Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   O1450_RS04055 Genome accession   NZ_CP114256
Coordinates   835390..836508 (-) Length   372 a.a.
NCBI ID   WP_004663427.1    Uniprot ID   N9LTH9
Organism   Acinetobacter sp. TR11     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 799711..866066 835390..836508 within 0


Gene organization within MGE regions


Location: 799711..866066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1450_RS03880 (O1450_03880) - 800640..800810 (+) 171 WP_269229762.1 hypothetical protein -
  O1450_RS03885 (O1450_03885) - 800866..801384 (-) 519 WP_269229763.1 hypothetical protein -
  O1450_RS03890 (O1450_03890) hemJ 801603..802055 (-) 453 WP_269229764.1 protoporphyrinogen oxidase HemJ -
  O1450_RS03895 (O1450_03895) - 802074..803303 (-) 1230 WP_269229765.1 beta-ketoacyl synthase N-terminal-like domain-containing protein -
  O1450_RS03900 (O1450_03900) - 803593..804942 (+) 1350 WP_269229766.1 EcsC family protein -
  O1450_RS03905 (O1450_03905) rpsL 805114..805488 (+) 375 WP_004638184.1 30S ribosomal protein S12 -
  O1450_RS03910 (O1450_03910) rpsG 805649..806119 (+) 471 WP_005190966.1 30S ribosomal protein S7 -
  O1450_RS03915 (O1450_03915) fusA 806297..808435 (+) 2139 WP_050041360.1 elongation factor G -
  O1450_RS03920 (O1450_03920) tuf 808529..809719 (+) 1191 WP_269229021.1 elongation factor Tu -
  O1450_RS03925 (O1450_03925) - 809922..810803 (+) 882 WP_269229767.1 metal-dependent hydrolase -
  O1450_RS03930 (O1450_03930) - 811026..811910 (+) 885 WP_269229768.1 metal-dependent hydrolase -
  O1450_RS03935 (O1450_03935) rimI 812004..812465 (+) 462 WP_269229769.1 ribosomal protein S18-alanine N-acetyltransferase -
  O1450_RS03940 (O1450_03940) - 812451..813266 (-) 816 WP_269229770.1 arginyltransferase -
  O1450_RS03945 (O1450_03945) aat 813293..814024 (-) 732 WP_269230411.1 leucyl/phenylalanyl-tRNA--protein transferase -
  O1450_RS03950 (O1450_03950) trxB 814118..815068 (-) 951 WP_018679648.1 thioredoxin-disulfide reductase -
  O1450_RS03955 (O1450_03955) - 815350..818436 (+) 3087 WP_269229771.1 DNA translocase FtsK 4TM domain-containing protein -
  O1450_RS03960 (O1450_03960) rhtC 818565..819182 (+) 618 WP_004663452.1 threonine export protein RhtC -
  O1450_RS03965 (O1450_03965) - 819226..819513 (-) 288 WP_005158274.1 PA4642 family protein -
  O1450_RS03970 (O1450_03970) minE 819639..819911 (-) 273 WP_004663449.1 cell division topological specificity factor MinE -
  O1450_RS03975 (O1450_03975) minD 819914..820726 (-) 813 WP_269229772.1 septum site-determining protein MinD -
  O1450_RS03980 (O1450_03980) minC 820792..821511 (-) 720 WP_005217839.1 septum site-determining protein MinC -
  O1450_RS03985 (O1450_03985) - 821630..822688 (-) 1059 WP_269229773.1 preprotein translocase subunit SecA -
  O1450_RS03990 (O1450_03990) - 822713..823630 (-) 918 WP_269229774.1 acyltransferase -
  O1450_RS03995 (O1450_03995) - 824020..824682 (+) 663 WP_269229775.1 OmpA family protein -
  O1450_RS04000 (O1450_04000) rep 824866..826905 (+) 2040 WP_269229776.1 DNA helicase Rep -
  O1450_RS04005 (O1450_04005) dut 826929..827381 (+) 453 WP_005158245.1 dUTP diphosphatase -
  O1450_RS04010 (O1450_04010) - 827574..828995 (+) 1422 WP_269229777.1 phosphomannomutase/phosphoglucomutase -
  O1450_RS04015 (O1450_04015) argB 829009..829908 (+) 900 WP_269229778.1 acetylglutamate kinase -
  O1450_RS04020 (O1450_04020) - 830184..830960 (+) 777 WP_269229779.1 GNAT family N-acetyltransferase -
  O1450_RS04025 (O1450_04025) - 831058..831906 (+) 849 WP_269229780.1 class II glutamine amidotransferase -
