Detailed information    

experimental Experimentally validated

Overview

Name   ceuB   Type   Machinery gene
Locus tag   CJJ81176_RS06500 Genome accession   NC_008787
Coordinates   1268177..1269145 (+) Length   322 a.a.
NCBI ID   WP_002869322.1    Uniprot ID   -
Organism   Campylobacter jejuni subsp. jejuni 81-176     
Function   DNA uptake   
DNA binding and uptake

Function

Several of these mutants (ceuB::solo, ctsG::solo, proC::solo, and ctsT::solo) take up DNA at background levels, much like the strains with solo insertions in the operon ranging from ctsR to ctsF. In these mutants and in the type II secretion system mutants, we were unable to distinguish whether the transformation defect lies in transport of DNA across the outer membrane or if the defect is in the binding step of transformation, since the results from our DNA-binding assays were not consistent.


Genomic Context

Location: 1263177..1274145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJJ81176_RS06475 (CJJ81176_1346) cdsA 1264084..1264809 (-) 726 WP_002869324.1 phosphatidate cytidylyltransferase -
  CJJ81176_RS06480 (CJJ81176_1347) - 1264818..1265156 (-) 339 WP_002855905.1 hypothetical protein -
  CJJ81176_RS06485 (CJJ81176_1348) - 1265158..1266465 (-) 1308 WP_002856257.1 NFACT family protein -
  CJJ81176_RS06490 (CJJ81176_1349) mobA 1266527..1267102 (+) 576 WP_002855734.1 molybdenum cofactor guanylyltransferase -
  CJJ81176_RS06495 (CJJ81176_1350) pldA 1267099..1268088 (+) 990 WP_002855844.1 phospholipase A -
  CJJ81176_RS06500 (CJJ81176_1351) ceuB 1268177..1269145 (+) 969 WP_002869322.1 ABC transporter permease Machinery gene
  CJJ81176_RS06505 (CJJ81176_1352) ceuC 1269138..1270076 (+) 939 WP_002869321.1 iron chelate uptake ABC transporter family permease subunit -
  CJJ81176_RS06510 (CJJ81176_1353) ceuD 1270073..1270828 (+) 756 WP_002856278.1 ABC transporter ATP-binding protein -
  CJJ81176_RS06515 (CJJ81176_1354) ceuB 1270841..1271833 (+) 993 WP_011812790.1 enterochelin ABC transporter substrate-binding protein CeuB -
  CJJ81176_RS06520 (CJJ81176_1355) - 1271987..1272208 (+) 222 WP_002856036.1 hypothetical protein -
  CJJ81176_RS06525 (CJJ81176_1356) - 1272209..1272490 (+) 282 WP_009882907.1 type II toxin-antitoxin system RelE/ParE family toxin -
  CJJ81176_RS06535 (CJJ81176_1358) - 1272850..1273404 (-) 555 WP_002855904.1 membrane protein -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 35397.29 Da        Isoelectric Point: 9.6678

>NTDB_id=1235 CJJ81176_RS06500 WP_002869322.1 1268177..1269145(+) (ceuB) [Campylobacter jejuni subsp. jejuni 81-176]
MFFKHILSLKVLIALLLFFGMISLFIGVISINVKDILNLNSTQLEIITLTRIPRLIAILLTGMSLSICGLIMQQLTQNKF
VSPTTAGTMDCAKFGILISLIFFAGASFFTQAIIASVFALLGSFIFIQILRKIKLKDVIFVPLIGLMFGGIISAITTFFA
YALNYIQNIQGWLQGSMANVMQGNYELLYISLPLFILAYFLAHKITIVGMGEDIALNLGISYNGILFLGLMIVSIITSLV
IVSVGIIPFLGLIIPNLVALYLGDNLRKNLIYIALCGALFLLVCDIISRLVIFPFEMPLSITTGVLGSLIFIFLLLKRKA
YA

Nucleotide


Download         Length: 969 bp        

>NTDB_id=1235 CJJ81176_RS06500 WP_002869322.1 1268177..1269145(+) (ceuB) [Campylobacter jejuni subsp. jejuni 81-176]
TTGTTTTTTAAGCATATATTGAGTTTAAAAGTGCTTATAGCTTTACTTTTATTCTTTGGAATGATAAGTTTATTTATAGG
AGTTATCAGTATCAATGTAAAAGATATTCTTAATCTTAACTCCACTCAACTAGAAATCATAACCCTCACAAGAATTCCTA
GACTTATAGCAATTTTACTCACGGGAATGAGTCTTAGTATATGCGGGCTTATCATGCAACAACTCACGCAAAATAAATTC
GTTTCTCCAACTACGGCAGGGACTATGGATTGTGCTAAATTTGGCATACTTATTTCTTTAATATTTTTTGCTGGAGCGTC
TTTTTTCACCCAAGCTATCATTGCTTCTGTATTTGCACTTTTGGGTTCTTTTATATTTATCCAAATTTTAAGAAAAATCA
AACTCAAAGATGTGATTTTTGTACCTTTGATAGGCTTGATGTTTGGAGGCATTATTAGTGCTATAACTACTTTTTTTGCC
TATGCTTTAAATTATATACAAAATATCCAAGGCTGGTTACAAGGCAGTATGGCAAATGTTATGCAGGGAAATTATGAATT
GCTTTACATTTCTTTACCACTTTTTATACTTGCTTATTTTTTAGCTCATAAAATCACCATAGTTGGCATGGGCGAAGATA
TAGCTTTAAATCTTGGAATTTCTTATAATGGGATATTATTTTTAGGCTTGATGATTGTAAGCATTATCACTAGCTTAGTG
ATTGTAAGCGTTGGAATTATCCCTTTTTTAGGGCTAATTATCCCAAATTTAGTAGCTCTTTATCTAGGTGATAATCTTAG
AAAAAATCTTATCTATATAGCACTTTGTGGAGCTTTGTTTTTACTTGTTTGCGATATCATTTCAAGACTTGTCATCTTTC
CTTTTGAAATGCCTTTAAGTATCACTACAGGTGTTTTAGGATCTTTAATCTTTATCTTTCTTTTGCTAAAAAGGAAAGCT
TATGCGTAA

Domains

Predicted by InterproScan.

FecCD transport family (FecCD)

(15-317)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Rebecca S Wiesner et al. (2003) Natural transformation of Campylobacter jejuni requires components of a type II secretion system. Journal of Bacteriology 185(18):5408-18. [PMID: 12949093]