Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   EL277_RS10575 Genome accession   NZ_AP018936
Coordinates   1974285..1975226 (-) Length   313 a.a.
NCBI ID   WP_000249564.1    Uniprot ID   Q8DN86
Organism   Streptococcus pneumoniae strain NU83127     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1969285..1980226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL277_RS10535 (SPNU_1990) comYH 1969771..1970724 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  EL277_RS10540 - 1970785..1971379 (-) 595 Protein_1954 class I SAM-dependent methyltransferase -
  EL277_RS10545 (SPNU_1993) comGG/cglG 1971516..1971929 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  EL277_RS10550 (SPNU_1994) comGF/cglF 1971907..1972368 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL277_RS10555 (SPNU_1995) comGE/cglE 1972331..1972633 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EL277_RS10560 (SPNU_1996) comGD/cglD 1972596..1973000 (-) 405 WP_000588028.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL277_RS10565 (SPNU_1997) comGC/cglC 1972993..1973319 (-) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  EL277_RS10570 (SPNU_1998) comGB/cglB 1973321..1974337 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL277_RS10575 (SPNU_1999) comGA/cglA/cilD 1974285..1975226 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  EL277_RS10580 (SPNU_2000) - 1975302..1975667 (-) 366 WP_000286415.1 DUF1033 family protein -
  EL277_RS10585 (SPNU_2001) - 1975818..1976876 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  EL277_RS10590 (SPNU_2002) nagA 1977039..1978190 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  EL277_RS10595 (SPNU_2003) - 1978343..1980160 (-) 1818 WP_001220853.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35555.47 Da        Isoelectric Point: 6.1330

>NTDB_id=71827 EL277_RS10575 WP_000249564.1 1974285..1975226(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain NU83127]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=71827 EL277_RS10575 WP_000249564.1 1974285..1975226(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain NU83127]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DN86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae D39

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae R6

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

100

100

1
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment