Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MRY16_RS04265 Genome accession   NZ_CP094445
Coordinates   896989..898107 (-) Length   372 a.a.
NCBI ID   WP_004913902.1    Uniprot ID   S7Y7Y7
Organism   Acinetobacter junii strain A2-1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 891989..903107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRY16_RS04235 (MRY16_04235) - 892662..893510 (+) 849 WP_005403076.1 class II glutamine amidotransferase -
  MRY16_RS04240 (MRY16_04240) - 893793..894248 (+) 456 WP_004913914.1 bacteriohemerythrin -
  MRY16_RS04245 (MRY16_04245) - 894487..895566 (+) 1080 WP_243484266.1 hypothetical protein -
  MRY16_RS04250 (MRY16_04250) - 895566..895889 (+) 324 WP_243484267.1 RnfH family protein -
  MRY16_RS04255 (MRY16_04255) bamE 895945..896343 (-) 399 WP_004913908.1 outer membrane protein assembly factor BamE -
  MRY16_RS04260 (MRY16_04260) fur 896455..896892 (+) 438 WP_004952725.1 ferric iron uptake transcriptional regulator -
  MRY16_RS04265 (MRY16_04265) pilU 896989..898107 (-) 1119 WP_004913902.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MRY16_RS04270 (MRY16_04270) pilT 898133..899170 (-) 1038 WP_004960020.1 type IV pilus twitching motility protein PilT Machinery gene
  MRY16_RS04275 (MRY16_04275) - 899298..899984 (+) 687 WP_243484269.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41716.88 Da        Isoelectric Point: 6.1368

>NTDB_id=669535 MRY16_RS04265 WP_004913902.1 896989..898107(-) (pilU) [Acinetobacter junii strain A2-1]
MDFNDLLNLMIEQSASDLFVTADVEPSMKINGQIVPVAKAKLSGEIVGQLLHSIMSDKQRKEFEDTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETVIPTMDELKLPAILKDLAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHAQLHMDLSLNLRAMVAQQLIPTPDGNSRRAAIEILINTPLLSDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALYDLYKAGEITYKDALKHADSPNDLRLTIKLSEEGADQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=669535 MRY16_RS04265 WP_004913902.1 896989..898107(-) (pilU) [Acinetobacter junii strain A2-1]
ATGGATTTTAATGATTTACTCAATCTAATGATTGAACAAAGTGCTTCGGATTTATTTGTTACCGCTGATGTTGAACCTTC
AATGAAAATCAACGGTCAGATTGTTCCTGTGGCTAAAGCAAAACTTTCAGGCGAGATTGTTGGTCAGTTATTACACTCAA
TCATGAGTGATAAACAACGTAAAGAATTCGAAGATACTCGCGAGTGTAACTTCGCAATTATGAACCGTGAGAAAACAGCG
CGTTTTCGTGTGAGCGCGTTCCAACAACGTGATATGCCAGGCATGGTATTACGTCGTATCGAAACGGTTATTCCAACGAT
GGATGAATTAAAACTACCTGCAATCCTCAAAGATTTAGCGATGACCAAACGTGGCATCATTATCTTTGTTGGTGCAACAG
GTACAGGTAAATCAACCTCGCTCGCGTCAATGATCGGATATCGTAATCAAAACTCTAAAGGTCATATCATCACCATTGAA
GACCCGATCGAGTTTGTACACCAACACGCGGGTTGTATTGTGACTCAACGCGAAGTCGGTATTGATACAGACTCTTTTGA
AGTAGCTTTGAAAAATACACTTCGTCAAGCACCCGATGTGATCTTGATTGGTGAGATTCGCTCTCGTGAAACAATGGACT
ATGCGATTGCTTTCGCAGAAACAGGTCACTTAGTACTTGCTACACTTCACGCGAATAACGCTAACCAAGCATTAGACCGT
ATTATTCACTTCTTTGAAGCTGACCGTCATGCTCAACTACATATGGATTTATCGCTCAATCTAAGAGCAATGGTTGCTCA
ACAGTTAATTCCAACACCTGATGGAAATTCGCGCCGAGCAGCTATCGAAATTCTAATTAATACACCATTACTATCAGATT
ATATCCGTAAAGGTGAAATTCATGAGATTAAAGACCTCATGAAACGCTCGCGCGAATTGGGCATGCAAACATTTGACCAA
GCGCTTTATGATTTATATAAAGCTGGTGAAATCACATATAAAGATGCGCTCAAACATGCAGACTCACCAAACGATTTACG
TCTCACCATTAAACTTTCTGAAGAAGGTGCAGATCAATTGTTAAGTGCAAGTCGAAACATTACATTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7Y7Y7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.132

100

0.763
  pilU Pseudomonas stutzeri DSM 10701

70.62

99.731

0.704
  pilU Vibrio cholerae strain A1552

55.135

99.462

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Pseudomonas aeruginosa PAK

39.884

93.011

0.371
  pilT Neisseria gonorrhoeae MS11

40.058

91.935

0.368
  pilT Neisseria meningitidis 8013

40.058

91.935

0.368
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Legionella pneumophila strain Lp02

40.476

90.323

0.366
  pilT Legionella pneumophila strain ERS1305867

40.476

90.323

0.366

Multiple sequence alignment