Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MTO68_RS04585 Genome accession   NZ_CP094620
Coordinates   992168..993286 (-) Length   372 a.a.
NCBI ID   WP_004722736.1    Uniprot ID   A0A077KUG3
Organism   Acinetobacter sp. NyZ410     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 987168..998286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTO68_RS04560 (MTO68_04560) - 988977..989432 (+) 456 WP_004819397.1 bacteriohemerythrin -
  MTO68_RS04565 (MTO68_04565) - 989636..990718 (+) 1083 WP_244015204.1 hypothetical protein -
  MTO68_RS04570 (MTO68_04570) - 990735..991055 (+) 321 WP_244015206.1 RnfH family protein -
  MTO68_RS04575 (MTO68_04575) bamE 991140..991538 (-) 399 WP_004722739.1 outer membrane protein assembly factor BamE -
  MTO68_RS04580 (MTO68_04580) fur 991651..992088 (+) 438 WP_004722737.1 ferric iron uptake transcriptional regulator -
  MTO68_RS04585 (MTO68_04585) pilU 992168..993286 (-) 1119 WP_004722736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MTO68_RS04590 (MTO68_04590) pilT 993312..994349 (-) 1038 WP_004722735.1 type IV pilus twitching motility protein PilT Machinery gene
  MTO68_RS04595 (MTO68_04595) - 994794..995486 (+) 693 WP_004819403.1 YggS family pyridoxal phosphate-dependent enzyme -
  MTO68_RS04600 (MTO68_04600) - 995609..996442 (+) 834 WP_244015208.1 undecaprenyl-diphosphate phosphatase -
  MTO68_RS04605 (MTO68_04605) - 996647..997450 (+) 804 WP_244015210.1 thioesterase family protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41869.16 Da        Isoelectric Point: 6.2461

>NTDB_id=670226 MTO68_RS04585 WP_004722736.1 992168..993286(-) (pilU) [Acinetobacter sp. NyZ410]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLNGEIIMQLLNSIMSEKQRKEFAETRECNFAITNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNSNSKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=670226 MTO68_RS04585 WP_004722736.1 992168..993286(-) (pilU) [Acinetobacter sp. NyZ410]
ATGGACTTTAATGACTTGCTCAACATGATGATTGAGCAAAAAGCATCAGACTTATTTATTACCGCCGATGTTGAACCATC
AATGAAAATTAATGGTCAAATTGTTCCTGTGGTAAAAACCAAGTTAAACGGTGAAATTATTATGCAATTGCTTAACTCTA
TTATGAGTGAAAAGCAGCGTAAAGAATTTGCTGAGACCCGTGAATGTAACTTTGCCATTACCAATCGCGATAAAACTGCG
CGTTTTCGTGTCAGCGCTTTTCAACAACGTGATATGCCTGGAATGGTTTTGCGTCGTATTGAAACTCAAATTCCGACTAT
GGATGAGCTTAAATTACCGCCTATTCTTAAAGATCTCGCAATGACCAAACGTGGGATCATTATCTTTGTTGGTGCAACAG
GGACAGGTAAATCAACTTCGCTTGCATCCATCATTGGTTATCGCAACCAAAATTCTAAAGGTCATATCATCACCATTGAA
GACCCAATTGAATTTGTACACGAACATGCGGGATGTATTATTACCCAACGTGAAGTGGGAATTGATACAGATTCTTTTGA
AATTGCGCTTAAAAATACTTTGCGTCAAGCACCAGATGTAATCTTGATTGGTGAGATCCGTTCTCGTGAAACCATGGACT
ATGCCATCGCTTTTGCTGAAACAGGTCACTTAGTCTTTGCCACACTGCATGCCAACAACGCCAACCAAGCAATTGACCGG
ATTATCCATTTCTTTGAGTCTGACCGTCATAGCCAGTTGTTTATGGATCTATCACTTAACCTCAAGGCCATGGTTGCACA
ACAATTGATTCCAACACCAGACGGTAATTCTCGTCGTGCTGCAATTGAAATTCTCATCAACTCACCCTTAGTTTCAGACT
TAATCCGTAAAGGTGATGTACATGAAATTAAGGATTTGATGAAGCGCTCTCGTGAATTGGGTATGCAGACATTCGACCAA
GCTTTATTTGACCTCTATAAAGCAGGACAAATTACCTATAAAGACGCACTTAAACATGCGGACTCTCCAAATGATTTACG
TCTACAGATCAAATTGGATGAAGAAGGTGGTGCACAGCTACTCAATTCAAATAGCAAACTCACCTTTGATGGACAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A077KUG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

69.126

98.387

0.68
  pilU Vibrio cholerae strain A1552

54.595

99.462

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.353

93.011

0.403
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter baylyi ADP1

39.65

92.204

0.366
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Neisseria meningitidis 8013

39.412

91.398

0.36
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36