Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MZN68_RS02075 Genome accession   NZ_CP096117
Coordinates   420937..422067 (+) Length   376 a.a.
NCBI ID   WP_002214012.1    Uniprot ID   X5EQV8
Organism   Neisseria meningitidis strain DFS-1910310016     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415937..427067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MZN68_RS02055 (MZN68_02050) - 416498..416647 (+) 150 WP_019273745.1 hypothetical protein -
  MZN68_RS02060 (MZN68_02055) lepB 416866..417885 (-) 1020 WP_002214017.1 signal peptidase I -
  MZN68_RS02065 (MZN68_02060) lepA 418028..419821 (-) 1794 WP_002214015.1 translation elongation factor 4 -
  MZN68_RS02070 (MZN68_02065) - 420068..420769 (-) 702 WP_002235632.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  MZN68_RS02075 (MZN68_02070) pilU 420937..422067 (+) 1131 WP_002214012.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MZN68_RS02080 (MZN68_02075) - 422101..423078 (+) 978 WP_002239674.1 DNA polymerase III subunit delta' -
  MZN68_RS02085 (MZN68_02080) - 423082..423432 (+) 351 WP_002239593.1 PilZ domain-containing protein -
  MZN68_RS02090 (MZN68_02085) - 423437..424216 (+) 780 WP_002239604.1 TatD family hydrolase -
  MZN68_RS02095 - 424242..424456 (+) 215 Protein_410 DUF333 domain-containing protein -
  MZN68_RS02100 (MZN68_02100) grxD 424557..424868 (+) 312 WP_002239630.1 Grx4 family monothiol glutaredoxin -
  MZN68_RS02105 (MZN68_02105) upp 424974..425600 (+) 627 WP_002214002.1 uracil phosphoribosyltransferase -
  MZN68_RS02110 (MZN68_02110) - 425623..425943 (+) 321 WP_002214001.1 DUF2322 family protein -
  MZN68_RS02115 (MZN68_02115) - 426346..426768 (+) 423 WP_002213999.1 protein Gly1ORF1 -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.36 Da        Isoelectric Point: 6.4333

>NTDB_id=679491 MZN68_RS02075 WP_002214012.1 420937..422067(+) (pilU) [Neisseria meningitidis strain DFS-1910310016]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=679491 MZN68_RS02075 WP_002214012.1 420937..422067(+) (pilU) [Neisseria meningitidis strain DFS-1910310016]
ATGTTTACCGATGAAAATATGACCGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCGATGAAGTTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGCGCGACGGCGTTGGTATTCCG
CGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCTGTCTTGAAGGATGTCGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTTGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGACCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATCACCCAGCGCGA
GGTCGGCGTGGATACGGAAAACTGGATGGCGGCGTTGAAAAACACGCTGCGTCAGGCGCCGGATGTGATTCTGATCGGCG
AAATCCGCGACCGCGAAACAATGGACTACGCCATTGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCACTCGACCGCATCATCAACTTTTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGATATTTCCCTGCAAGAAGCATTGAA
AAATGCCGATTCCGCACACGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCCGCGCGCAAAGTTCCAGCCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB X5EQV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.683

87.234

0.372
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.768

87.234

0.364