Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   OOK74_RS09350 Genome accession   NZ_CP113241
Coordinates   2050584..2051810 (+) Length   408 a.a.
NCBI ID   WP_278182672.1    Uniprot ID   -
Organism   Vibrio sp. gvc     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2045584..2056810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOK74_RS09325 fldB 2045886..2046404 (+) 519 WP_274722832.1 flavodoxin FldB -
  OOK74_RS09330 ampD 2046464..2047018 (-) 555 WP_278182668.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OOK74_RS09335 nadC 2047286..2048191 (+) 906 WP_278182669.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OOK74_RS09340 pilA 2048396..2048836 (+) 441 WP_278182670.1 pilin Machinery gene
  OOK74_RS09345 pilB 2048843..2050531 (+) 1689 WP_278182671.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OOK74_RS09350 pilC 2050584..2051810 (+) 1227 WP_278182672.1 type II secretion system F family protein Machinery gene
  OOK74_RS09355 pilD 2051865..2052737 (+) 873 WP_278182673.1 A24 family peptidase Machinery gene
  OOK74_RS09360 coaE 2052734..2053342 (+) 609 WP_278182674.1 dephospho-CoA kinase -
  OOK74_RS09365 zapD 2053374..2054114 (+) 741 WP_274722840.1 cell division protein ZapD -
  OOK74_RS09370 yacG 2054199..2054396 (+) 198 WP_277206628.1 DNA gyrase inhibitor YacG -
  OOK74_RS09375 parC 2054503..2056785 (-) 2283 WP_278182675.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44630.77 Da        Isoelectric Point: 10.4028

>NTDB_id=761109 OOK74_RS09350 WP_278182672.1 2050584..2051810(+) (pilC) [Vibrio sp. gvc]
MQASKSVALKHYRWKGINSNGKKVHGQLLALSELEVRDKLKEQHIQIKKLKKGSVSLLSRLTHRVKGKDITLLTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKGVEAGTPLSKSMRTASAHFDSLYVDLVATGEMSGNLPDVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFEAMFSGFGAELPWFTQQVLKLSHWVQAYSGWALLAMGAALFG
LKTLRQKSLAVRLATSRLSLKIPVIGSVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTAGNIHFEHAINQVHRDTAA
GVPMYIALRNTEAFPEMVLQMVMIGEESGQLDDMLNKVASIYEFEVDSTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=761109 OOK74_RS09350 WP_278182672.1 2050584..2051810(+) (pilC) [Vibrio sp. gvc]
ATGCAAGCGAGCAAATCTGTAGCCTTAAAACACTACCGCTGGAAAGGCATCAACAGTAACGGCAAAAAAGTCCACGGCCA
GTTACTGGCACTCAGCGAATTAGAAGTGCGCGACAAACTCAAAGAGCAGCACATTCAGATCAAAAAACTCAAAAAAGGCA
GCGTCTCCTTGCTGTCTCGCCTCACTCATCGGGTCAAAGGTAAAGACATCACCCTATTAACCCGCCAACTGGCCACCATG
CTCACCACTGGCGTACCGATTGTGCAAGCGCTCAAGTTGGTAGGCGATAACCATCGCAAAGCGGAGATGAAATCGATCCT
AGCGCAAATCACCAAAGGGGTTGAAGCGGGTACGCCACTCTCAAAATCGATGCGTACCGCCAGCGCGCATTTTGACAGCT
TGTATGTTGATTTGGTGGCAACCGGTGAAATGTCCGGCAATTTGCCGGATGTGTTTGAACGCTTGGCGACTTATCGGGAA
AAAAGTGAACAACTGCGCGCTAAAGTGATCAAAGCGCTGATTTACCCCAGCATGGTGGTGTTAGTGGCACTTGGAGTTTC
TTACCTAATGCTGACTATGGTGATCCCCGAATTTGAAGCAATGTTTAGCGGTTTTGGGGCGGAACTACCTTGGTTTACTC
AGCAAGTGCTCAAACTATCACACTGGGTACAAGCCTATAGTGGATGGGCATTACTGGCGATGGGAGCGGCACTCTTCGGG
CTCAAAACGCTACGCCAAAAATCATTGGCAGTGCGTTTAGCCACCAGTCGCCTCAGCCTGAAAATACCGGTTATCGGCTC
AGTACTGGCCAAAGCCTCGATCGCCAAATTCAGCCGCACTCTCGCCACCAGCTTTGCCGCGGGTATTCCCATCCTCGCCA
GTTTAAAAACCACTGCCAAAACCGCAGGTAACATCCACTTTGAACACGCCATCAATCAAGTACACCGCGATACGGCGGCC
GGGGTACCGATGTATATTGCGCTGCGCAATACCGAAGCGTTTCCGGAAATGGTGCTGCAAATGGTGATGATTGGCGAAGA
GTCCGGCCAGTTGGATGACATGCTAAATAAGGTCGCCAGCATCTACGAATTTGAAGTCGATAGTACCGTGGATAATTTAG
GCAAAATTCTTGAGCCGCTGATCATTGTGTTTCTTGGCACGGTAGTGGGTGGCTTAGTGGTGGCGATGTACTTGCCGATC
TTTAACTTGATGAGTGTCTTAGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

88.235

100

0.882
  pilC Vibrio campbellii strain DS40M4

72.25

98.039

0.708
  pilC Pseudomonas stutzeri DSM 10701

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

41.935

98.775

0.414
  pilC Acinetobacter baylyi ADP1

40.441

100

0.404
  pilC Acinetobacter baumannii D1279779

39.951

100

0.4
  pilG Neisseria gonorrhoeae MS11

40.299

98.529

0.397
  pilG Neisseria meningitidis 44/76-A

39.801

98.529

0.392