Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EL277_RS10570 Genome accession   NZ_AP018936
Coordinates   1973321..1974337 (-) Length   338 a.a.
NCBI ID   WP_074196785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain NU83127     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1968321..1979337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL277_RS12635 - 1968398..1968529 (-) 132 WP_000768904.1 hypothetical protein -
  EL277_RS10530 (SPNU_1989) - 1968530..1969720 (-) 1191 WP_000167757.1 acetate kinase -
  EL277_RS10535 (SPNU_1990) comYH 1969771..1970724 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  EL277_RS10540 - 1970785..1971379 (-) 595 Protein_1954 class I SAM-dependent methyltransferase -
  EL277_RS10545 (SPNU_1993) comGG/cglG 1971516..1971929 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  EL277_RS10550 (SPNU_1994) comGF/cglF 1971907..1972368 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL277_RS10555 (SPNU_1995) comGE/cglE 1972331..1972633 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EL277_RS10560 (SPNU_1996) comGD/cglD 1972596..1973000 (-) 405 WP_000588028.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL277_RS10565 (SPNU_1997) comGC/cglC 1972993..1973319 (-) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  EL277_RS10570 (SPNU_1998) comGB/cglB 1973321..1974337 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL277_RS10575 (SPNU_1999) comGA/cglA/cilD 1974285..1975226 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  EL277_RS10580 (SPNU_2000) - 1975302..1975667 (-) 366 WP_000286415.1 DUF1033 family protein -
  EL277_RS10585 (SPNU_2001) - 1975818..1976876 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  EL277_RS10590 (SPNU_2002) nagA 1977039..1978190 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38392.40 Da        Isoelectric Point: 9.4802

>NTDB_id=71826 EL277_RS10570 WP_074196785.1 1973321..1974337(-) (comGB/cglB) [Streptococcus pneumoniae strain NU83127]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=71826 EL277_RS10570 WP_074196785.1 1973321..1974337(-) (comGB/cglB) [Streptococcus pneumoniae strain NU83127]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

100

100

1
  comGB/cglB Streptococcus pneumoniae D39

100

100

1
  comGB/cglB Streptococcus pneumoniae R6

100

100

1
  comGB/cglB Streptococcus pneumoniae TIGR4

100

100

1
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.547

94.083

0.541
  comYB Streptococcus mutans UA159

57.547

94.083

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment