Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PALA30_RS26435 Genome accession   NZ_CP110345
Coordinates   5610196..5610645 (-) Length   149 a.a.
NCBI ID   WP_031636653.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5605196..5615645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA30_RS26415 (PALA30_05200) ampD 5605888..5606454 (-) 567 WP_003094640.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PALA30_RS26420 (PALA30_05201) - 5606605..5608860 (-) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  PALA30_RS26425 (PALA30_05202) nadC 5609146..5609994 (+) 849 WP_023435051.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA30_RS26435 (PALA30_05204) pilA 5610196..5610645 (-) 450 WP_031636653.1 pilin Machinery gene
  PALA30_RS26440 (PALA30_05205) pilB 5610877..5612577 (+) 1701 WP_024928731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA30_RS26445 (PALA30_05206) pilC 5612581..5613801 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  PALA30_RS26450 (PALA30_05207) pilD 5613805..5614677 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA30_RS26455 (PALA30_05208) coaE 5614674..5615285 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA30_RS26460 (PALA30_05209) yacG 5615282..5615482 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15551.89 Da        Isoelectric Point: 8.4770

>NTDB_id=754203 PALA30_RS26435 WP_031636653.1 5610196..5610645(-) (pilA) [Pseudomonas aeruginosa strain PALA30]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTDPSTATETYV
GIAANANKLGLIDVKIADTGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=754203 PALA30_RS26435 WP_031636653.1 5610196..5610645(-) (pilA) [Pseudomonas aeruginosa strain PALA30]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTTATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTGATCCATCTACTGCCACCGAAACATATGTT
GGGATTGCTGCGAATGCCAACAAGCTGGGCTTGATTGATGTAAAGATTGCTGATACCGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACTTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAATCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.667

100

0.691
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.98

100

0.47
  pilA Acinetobacter baumannii strain A118

46.622

99.329

0.463
  pilA Vibrio cholerae strain A1552

42.949

100

0.45
  pilA Vibrio cholerae C6706

42.949

100

0.45
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.949

100

0.45
  pilA Vibrio parahaemolyticus RIMD 2210633

44.898

98.658

0.443
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.455

95.973

0.436
  comP Acinetobacter baylyi ADP1

41.83

100

0.43
  pilA2 Legionella pneumophila str. Paris

38.514

99.329

0.383
  pilA2 Legionella pneumophila strain ERS1305867

37.838

99.329

0.376
  pilA Haemophilus influenzae 86-028NP

36.242

100

0.362