Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7R62_RS14355 Genome accession   NZ_AP022038
Coordinates   3105897..3107036 (+) Length   379 a.a.
NCBI ID   WP_042057762.1    Uniprot ID   A0A6S5C636
Organism   Aeromonas veronii strain WP3-W19-ESBL-03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3100897..3112036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R62_RS14325 (WP3W19E03_27750) - 3100917..3101297 (-) 381 WP_075112066.1 diacylglycerol kinase -
  H7R62_RS14330 (WP3W19E03_27760) - 3101442..3102509 (+) 1068 WP_111806688.1 3-deoxy-7-phosphoheptulonate synthase -
  H7R62_RS14335 (WP3W19E03_27770) - 3102667..3103464 (-) 798 WP_182939764.1 ATP-binding cassette domain-containing protein -
  H7R62_RS14340 (WP3W19E03_27780) btuC 3103530..3104537 (-) 1008 WP_182937770.1 vitamin B12 ABC transporter permease BtuC -
  H7R62_RS14345 (WP3W19E03_27790) - 3104637..3105368 (-) 732 WP_182937771.1 substrate-binding periplasmic protein -
  H7R62_RS14350 (WP3W19E03_27800) - 3105482..3105838 (+) 357 WP_075112061.1 DMT family protein -
  H7R62_RS14355 (WP3W19E03_27810) pilU 3105897..3107036 (+) 1140 WP_042057762.1 type IVa pilus ATPase TapW Machinery gene
  H7R62_RS14360 (WP3W19E03_27820) - 3107133..3107582 (-) 450 WP_182937772.1 methyltransferase family protein -
  H7R62_RS14365 (WP3W19E03_27830) alr 3107756..3108982 (+) 1227 WP_075112059.1 alanine racemase -
  H7R62_RS14370 (WP3W19E03_27840) - 3109047..3109991 (-) 945 WP_005353134.1 TerC family protein -
  H7R62_RS14375 (WP3W19E03_27850) oppF 3110295..3111290 (-) 996 WP_042057772.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42280.42 Da        Isoelectric Point: 6.4293

>NTDB_id=76362 H7R62_RS14355 WP_042057762.1 3105897..3107036(+) (pilU) [Aeromonas veronii strain WP3-W19-ESBL-03]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKLQFDKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDMF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=76362 H7R62_RS14355 WP_042057762.1 3105897..3107036(+) (pilU) [Aeromonas veronii strain WP3-W19-ESBL-03]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCCCCTCCCTG
CGCCAAGTTCAACGGCGGCCTGCGCCCCCTGAATGAAACGCCGCTGGAGCCGGGAGAGGTGGCACGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAACTGCAGTTTGACAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAAATCCCCCGTTTTGA
AGACCTGAAACTTCCGCCCGTGCTGCTCGACACCATCATGGAGAAACGGGGTCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAACGGGAGGTGGGGGTCGATACCCGCAGCTTCCACGC
TGCCCTGAAAAACACCCTGCGTCAGGCACCGGATGTGATCCTGATCGGCGAAATTCGCGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCGCAGTTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAAACTACCGATGGCGGCCGCCGGGCCGCGGTGGAGATCATGCTCGGCACCCACACCATCCGTGACATGATCA
AGCGCGGCGAATTTGGCGGCCTGAAAGAGGTGATGGAAAAATCCAAGGCGCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGAGGAGGAGGAAGCAGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAAGAGAAGGGACAGATTGCCAGCAGCAGCGATGCCACTGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGACGATATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S5C636

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.211

100

0.544
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

89.446

0.372
  pilT Pseudomonas aeruginosa PAK

40.988

90.765

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii D1279779

41.317

88.127

0.364
  pilT Acinetobacter baumannii strain A118

41.317

88.127

0.364
  pilT Acinetobacter nosocomialis M2

41.317

88.127

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364

Multiple sequence alignment