Detailed information NTG0071822

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: EL277_RS10550
• Genome accession: NZ_AP018936
• Coordinates: 1971907..1972368 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250534.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain NU83127
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1966907..1977368

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL277_RS10520 (SPNU_1987)	-	1967211..1968035 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
EL277_RS10525 (SPNU_1988)	rnpA	1968010..1968381 (-)	372	WP_000739253.1	ribonuclease P protein component	-
EL277_RS12635	-	1968398..1968529 (-)	132	WP_000768904.1	hypothetical protein	-
EL277_RS10530 (SPNU_1989)	-	1968530..1969720 (-)	1191	WP_000167757.1	acetate kinase	-
EL277_RS10535 (SPNU_1990)	comYH	1969771..1970724 (-)	954	WP_000345135.1	class I SAM-dependent methyltransferase	Machinery gene
EL277_RS10540	-	1970785..1971379 (-)	595	Protein_1954	class I SAM-dependent methyltransferase	-
EL277_RS10545 (SPNU_1993)	comGG/cglG	1971516..1971929 (-)	414	WP_000265622.1	competence type IV pilus minor pilin ComGG	Machinery gene
EL277_RS10550 (SPNU_1994)	comGF/cglF	1971907..1972368 (-)	462	WP_000250534.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL277_RS10555 (SPNU_1995)	comGE/cglE	1972331..1972633 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
EL277_RS10560 (SPNU_1996)	comGD/cglD	1972596..1973000 (-)	405	WP_000588028.1	competence type IV pilus minor pilin ComGD	Machinery gene
EL277_RS10565 (SPNU_1997)	comGC/cglC	1972993..1973319 (-)	327	WP_000738627.1	comG operon protein ComGC	Machinery gene
EL277_RS10570 (SPNU_1998)	comGB/cglB	1973321..1974337 (-)	1017	WP_074196785.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL277_RS10575 (SPNU_1999)	comGA/cglA/cilD	1974285..1975226 (-)	942	WP_000249564.1	competence type IV pilus ATPase ComGA	Machinery gene
EL277_RS10580 (SPNU_2000)	-	1975302..1975667 (-)	366	WP_000286415.1	DUF1033 family protein	-
EL277_RS10585 (SPNU_2001)	-	1975818..1976876 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17972.67 Da
• Isoelectric Point: 9.6092

>NTDB_id=71822 EL277_RS10550 WP_000250534.1 1971907..1972368(-) (comGF/cglF) [Streptococcus pneumoniae strain NU83127]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=71822 EL277_RS10550 WP_000250534.1 1971907..1972368(-) (comGF/cglF) [Streptococcus pneumoniae strain NU83127]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGACTCAAATCTGTACGGATTACAGAGGACAATCAACTGGTTCGCTTTCATT
TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	100	100	1
comGF/cglF	Streptococcus pneumoniae D39	100	100	1
comGF/cglF	Streptococcus pneumoniae R6	100	100	1
comGF/cglF	Streptococcus pneumoniae TIGR4	100	100	1
comGF/cglF	Streptococcus mitis SK321	90.85	100	0.909
comGF/cglF	Streptococcus mitis NCTC 12261	89.542	100	0.895