Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EZU31_RS08555 Genome accession   NZ_AP019193
Coordinates   1806067..1807206 (-) Length   379 a.a.
NCBI ID   WP_011706550.1    Uniprot ID   A0AAI9Q2R8
Organism   Aeromonas hydrophila strain GSH8-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1801067..1812206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZU31_RS08535 (AHGSH82_016220) feoB 1801274..1803547 (+) 2274 WP_124252919.1 Fe(2+) transporter permease subunit FeoB -
  EZU31_RS08540 (AHGSH82_016230) - 1803544..1803786 (+) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  EZU31_RS08545 (AHGSH82_016240) - 1803897..1804376 (-) 480 WP_167297479.1 WbuC family cupin fold metalloprotein -
  EZU31_RS08550 (AHGSH82_016250) glgC 1804512..1805783 (-) 1272 WP_011706551.1 glucose-1-phosphate adenylyltransferase -
  EZU31_RS08555 (AHGSH82_016260) pilU 1806067..1807206 (-) 1140 WP_011706550.1 type IVa pilus ATPase TapW Machinery gene
  EZU31_RS08560 (AHGSH82_016270) - 1807267..1807623 (-) 357 WP_005302630.1 DMT family protein -
  EZU31_RS08565 (AHGSH82_016280) - 1807737..1808468 (+) 732 WP_029300033.1 substrate-binding periplasmic protein -
  EZU31_RS08570 (AHGSH82_016290) btuC 1808569..1809576 (+) 1008 WP_130632067.1 vitamin B12 ABC transporter permease BtuC -
  EZU31_RS08575 (AHGSH82_016300) - 1809563..1810348 (+) 786 WP_167297480.1 ATP-binding cassette domain-containing protein -
  EZU31_RS08580 (AHGSH82_016310) aac(6') 1810370..1810828 (-) 459 WP_130632068.1 aminoglycoside 6'-N-acetyltransferase -
  EZU31_RS08585 (AHGSH82_016320) - 1810835..1811899 (-) 1065 WP_042067836.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42264.32 Da        Isoelectric Point: 6.4290

>NTDB_id=72200 EZU31_RS08555 WP_011706550.1 1806067..1807206(-) (pilU) [Aeromonas hydrophila strain GSH8-2]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTSDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=72200 EZU31_RS08555 WP_011706550.1 1806067..1807206(-) (pilU) [Aeromonas hydrophila strain GSH8-2]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAGTGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGTCGT
TTCCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAGTCCACTTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTGCATCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
TGCCCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGTGAAATTCGCGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCGATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGCCCCCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTCAAAACCAGCGATGGTGGTCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATTCGCGACATGATCA
AACGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCGAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGACG
AGAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361

Multiple sequence alignment