Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M2I89_RS25515 Genome accession   NZ_CP096937
Coordinates   5383320..5383772 (-) Length   150 a.a.
NCBI ID   WP_028681203.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY5520     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5365254..5383043 5383320..5383772 flank 277


Gene organization within MGE regions


Location: 5365254..5383772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2I89_RS25410 (M2I89_25350) - 5365254..5365796 (-) 543 WP_123809552.1 hypothetical protein -
  M2I89_RS25415 (M2I89_25355) - 5365940..5366140 (+) 201 WP_023082007.1 helix-turn-helix domain-containing protein -
  M2I89_RS25420 (M2I89_25360) - 5366137..5366553 (+) 417 WP_034054964.1 hypothetical protein -
  M2I89_RS25425 (M2I89_25365) - 5366546..5366779 (+) 234 WP_031653969.1 hypothetical protein -
  M2I89_RS25430 (M2I89_25370) - 5366776..5367024 (+) 249 WP_031653970.1 hypothetical protein -
  M2I89_RS25435 (M2I89_25375) - 5367021..5367326 (+) 306 WP_023109345.1 hypothetical protein -
  M2I89_RS31925 - 5367329..5367766 (+) 438 WP_308416434.1 hypothetical protein -
  M2I89_RS25445 (M2I89_25385) - 5367805..5368272 (+) 468 WP_031629313.1 hypothetical protein -
  M2I89_RS25450 (M2I89_25390) - 5368279..5369232 (+) 954 WP_308416433.1 DUF3631 domain-containing protein -
  M2I89_RS25455 (M2I89_25395) - 5369232..5369525 (+) 294 WP_221345446.1 hypothetical protein -
  M2I89_RS25460 (M2I89_25400) - 5369581..5370477 (+) 897 WP_034069047.1 hypothetical protein -
  M2I89_RS25465 (M2I89_25405) - 5371183..5372358 (+) 1176 WP_223822176.1 DUF262 domain-containing protein -
  M2I89_RS25470 (M2I89_25410) - 5372369..5373196 (+) 828 WP_124157035.1 MAE_28990/MAE_18760 family HEPN-like nuclease -
  M2I89_RS25475 (M2I89_25415) - 5374510..5375016 (-) 507 WP_034064628.1 GNAT family N-acetyltransferase -
  M2I89_RS25480 (M2I89_25420) - 5375789..5376058 (+) 270 WP_225007575.1 hypothetical protein -
  M2I89_RS25485 (M2I89_25425) - 5376058..5376294 (+) 237 WP_003096116.1 hypothetical protein -
  M2I89_RS25490 (M2I89_25430) - 5376433..5376669 (-) 237 WP_071536299.1 hypothetical protein -
  M2I89_RS25495 (M2I89_25435) - 5376697..5379459 (-) 2763 WP_057379627.1 RHS repeat domain-containing protein -
  M2I89_RS25500 (M2I89_25440) - 5379474..5380622 (-) 1149 WP_172773772.1 DUF6531 domain-containing protein -
  M2I89_RS25505 (M2I89_25445) - 5382801..5383043 (+) 243 WP_023109353.1 hypothetical protein -
  M2I89_RS25515 (M2I89_25455) pilA 5383320..5383772 (-) 453 WP_028681203.1 pilin Machinery gene

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15671.99 Da        Isoelectric Point: 7.7813

>NTDB_id=685399 M2I89_RS25515 WP_028681203.1 5383320..5383772(-) (pilA) [Pseudomonas aeruginosa strain NY5520]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVDIKDTGDGTVKFTFATGQSSPKNAGTAITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=685399 M2I89_RS25515 WP_028681203.1 5383320..5383772(-) (pilA) [Pseudomonas aeruginosa strain NY5520]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACTACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGATATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGCAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

65.789

100

0.667
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45

100

0.48
  pilA Vibrio cholerae strain A1552

45

100

0.48
  pilA Vibrio cholerae C6706

45

100

0.48
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46

100

0.46
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.958

95.333

0.4
  pilA2 Legionella pneumophila strain ERS1305867

38.926

99.333

0.387
  pilA2 Legionella pneumophila str. Paris

38.255

99.333

0.38
  pilA Acinetobacter nosocomialis M2

42.537

89.333

0.38
  pilA Ralstonia pseudosolanacearum GMI1000

39.583

96

0.38