  O1450_RS04030 (O1450_04030) - 832183..832638 (+) 456 WP_269229781.1 bacteriohemerythrin -
  O1450_RS04035 (O1450_04035) - 832887..833969 (+) 1083 WP_269229782.1 hypothetical protein -
  O1450_RS04040 (O1450_04040) - 833969..834289 (+) 321 WP_269229783.1 RnfH family protein -
  O1450_RS04045 (O1450_04045) bamE 834348..834746 (-) 399 WP_269229784.1 outer membrane protein assembly factor BamE -
  O1450_RS04050 (O1450_04050) fur 834858..835295 (+) 438 WP_004663428.1 ferric iron uptake transcriptional regulator -
  O1450_RS04055 (O1450_04055) pilU 835390..836508 (-) 1119 WP_004663427.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O1450_RS04060 (O1450_04060) pilT 836533..837570 (-) 1038 WP_269229785.1 type IV pilus twitching motility protein PilT Machinery gene
  O1450_RS04065 (O1450_04065) - 837695..838387 (+) 693 WP_269229786.1 YggS family pyridoxal phosphate-dependent enzyme -
  O1450_RS04070 (O1450_04070) - 838571..839296 (+) 726 WP_269229787.1 hypothetical protein -
  O1450_RS04075 (O1450_04075) - 839337..842930 (-) 3594 WP_269229788.1 AAA family ATPase -
  O1450_RS04080 (O1450_04080) - 842940..844190 (-) 1251 WP_269229789.1 exonuclease SbcCD subunit D -
  O1450_RS04085 (O1450_04085) - 844354..845136 (+) 783 WP_269229790.1 crotonase/enoyl-CoA hydratase family protein -
  O1450_RS04090 (O1450_04090) - 845190..845576 (-) 387 WP_269229791.1 SMI1/KNR4 family protein -
  O1450_RS04095 (O1450_04095) - 845583..846236 (-) 654 WP_269229792.1 hypothetical protein -
  O1450_RS04100 (O1450_04100) - 846850..847299 (+) 450 WP_269229793.1 ABZJ_00895 family protein -
  O1450_RS04105 (O1450_04105) - 847372..848277 (+) 906 WP_269229794.1 TIGR01777 family oxidoreductase -
  O1450_RS04110 (O1450_04110) thiO 848263..849384 (-) 1122 WP_269229795.1 glycine oxidase ThiO -
  O1450_RS04115 (O1450_04115) hemB 849464..850477 (-) 1014 WP_269229796.1 porphobilinogen synthase -
  O1450_RS04120 (O1450_04120) - 850649..851146 (+) 498 WP_004663409.1 thioesterase family protein -
  O1450_RS04125 (O1450_04125) - 851413..852561 (+) 1149 WP_269229797.1 HlyD family efflux transporter periplasmic adaptor subunit -
  O1450_RS04130 (O1450_04130) - 852568..854094 (+) 1527 WP_269229798.1 DHA2 family efflux MFS transporter permease subunit -
  O1450_RS04135 (O1450_04135) - 854559..855962 (-) 1404 WP_269229799.1 P-loop NTPase fold protein -
  O1450_RS04140 (O1450_04140) - 856137..857018 (-) 882 WP_269229800.1 hypothetical protein -
  O1450_RS04145 (O1450_04145) - 857194..858345 (-) 1152 WP_269229801.1 hypothetical protein -
  O1450_RS04150 (O1450_04150) - 858419..858673 (-) 255 WP_104492180.1 hypothetical protein -
  O1450_RS04155 (O1450_04155) - 858913..859107 (-) 195 WP_269229802.1 transcriptional regulator -
  O1450_RS04160 (O1450_04160) - 859202..859960 (-) 759 WP_269229803.1 hypothetical protein -
  O1450_RS04165 (O1450_04165) - 859980..861212 (-) 1233 WP_269229804.1 tyrosine-type recombinase/integrase -
  O1450_RS04175 (O1450_04175) - 861968..862206 (-) 239 Protein_795 RND transporter -
  O1450_RS04180 (O1450_04180) - 862735..863358 (+) 624 WP_269229805.1 MarC family protein -
  O1450_RS04185 (O1450_04185) - 863579..864100 (+) 522 WP_269230412.1 acyl-CoA thioesterase -
  O1450_RS04190 (O1450_04190) - 864234..864449 (-) 216 WP_269229806.1 hypothetical protein -
  O1450_RS04195 (O1450_04195) - 864927..865940 (+) 1014 WP_269229807.1 GTP-binding protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41655.82 Da        Isoelectric Point: 6.4346

