Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THC_RS04205 Genome accession   NZ_AP014945
Coordinates   851466..852557 (-) Length   363 a.a.
NCBI ID   WP_068513782.1    Uniprot ID   A0A0U5AGV5
Organism   Caldimicrobium thiodismutans strain TF1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 846466..857557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THC_RS04185 (THC_0844) priA 846636..848978 (-) 2343 WP_068513770.1 primosomal protein N' -
  THC_RS04190 (THC_0845) - 848978..849850 (-) 873 WP_068513773.1 UTP--glucose-1-phosphate uridylyltransferase -
  THC_RS04195 (THC_0846) - 849847..850302 (-) 456 WP_068513775.1 hypothetical protein -
  THC_RS04200 (THC_0847) - 850299..851462 (-) 1164 WP_068513779.1 type IV pilus twitching motility protein PilT -
  THC_RS04205 (THC_0848) pilT 851466..852557 (-) 1092 WP_068513782.1 type IV pilus twitching motility protein PilT Machinery gene
  THC_RS04210 (THC_0849) - 852743..852976 (-) 234 WP_068513785.1 hypothetical protein -
  THC_RS04215 (THC_0850) queC 853417..854082 (+) 666 WP_068513788.1 7-cyano-7-deazaguanine synthase QueC -
  THC_RS04220 (THC_0851) - 854079..854447 (+) 369 WP_068513793.1 YkgJ family cysteine cluster protein -
  THC_RS04225 (THC_0852) - 854505..854924 (+) 420 WP_082706278.1 Fur family transcriptional regulator -
  THC_RS04230 (THC_0853) - 854936..855787 (+) 852 WP_068513796.1 NAD(P)-dependent oxidoreductase -
  THC_RS04240 (THC_0855) - 856397..857188 (+) 792 WP_068513800.1 Nif3-like dinuclear metal center hexameric protein -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40901.42 Da        Isoelectric Point: 6.5127

>NTDB_id=67509 THC_RS04205 WP_068513782.1 851466..852557(-) (pilT) [Caldimicrobium thiodismutans strain TF1]
MAKLDPFFKLMVDTGASDLHLSVGNPPLIRLHGDLQRIKYKNLNEDELREMLYEIAPEEIIKRFEEEGEVDFGLELPGLA
RFRVNYYKERRGIAAAFRIIPTKVKTVEELGLPQILNKLALLPKGLILVTGPTGSGKSTTLAAVIDYANRMRYDHIITIE
DPIEFVHESKNCLVNQRELGTHTKSFANALRAALREDPDIILVGEMRDRETIALAIEATLTGHVVFSTLHTISAAQTINR
IIDVFPADEQAQIRTTLSEAIRAVISQTMFKRVDRPGRIVATEILIATPAVRNLIREGKIHQIPSMIQTGKKYGMMSLDD
VIMDYLNRNIISPEEAFIKAIDKTRFINFVQNKEAIDFTELPG

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=67509 THC_RS04205 WP_068513782.1 851466..852557(-) (pilT) [Caldimicrobium thiodismutans strain TF1]
ATGGCCAAGCTGGATCCCTTTTTCAAATTAATGGTAGATACAGGGGCATCAGACCTTCACCTCTCTGTTGGTAATCCTCC
CCTTATCAGGCTCCATGGAGACCTTCAGAGGATTAAATATAAAAACTTAAATGAAGATGAACTAAGAGAGATGCTTTATG
AAATTGCCCCTGAAGAGATAATAAAGCGTTTTGAAGAAGAAGGAGAAGTTGATTTTGGTCTTGAATTACCTGGTCTTGCC
CGCTTCAGGGTAAATTATTATAAGGAGAGAAGAGGAATAGCTGCAGCCTTCAGAATTATTCCTACTAAAGTGAAAACTGT
AGAGGAATTAGGGCTTCCTCAAATTTTGAACAAACTTGCCCTACTACCTAAGGGCTTAATTCTCGTCACTGGTCCAACAG
GATCTGGAAAATCAACTACATTGGCTGCAGTAATTGATTATGCTAATAGAATGCGCTATGATCATATTATAACCATTGAA
GATCCCATAGAATTTGTGCATGAATCCAAAAATTGTCTTGTTAACCAGAGGGAACTTGGAACTCATACTAAAAGTTTTGC
AAATGCTCTAAGAGCTGCCCTTAGAGAAGATCCAGATATTATCCTTGTTGGAGAAATGAGAGATAGAGAGACGATTGCTT
TAGCTATTGAGGCCACCCTTACAGGACATGTGGTCTTTTCTACACTCCATACCATCTCAGCTGCTCAGACCATTAACCGT
ATCATTGATGTCTTTCCTGCGGATGAGCAGGCCCAGATTAGAACTACCCTCTCAGAAGCTATTCGTGCTGTGATTTCACA
GACCATGTTTAAGAGAGTTGATCGTCCAGGAAGAATTGTAGCTACAGAAATCCTTATTGCAACTCCTGCTGTAAGGAACC
TTATCCGGGAGGGTAAAATTCACCAGATTCCTTCCATGATTCAAACTGGAAAAAAATACGGCATGATGAGTTTAGATGAT
GTAATTATGGATTATCTAAATCGAAATATTATTAGTCCTGAGGAAGCCTTTATTAAGGCTATAGATAAAACACGATTTAT
TAATTTTGTCCAAAACAAAGAGGCTATTGATTTTACCGAGTTACCGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U5AGV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

57.055

89.807

0.512
  pilT Pseudomonas stutzeri DSM 10701

57.055

89.807

0.512
  pilT Neisseria meningitidis 8013

54.227

94.49

0.512
  pilT Neisseria gonorrhoeae MS11

53.936

94.49

0.51
  pilT Acinetobacter baumannii D1279779

54.762

92.562

0.507
  pilT Acinetobacter nosocomialis M2

54.762

92.562

0.507
  pilT Acinetobacter baumannii strain A118

54.762

92.562

0.507
  pilT Legionella pneumophila strain ERS1305867

54.925

92.287

0.507
  pilT Legionella pneumophila strain Lp02

54.925

92.287

0.507
  pilT Acinetobacter baylyi ADP1

57.009

88.43

0.504
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.064

90.634

0.463
  pilT Vibrio cholerae strain A1552

51.064

90.634

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.701

95.868

0.457

Multiple sequence alignment