NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	P4B34_RS06480	Genome accession	NZ_CP120859
Coordinates	1344067..1344531 (+)	Length	154 a.a.
NCBI ID	WP_031630343.1	Uniprot ID	Q93PL5
Organism	Pseudomonas aeruginosa strain NY4383
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1339067..1349531

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4B34_RS06455 (P4B34_06460)	yacG	1339232..1339432 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
P4B34_RS06460 (P4B34_06465)	coaE	1339429..1340040 (-)	612	WP_003094654.1	dephospho-CoA kinase	-
P4B34_RS06465 (P4B34_06470)	pilD	1340037..1340909 (-)	873	WP_003094651.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
P4B34_RS06470 (P4B34_06475)	pilC	1340913..1342133 (-)	1221	WP_003094649.1	type II secretion system F family protein	Machinery gene
P4B34_RS06475 (P4B34_06480)	pilB	1342137..1343837 (-)	1701	WP_003094647.1	type IV-A pilus assembly ATPase PilB	Machinery gene
P4B34_RS06480 (P4B34_06485)	pilA	1344067..1344531 (+)	465	WP_031630343.1	pilin	Machinery gene
P4B34_RS06490 (P4B34_06495)	nadC	1344735..1345583 (-)	849	WP_003094645.1	carboxylating nicotinate-nucleotide diphosphorylase	-
P4B34_RS06495 (P4B34_06500)	-	1345869..1348124 (+)	2256	WP_003094643.1	DUF1631 domain-containing protein	-
P4B34_RS06500 (P4B34_06505)	ampD	1348275..1348841 (+)	567	WP_058137634.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Sequence

Protein

Download Length: 154 a.a. Molecular weight: 15780.91 Da Isoelectric Point: 6.1571

                                >NTDB_id=734769 P4B34_RS06480 WP_031630343.1 1344067..1344531(+) (pilA) [Pseudomonas aeruginosa strain NY4383]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVSRSQVSRVMAEAGSLKTAVEACLQDGRTAVGTAAGQCDPGATGSSL

LTGASQTSQTLPTNTGVPQVLDPLTTQTTIIATFGNGASAAISGQTLTWTRDVNGGWSCATTVDAKFRPNGCTD

Nucleotide

Download Length: 465 bp

                                >NTDB_id=734769 P4B34_RS06480 WP_031630343.1 1344067..1344531(+) (pilA) [Pseudomonas aeruginosa strain NY4383]

ATGAAAGCACAGAAGGGTTTTACTCTGATTGAACTGATGATCGTAGTTGCGATCATTGGTATTCTGGCTGCAATCGCAAT

TCCCCAATATCAGAACTATGTTTCTCGCTCTCAGGTCTCCAGGGTTATGGCAGAGGCTGGCTCCTTGAAGACTGCAGTTG

AGGCCTGCCTCCAGGATGGTCGTACTGCTGTGGGTACTGCTGCTGGTCAATGCGACCCGGGTGCGACGGGTTCCAGTTTG

TTGACTGGTGCTTCTCAGACTTCTCAAACCCTGCCAACCAATACCGGTGTTCCGCAGGTTCTGGATCCTCTGACTACTCA

AACCACTATCATTGCGACTTTTGGTAACGGTGCATCCGCAGCTATTTCTGGCCAGACTCTGACCTGGACTCGTGATGTTA

ATGGTGGCTGGAGCTGTGCTACTACCGTAGATGCTAAATTCCGTCCTAATGGCTGTACTGACTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-153)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q93PL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	46.753	100	0.468
pilA	Vibrio cholerae strain A1552	43.59	100	0.442
pilA	Vibrio cholerae C6706	43.59	100	0.442
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	43.59	100	0.442
comP	Acinetobacter baylyi ADP1	40.12	100	0.435
pilA	Vibrio parahaemolyticus RIMD 2210633	40.881	100	0.422
pilA	Acinetobacter baumannii strain A118	41.83	99.351	0.416
pilA2	Legionella pneumophila strain ERS1305867	41.558	100	0.416
pilA2	Legionella pneumophila str. Paris	40.909	100	0.409
pilA/pilAI	Pseudomonas stutzeri DSM 10701	40	100	0.403
pilE	Neisseria gonorrhoeae MS11	33.908	100	0.383
pilA/pilAII	Pseudomonas stutzeri DSM 10701	38.411	98.052	0.377

Detailed information NTG0734769