>NTDB_id=765249 O1450_RS04055 WP_004663427.1 835390..836508(-) (pilU) [Acinetobacter sp. TR11]
MDFNDLLNLMIQQSASDLFVTADVEPSMKINGQIVPVSKSKLSGEVVGQLLHSIMSDKQRKEFAETRECNFAIVNREKTA
RFRVSAFQQRDMPGMVLRRIETVIPTMDELKLPSVLKELAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHPQLHMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGEITYKDALKHADSPNDLRLTIKLSEEGTDQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=765249 O1450_RS04055 WP_004663427.1 835390..836508(-) (pilU) [Acinetobacter sp. TR11]
ATGGACTTTAATGATTTACTCAATTTAATGATTCAACAAAGTGCATCTGATTTATTTGTCACTGCAGATGTTGAACCCTC
AATGAAGATCAACGGTCAAATTGTGCCTGTTTCAAAATCTAAACTCTCAGGTGAAGTCGTTGGTCAGTTACTTCATTCAA
TTATGTCAGATAAGCAGCGTAAAGAATTTGCTGAAACACGTGAATGCAACTTTGCGATTGTCAATCGTGAAAAAACGGCA
CGTTTCCGTGTGAGTGCTTTTCAACAGCGTGATATGCCTGGGATGGTTTTACGTCGTATTGAGACTGTCATTCCAACCAT
GGATGAACTCAAGCTCCCTAGCGTGCTGAAAGAACTTGCCATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACTG
GTACAGGTAAATCAACCTCATTAGCTTCGATGATTGGTTACCGTAACCAAAACTCAAAGGGTCATATCATTACCATTGAA
GACCCGATTGAATTTGTACATCAACATGCGGGTTGTATTGTCACTCAACGTGAAGTTGGGATTGATACAGATTCATTTGA
AGTCGCATTAAAAAATACGCTTCGCCAAGCACCTGATGTGATCTTAATTGGTGAGATTCGTTCTCGTGAAACCATGGACT
ATGCAATTGCTTTTGCTGAAACTGGTCACTTAGTGCTCGCAACACTACACGCAAACAACGCGAACCAAGCACTTGATCGT
ATTATTCACTTCTTTGAAGCAGATCGTCATCCTCAATTGCATATGGATTTATCACTCAATCTGAAAGCGATGGTTGCTCA
ACAGTTGATTCCAACGCCAGATGGTAATTCACGTCGCGCAGCAATTGAGATTCTGATTAATACACCACTTTTGGCAGACT
ACATACGCAAAGGTGAAGTACACGAAATCAAAGATCTCATGAAGCGTTCTCGAGAATTGGGGATGCAGACTTTTGACCAA
GCATTATTTGATTTATACAAAGCAGGTGAAATTACGTATAAAGATGCACTTAAACATGCTGACTCACCTAATGACTTACG
TCTCACAATTAAACTTTCAGAAGAAGGTACAGATCAGTTATTGAGTGCGAGCCGAAATATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LTH9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.661

100

0.769
  pilU Pseudomonas stutzeri DSM 10701

71.429

99.731

0.712
  pilU Vibrio cholerae strain A1552

55.676

99.462

0.554
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.209

97.849

0.403
  pilT Pseudomonas aeruginosa PAK

40.173

93.011

0.374
  pilT Neisseria gonorrhoeae MS11

40

91.398

0.366
  pilT Neisseria meningitidis 8013

39.766

91.935

0.366
  pilT Acinetobacter baumannii D1279779

39.359

92.204

0.363
  pilT Acinetobacter baumannii strain A118

39.359

92.204

0.363
  pilT Acinetobacter nosocomialis M2

39.359

92.204

0.363
  pilT Pseudomonas stutzeri DSM 10701

39.017

93.011

0.363
  pilT Legionella pneumophila strain Lp02

41.284

87.903

0.363
  pilT Legionella pneumophila strain ERS1305867

41.284

87.903

0.363