2688925 (-) 1008 WP_010675198.1 vitamin B12 ABC transporter permease BtuC -
  H7K57_RS12470 (WP8S17E03_23960) - 2689001..2689732 (-) 732 WP_010675199.1 substrate-binding periplasmic protein -
  H7K57_RS12475 (WP8S17E03_23970) - 2689845..2690201 (+) 357 WP_010675200.1 DMT family protein -
  H7K57_RS12480 (WP8S17E03_23980) pilU 2690258..2691394 (+) 1137 WP_029314740.1 type IVa pilus ATPase TapW Machinery gene
  H7K57_RS12485 (WP8S17E03_23990) - 2691456..2691698 (-) 243 WP_010675202.1 FeoC-like transcriptional regulator -
  H7K57_RS12490 (WP8S17E03_24000) feoB 2691745..2694015 (-) 2271 WP_010675203.1 Fe(2+) transporter permease subunit FeoB -
  H7K57_RS12495 (WP8S17E03_24010) - 2694012..2694239 (-) 228 WP_010675204.1 FeoA family protein -
  H7K57_RS12500 (WP8S17E03_24020) - 2694359..2694688 (+) 330 WP_010675205.1 translation initiation factor -
  H7K57_RS12505 (WP8S17E03_24030) - 2694942..2695499 (+) 558 WP_010675206.1 TMEM165/GDT1 family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42203.27 Da        Isoelectric Point: 6.6000

>NTDB_id=77184 H7K57_RS12480 WP_029314740.1 2690258..2691394(+) (pilU) [Aeromonas caviae strain WP8-S17-ESBL-03]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLGPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=77184 H7K57_RS12480 WP_029314740.1 2690258..2691394(+) (pilU) [Aeromonas caviae strain WP8-S17-ESBL-03]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCCTTGCGCCCCCTCACCCAGGAGCCCCTGGGGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCTCCGGTGCTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAATCCACATCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGTGAAATCCGCGATCGGGAGACCATGGAGCATG
CCCTCGCCTTCTCGGAAACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAACTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGGCGCCGGGCGGCGGTGGAGGTAATGCTAGGAACCCACACGGTGCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGCACCCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGCCAGATTGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGCGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.514

94.709

0.497
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

89.683

0.381
  pilT Vibrio cholerae strain A1552

40.456

92.857

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.857

0.376
  pilT Legionella pneumophila strain ERS1305867

42.216

88.36

0.373
  pilT Legionella pneumophila strain Lp02

42.216

88.36

0.373
  pilT Pseudomonas aeruginosa PAK

40.988

91.005

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.317

88.36

0.365
  pilT Acinetobacter nosocomialis M2

41.317

88.36

0.365
  pilT Acinetobacter baumannii D1279779

41.317

88.36

0.365

Multiple sequence alignment