Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OOK74_RS09340 Genome accession   NZ_CP113241
Coordinates   2048396..2048836 (+) Length   146 a.a.
NCBI ID   WP_278182670.1    Uniprot ID   -
Organism   Vibrio sp. gvc     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2043396..2053836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOK74_RS09315 dsbC 2043954..2044715 (-) 762 WP_278182667.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  OOK74_RS09320 xerD 2044732..2045610 (-) 879 WP_274722896.1 site-specific tyrosine recombinase XerD -
  OOK74_RS09325 fldB 2045886..2046404 (+) 519 WP_274722832.1 flavodoxin FldB -
  OOK74_RS09330 ampD 2046464..2047018 (-) 555 WP_278182668.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OOK74_RS09335 nadC 2047286..2048191 (+) 906 WP_278182669.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OOK74_RS09340 pilA 2048396..2048836 (+) 441 WP_278182670.1 pilin Machinery gene
  OOK74_RS09345 pilB 2048843..2050531 (+) 1689 WP_278182671.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OOK74_RS09350 pilC 2050584..2051810 (+) 1227 WP_278182672.1 type II secretion system F family protein Machinery gene
  OOK74_RS09355 pilD 2051865..2052737 (+) 873 WP_278182673.1 A24 family peptidase Machinery gene
  OOK74_RS09360 coaE 2052734..2053342 (+) 609 WP_278182674.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15148.24 Da        Isoelectric Point: 6.9450

>NTDB_id=761107 OOK74_RS09340 WP_278182670.1 2048396..2048836(+) (pilA) [Vibrio sp. gvc]
MKAYNKLQQGFTLIELMIVVAIIGILAAIAVPQYQKYVAKSEAAAALASITAHRTNVEMYVVENGAFPQDTNDLNIPSTT
SGEISYGQAASGAAGTGSGTIIFTFAASGASPSVVSKLVTLQRDGNGTWTCSSTIEANDLKPKGCK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=761107 OOK74_RS09340 WP_278182670.1 2048396..2048836(+) (pilA) [Vibrio sp. gvc]
ATGAAAGCGTATAACAAACTGCAACAGGGCTTTACCCTGATTGAATTAATGATTGTGGTAGCAATTATTGGCATTTTGGC
CGCAATTGCCGTACCACAATATCAGAAATACGTTGCCAAAAGTGAAGCGGCAGCAGCACTGGCTTCAATTACCGCTCATA
GAACCAATGTGGAAATGTACGTGGTGGAAAATGGGGCTTTTCCTCAGGATACCAATGATCTGAATATTCCATCCACAACC
TCTGGGGAAATAAGTTATGGGCAAGCAGCATCTGGGGCTGCTGGGACAGGCTCAGGGACTATCATCTTTACATTCGCTGC
CAGCGGGGCAAGTCCAAGTGTAGTAAGTAAACTTGTCACATTACAACGTGATGGAAATGGTACGTGGACGTGTAGTTCAA
CAATTGAAGCCAATGATTTAAAACCTAAAGGTTGTAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(39-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

49.351

100

0.521
  pilA Vibrio cholerae C6706

49.351

100

0.521
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

49.351

100

0.521
  pilA Pseudomonas aeruginosa PAK

43.919

100

0.445
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.333

100

0.445
  pilA Vibrio parahaemolyticus RIMD 2210633

41.667

98.63

0.411
  pilA Acinetobacter baumannii strain A118

43.478

94.521

0.411
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.255

100

0.39
  pilA Ralstonia pseudosolanacearum GMI1000

37.589

96.575

0.363
  pilA2 Legionella pneumophila str. Paris

37.589

96.575

0.363
  pilA2 Legionella pneumophila strain ERS1305867

37.589

96.575

0.363