Detailed information NTG0700867

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NF675_RS12025
• Genome accession: NZ_CP099596
• Coordinates: 2735226..2736335 (+)
• Length: 369 a.a.
• NCBI ID: WP_095179759.1
• Uniprot ID: A0AAQ3J945
• Organism: Pseudomonas siliginis strain OTU6BAGNBB1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2730226..2741335

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NF675_RS12010 (NF675_12010)	-	2731200..2732135 (+)	936	WP_150742013.1	sugar kinase	-
NF675_RS12015 (NF675_12015)	-	2732251..2732526 (-)	276	WP_252880016.1	peptidylprolyl isomerase	-
NF675_RS12020 (NF675_12020)	-	2732567..2735116 (-)	2550	WP_150742014.1	PAS domain-containing sensor histidine kinase	-
NF675_RS12025 (NF675_12025)	pilU	2735226..2736335 (+)	1110	WP_095179759.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NF675_RS12030 (NF675_12030)	-	2736565..2740167 (+)	3603	WP_252880017.1	hypothetical protein	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40886.84 Da
• Isoelectric Point: 6.9929

>NTDB_id=700867 NF675_RS12025 WP_095179759.1 2735226..2736335(+) (pilU) [Pseudomonas siliginis strain OTU6BAGNBB1]
MEIDALLSTLSQKNGSDLFLSTGAPPSARIDGVLTALSDQPFKPGETAAIATSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKRGLILFVGATSSGKSTSLAALIDHRNRHSAGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVLSTLHATNANQALDRI
INMFPEEKREQLLQTLGNNLKAFISQRLVRTVDGQRRAAVEVLLGTPTVADALRRNELAELKSIMEKSEDSGMQTFDAAL
LQLVLARVITVEEALKNADSTNNLRLGIKLKLDSPINEKASTGDWGLVE

Nucleotide

• Length: 1110 bp

>NTDB_id=700867 NF675_RS12025 WP_095179759.1 2735226..2736335(+) (pilU) [Pseudomonas siliginis strain OTU6BAGNBB1]
ATGGAAATCGATGCACTGTTGTCAACATTGTCGCAAAAAAACGGTTCGGATCTCTTCCTTTCCACCGGCGCACCACCGAG
CGCTCGCATTGATGGTGTGCTGACGGCGCTGAGCGATCAGCCATTCAAACCTGGCGAAACCGCGGCCATCGCCACCTCCC
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTTGAAATGAACCTGGCAATCTCCCGAACGGGCGTCGGCCGG
TTCCGGGTGAACATTTTCAAGCAGCGCAACGACGTTTCGATCGTTATCCGCAACGTCAAGCTCGACATCCCGCGGTTCGA
AGACTTGAAACTGCCGCCGGTGTTGCTGGAAACGGTGATGCTCAAACGTGGCCTGATTCTTTTTGTCGGCGCTACCAGTT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATCATCGCAACCGCCACAGTGCCGGACACATTGTTACCATCGAAGAC
CCGATCGAATATATCCATCGGCACAAGCGCTCGATCATCAATCAGCGTGAGGTTGGCGTCGATACGCGCAGCTTTCATGC
CGCGCTGAAGAACACTTTACGCCAGGCACCGGACGTCGTCCTGATTGGTGAGATCCGTGACCGCGAAACCATGGAGCACG
CGCTCTCGTTTGCCGATACAGGGCATCTTGTGTTGTCCACGTTACATGCGACCAATGCCAATCAGGCGCTGGATCGCATC
ATCAATATGTTTCCCGAGGAGAAGCGCGAGCAGCTGTTGCAGACATTGGGCAACAACCTGAAAGCCTTTATCTCGCAGCG
CTTGGTACGCACTGTCGACGGCCAGCGCAGGGCGGCCGTGGAAGTGTTATTAGGTACGCCGACTGTTGCTGATGCGCTCC
GTCGCAATGAGTTGGCTGAGTTGAAAAGTATCATGGAGAAATCCGAAGACTCAGGCATGCAGACATTCGATGCTGCACTG
TTACAGTTAGTCCTTGCGCGTGTTATTACAGTAGAAGAAGCTTTGAAAAACGCGGATTCGACAAACAACTTACGGTTAGG
CATCAAGCTTAAACTTGATTCTCCAATCAACGAAAAAGCATCGACAGGGGATTGGGGGTTGGTGGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.84	95.122	0.531
pilU	Acinetobacter baylyi ADP1	53.125	95.393	0.507
pilU	Vibrio cholerae strain A1552	51.42	95.393	0.491
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.298	91.87	0.379
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.541	90.244	0.366
pilT	Vibrio cholerae strain A1552	40.541	90.244	0.366
pilT	Neisseria meningitidis 8013	38.841	93.496	0.363
pilT	Neisseria gonorrhoeae MS11	38.617	94.038	0.363
pilT	Pseudomonas stutzeri DSM 10701	38.663	93.225	0.36
pilT	Pseudomonas aeruginosa PAK	38.663	93.225	0.36
pilT	Acinetobacter baumannii D1279779	39.701	90.786	0.36
pilT	Acinetobacter baumannii strain A118	39.701	90.786	0.36
pilT	Acinetobacter nosocomialis M2	39.701	90.786	0.36