Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PALA33_RS26145 Genome accession   NZ_CP109845
Coordinates   5559313..5559765 (-) Length   150 a.a.
NCBI ID   WP_024947914.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5554313..5564765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA33_RS26125 (PALA33_05179) ampD 5555019..5555585 (-) 567 WP_023084738.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PALA33_RS26130 (PALA33_05180) - 5555736..5557991 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  PALA33_RS26135 (PALA33_05181) nadC 5558277..5559125 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA33_RS26145 pilA 5559313..5559765 (-) 453 WP_024947914.1 pilin Machinery gene
  PALA33_RS26150 (PALA33_05183) pilB 5559994..5561694 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA33_RS26155 (PALA33_05184) pilC 5561698..5562918 (+) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  PALA33_RS26160 (PALA33_05185) pilD 5562922..5563794 (+) 873 WP_023087804.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA33_RS26165 (PALA33_05186) coaE 5563791..5564402 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PALA33_RS26170 (PALA33_05187) yacG 5564399..5564599 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15622.02 Da        Isoelectric Point: 8.4827

>NTDB_id=750029 PALA33_RS26145 WP_024947914.1 5559313..5559765(-) (pilA) [Pseudomonas aeruginosa strain PALA33]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG
VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=750029 PALA33_RS26145 WP_024947914.1 5559313..5559765(-) (pilA) [Pseudomonas aeruginosa strain PALA33]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT
TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG
AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG
GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC
TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA
AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

100

100

1
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.682

100

0.48
  pilA Acinetobacter baumannii strain A118

45.333

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.575

97.333

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.675

100

0.447
  pilA Vibrio cholerae C6706

42.675

100

0.447
  pilA Vibrio cholerae strain A1552

42.675

100

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

48.507

89.333

0.433
  comP Acinetobacter baylyi ADP1

40.789

100

0.413
  pilA2 Legionella pneumophila strain ERS1305867

39.865

98.667

0.393
  pilA/pilA1 Eikenella corrodens VA1

39.456

98

0.387
  pilA2 Legionella pneumophila str. Paris

39.189

98.667

0.387