Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   P4B31_RS25440 Genome accession   NZ_CP120857
Coordinates   5429520..5429993 (-) Length   157 a.a.
NCBI ID   WP_309253345.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY7570     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5424520..5434993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4B31_RS25420 (P4B31_25425) ampD 5425261..5425827 (-) 567 WP_009875882.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  P4B31_RS25425 (P4B31_25430) - 5425978..5428233 (-) 2256 WP_019726810.1 DUF1631 domain-containing protein -
  P4B31_RS25430 (P4B31_25435) nadC 5428519..5429367 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  P4B31_RS25440 (P4B31_25445) pilE 5429520..5429993 (-) 474 WP_309253345.1 pilin Machinery gene
  P4B31_RS25445 (P4B31_25450) - 5430224..5430817 (+) 594 Protein_5031 type IV-A pilus assembly ATPase PilB -
  P4B31_RS25450 (P4B31_25455) - 5431003..5432562 (-) 1560 WP_256825318.1 PAS domain-containing methyl-accepting chemotaxis protein -
  P4B31_RS25455 (P4B31_25460) - 5432630..5433394 (-) 765 WP_001389365.1 IS6-like element IS6100 family transposase -
  P4B31_RS25465 (P4B31_25470) - 5433901..5434401 (-) 501 WP_000376623.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16062.43 Da        Isoelectric Point: 8.4668

>NTDB_id=734608 P4B31_RS25440 WP_309253345.1 5429520..5429993(-) (pilE) [Pseudomonas aeruginosa strain NY7570]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLKGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=734608 P4B31_RS25440 WP_309253345.1 5429520..5429993(-) (pilE) [Pseudomonas aeruginosa strain NY7570]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAAGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

42.197

100

0.465
  comP Acinetobacter baylyi ADP1

47.712

97.452

0.465
  pilE Neisseria gonorrhoeae MS11

43.114

100

0.459
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.176

100

0.446
  pilA/pilA1 Eikenella corrodens VA1

41.975

100

0.433
  pilA2 Legionella pneumophila str. Paris

45.578

93.631

0.427
  pilA2 Legionella pneumophila strain ERS1305867

44.218

93.631

0.414
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.333

95.541

0.414
  pilA Ralstonia pseudosolanacearum GMI1000

40.252

100

0.408
  pilA Pseudomonas aeruginosa PAK

42.384

96.178

0.408
  pilA Acinetobacter nosocomialis M2

55.357

71.338

0.395
  pilA Acinetobacter baumannii strain A118

37.654

100

0.389
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.5

96.815

0.363