Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OOK74_RS09345 Genome accession   NZ_CP113241
Coordinates   2048843..2050531 (+) Length   562 a.a.
NCBI ID   WP_278182671.1    Uniprot ID   -
Organism   Vibrio sp. gvc     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2043843..2055531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOK74_RS09315 dsbC 2043954..2044715 (-) 762 WP_278182667.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  OOK74_RS09320 xerD 2044732..2045610 (-) 879 WP_274722896.1 site-specific tyrosine recombinase XerD -
  OOK74_RS09325 fldB 2045886..2046404 (+) 519 WP_274722832.1 flavodoxin FldB -
  OOK74_RS09330 ampD 2046464..2047018 (-) 555 WP_278182668.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OOK74_RS09335 nadC 2047286..2048191 (+) 906 WP_278182669.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OOK74_RS09340 pilA 2048396..2048836 (+) 441 WP_278182670.1 pilin Machinery gene
  OOK74_RS09345 pilB 2048843..2050531 (+) 1689 WP_278182671.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OOK74_RS09350 pilC 2050584..2051810 (+) 1227 WP_278182672.1 type II secretion system F family protein Machinery gene
  OOK74_RS09355 pilD 2051865..2052737 (+) 873 WP_278182673.1 A24 family peptidase Machinery gene
  OOK74_RS09360 coaE 2052734..2053342 (+) 609 WP_278182674.1 dephospho-CoA kinase -
  OOK74_RS09365 zapD 2053374..2054114 (+) 741 WP_274722840.1 cell division protein ZapD -
  OOK74_RS09370 yacG 2054199..2054396 (+) 198 WP_277206628.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62011.12 Da        Isoelectric Point: 5.8947

>NTDB_id=761108 OOK74_RS09345 WP_278182671.1 2048843..2050531(+) (pilB) [Vibrio sp. gvc]
MLTNLIAILRQAELISAAQQQAVLEHVSAADTSVPEALLELKIFHPQQLTEQLSHIFGLPETDLNRYDYAELCQQLGLRE
LITRYQALPLAKQGHVLLLAVSDPTLLQAEEEFRFASGLQVELALANHRALQAAIRRLYGRSIQGAASQGKEISQDELAD
LVQVGADELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQFRIRLRCDGILVETQQPASQLSRRLAARLKI
LAKLDIAERRLPQDGRIKLRLNADTAIDLRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLTALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTAQINISTAEDPVEINLTGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKQCKVAHAPSAILQNQFAFQAQE
VLYQANPAGCNECTGGYSGRVGIYEVMAFNPELAEAIMQRASIQQIEQLASANGMQTLQQSGLEKLRQGITSFTELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=761108 OOK74_RS09345 WP_278182671.1 2048843..2050531(+) (pilB) [Vibrio sp. gvc]
ATGCTCACCAATCTAATCGCCATCTTGCGCCAAGCGGAGTTAATTAGCGCGGCACAACAGCAAGCGGTGCTAGAACACGT
TAGCGCTGCCGATACTTCGGTGCCGGAAGCGCTGCTGGAGCTAAAGATTTTCCACCCTCAGCAGTTAACCGAGCAACTGA
GCCATATTTTTGGCTTGCCGGAAACCGATCTTAACCGCTACGACTATGCCGAGCTGTGCCAACAGCTCGGTTTGCGTGAA
CTAATTACCCGCTATCAAGCACTGCCACTGGCCAAACAAGGCCATGTGCTACTGCTGGCGGTGTCCGATCCAACCTTACT
GCAAGCAGAAGAAGAGTTTCGCTTCGCCTCTGGGCTACAAGTTGAGTTGGCACTGGCCAATCATCGCGCCTTGCAAGCGG
CAATCCGCCGTTTGTATGGTCGGTCAATTCAAGGGGCGGCGAGTCAAGGCAAAGAGATCAGTCAAGATGAGCTGGCCGAT
CTGGTGCAAGTCGGTGCCGATGAGCTGCAATCGATTGAAGATTTAAGCCAAGATGACTCGCCGGTCAGCCGCTTTATCAA
CCAAGTGCTGCTTGATGCAGTGCGTAAAGGCGCCTCCGACATCCATTTTGAACCTTATGAAAACCAATTTCGCATTCGGC
TACGCTGCGATGGGATTTTGGTCGAAACTCAACAACCGGCTAGCCAGCTCAGCCGCCGCTTAGCCGCGCGGCTAAAAATC
CTTGCCAAACTGGATATTGCCGAACGCCGCCTGCCGCAAGATGGGCGGATCAAACTGCGCCTCAATGCCGATACCGCGAT
TGATCTGCGTGTCTCTACCTTGCCCACGTTATGGGGTGAAAAAATTGTTCTGCGTCTGCTCGACAGCAGCGCGGCCAATT
TGGATATTGATAAGCTCGGCTACAACCCGCAGCAAAAACAGCTCTATTTAACCGCACTAAAACGGCCACAAGGGATGATT
TTAATGACTGGCCCAACCGGCAGCGGTAAAACCGTATCGCTGTACACAGGGCTGCGCATTCTTAATACCGCGCAAATCAA
TATCTCCACCGCGGAAGATCCGGTCGAAATTAACCTAACGGGTATTAACCAAGTACAAGTGCAACCGAAAATTGGTTTTG
GTTTTGCCGAAGCGCTGCGCTCGTTTCTGCGCCAAGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATCTGGAAACC
GCAGAAATCGCGGTCAAAGCGGCGCAAACCGGTCACTTAGTACTCTCGACTTTACACACCAATTCCGCGGCAGAAACCGT
GATCCGCTTAGCCAATATGGGGGTGGAGCCGTTTAACCTAGCCTCATCACTGAGTTTAATTATTGCCCAGCGGCTAGCGC
GTCGCTTATGCAAACAGTGCAAAGTGGCTCATGCGCCATCAGCCATTTTGCAAAACCAGTTTGCCTTTCAAGCACAAGAA
GTGTTGTATCAAGCCAATCCGGCTGGCTGCAATGAATGTACCGGTGGCTATTCTGGTCGGGTCGGCATTTATGAGGTGAT
GGCGTTTAATCCAGAGCTGGCAGAAGCGATTATGCAGCGCGCCTCCATTCAACAAATTGAACAACTAGCCAGCGCAAACG
GCATGCAAACCCTGCAACAGTCCGGCTTAGAAAAACTGCGCCAAGGCATCACCAGTTTTACCGAGCTACAACGGGTACTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

90.747

100

0.907
  pilB Vibrio campbellii strain DS40M4

72.954

100

0.73
  pilB Vibrio parahaemolyticus RIMD 2210633

72.42

100

0.724
  pilB Acinetobacter baumannii D1279779

49.47

100

0.498
  pilB Legionella pneumophila strain ERS1305867

49.725

96.975

0.482
  pilB Acinetobacter baylyi ADP1

49.18

97.687

0.48
  pilF Neisseria gonorrhoeae MS11

45.035

100

0.452
  pilB/pilB1 Synechocystis sp. PCC 6803

38.103

100

0.407
  pilB Haemophilus influenzae 86-028NP

40.114

93.594

0.375
  pilF Thermus thermophilus HB27

39.469

93.772

0.37
  pilB Haemophilus influenzae Rd KW20

39.847

92.883

0.37