Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PALA30_RS26440 Genome accession   NZ_CP110345
Coordinates   5610877..5612577 (+) Length   566 a.a.
NCBI ID   WP_024928731.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA30     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5605877..5617577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA30_RS26415 (PALA30_05200) ampD 5605888..5606454 (-) 567 WP_003094640.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PALA30_RS26420 (PALA30_05201) - 5606605..5608860 (-) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  PALA30_RS26425 (PALA30_05202) nadC 5609146..5609994 (+) 849 WP_023435051.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA30_RS26435 (PALA30_05204) pilA 5610196..5610645 (-) 450 WP_031636653.1 pilin Machinery gene
  PALA30_RS26440 (PALA30_05205) pilB 5610877..5612577 (+) 1701 WP_024928731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA30_RS26445 (PALA30_05206) pilC 5612581..5613801 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  PALA30_RS26450 (PALA30_05207) pilD 5613805..5614677 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA30_RS26455 (PALA30_05208) coaE 5614674..5615285 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA30_RS26460 (PALA30_05209) yacG 5615282..5615482 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PALA30_RS26465 (PALA30_05210) - 5615519..5615728 (-) 210 WP_003094660.1 hypothetical protein -
  PALA30_RS26470 (PALA30_05211) - 5615834..5616523 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PALA30_RS26475 (PALA30_05212) - 5616520..5616990 (-) 471 WP_003094664.1 hypothetical protein -
  PALA30_RS26480 (PALA30_05213) - 5616987..5617412 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62355.22 Da        Isoelectric Point: 5.7041

>NTDB_id=754204 PALA30_RS26440 WP_024928731.1 5610877..5612577(+) (pilB) [Pseudomonas aeruginosa strain PALA30]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=754204 PALA30_RS26440 WP_024928731.1 5610877..5612577(+) (pilB) [Pseudomonas aeruginosa strain PALA30]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAAGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACACACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAGAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGCAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCGCCTGTAGTACGTTT
CGTAAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGAG
TGCGCTTCCGTACCGACGGCATGCTTCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAAGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.517

100

0.581
  pilB Acinetobacter baylyi ADP1

57.194

99.47

0.569
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

50.887

99.647

0.507
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.645

99.647

0.495
  pilF Thermus thermophilus HB27

39.572

99.117

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

93.11

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366