Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NUT31_RS08400 Genome accession   NZ_CP102454
Coordinates   1919014..1920153 (+) Length   379 a.a.
NCBI ID   WP_168967127.1    Uniprot ID   -
Organism   Aeromonas sp. BC14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1914014..1925153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUT31_RS08370 (NUT31_08365) - 1914321..1915385 (+) 1065 WP_011706545.1 3-deoxy-7-phosphoheptulonate synthase -
  NUT31_RS08375 (NUT31_08370) aac(6') 1915392..1915850 (+) 459 WP_268015252.1 aminoglycoside 6'-N-acetyltransferase -
  NUT31_RS08380 (NUT31_08375) - 1915872..1916657 (-) 786 WP_017410872.1 ATP-binding cassette domain-containing protein -
  NUT31_RS08385 (NUT31_08380) btuC 1916644..1917651 (-) 1008 WP_268015253.1 vitamin B12 ABC transporter permease BtuC -
  NUT31_RS08390 (NUT31_08385) - 1917752..1918483 (-) 732 WP_076361396.1 transporter substrate-binding domain-containing protein -
  NUT31_RS08395 (NUT31_08390) - 1918597..1918953 (+) 357 WP_005302630.1 DMT family protein -
  NUT31_RS08400 (NUT31_08395) pilU 1919014..1920153 (+) 1140 WP_168967127.1 type IVa pilus ATPase TapW Machinery gene
  NUT31_RS08405 (NUT31_08400) glgC 1920437..1921708 (+) 1272 WP_011706551.1 glucose-1-phosphate adenylyltransferase -
  NUT31_RS08410 (NUT31_08405) - 1921844..1922323 (+) 480 WP_268015254.1 WbuC family cupin fold metalloprotein -
  NUT31_RS08415 (NUT31_08410) - 1922424..1922666 (-) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  NUT31_RS08420 (NUT31_08415) feoB 1922663..1924936 (-) 2274 WP_268015255.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42304.39 Da        Isoelectric Point: 6.6010

>NTDB_id=717118 NUT31_RS08400 WP_168967127.1 1919014..1920153(+) (pilU) [Aeromonas sp. BC14]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDNLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=717118 NUT31_RS08400 WP_168967127.1 1919014..1920153(+) (pilU) [Aeromonas sp. BC14]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAATGAAACGCCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCTGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAGTCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACTCTGCGTCAGGCACCCGACGTCATCCTCATCGGTGAAATTCGCGACCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCGATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAACTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
GCTGGTTAAGACTACTGATGGCGGTCGCCGGGCGGCAGTGGAGATCATGCTGGGCACCCACACCATTCGCGACATGATCA
AACGTGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTAGAAGGTGTGATCGACGAGGAGGAGGCAGTAAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGACG
AAAAAGACAATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.947

100

0.541
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.988

90.765

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter nosocomialis M2

41.317

88.127

0.364
  pilT Acinetobacter baumannii strain A118

41.317

88.127

0.364
  pilT Acinetobacter baumannii D1279779

41.317

88.127

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364