Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PBDP_RS25300 Genome accession   NZ_AP014703
Coordinates   5784049..5784459 (-) Length   136 a.a.
NCBI ID   WP_109756404.1    Uniprot ID   -
Organism   Pseudomonas sp. St290     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5779049..5789459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PBDP_RS25285 (PBDP_4995) - 5779886..5782819 (+) 2934 WP_212801465.1 DUF748 domain-containing protein -
  PBDP_RS25290 (PBDP_4996) - 5782820..5783116 (+) 297 WP_109756358.1 DUF2845 domain-containing protein -
  PBDP_RS25295 (PBDP_4997) - 5783428..5783781 (-) 354 WP_109756359.1 BON domain-containing protein -
  PBDP_RS25300 (PBDP_4998) pilA 5784049..5784459 (-) 411 WP_109756404.1 pilin Machinery gene
  PBDP_RS25305 (PBDP_4999) pilB 5784683..5786383 (+) 1701 WP_109756360.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PBDP_RS25310 (PBDP_5000) pilC 5786386..5787603 (+) 1218 WP_109756361.1 type II secretion system F family protein Machinery gene
  PBDP_RS25315 (PBDP_5001) pilD 5787605..5788474 (+) 870 WP_212801466.1 A24 family peptidase Machinery gene
  PBDP_RS25320 (PBDP_5002) coaE 5788474..5789097 (+) 624 WP_212801467.1 dephospho-CoA kinase -
  PBDP_RS25325 (PBDP_5003) yacG 5789094..5789300 (+) 207 WP_109756364.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13711.83 Da        Isoelectric Point: 8.1087

>NTDB_id=66848 PBDP_RS25300 WP_109756404.1 5784049..5784459(-) (pilA) [Pseudomonas sp. St290]
MNKQSGFTLIELLIVVAIIGILATFALPQYSKYQARAKATAGLGEISALKVPYEDMMNQGTSPTAANMNITTPTSNCAIA
VTGTASAGTGTIVCTLSNAPAAVAGKTITLSRDAAGAWTCASNAAKEFLPAGCPGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=66848 PBDP_RS25300 WP_109756404.1 5784049..5784459(-) (pilA) [Pseudomonas sp. St290]
ATGAATAAGCAGAGTGGCTTTACCTTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCGACCTTTGCGTT
GCCGCAATATTCCAAGTACCAGGCGCGAGCCAAGGCAACGGCCGGGCTGGGGGAAATCTCTGCGTTGAAGGTGCCGTACG
AGGACATGATGAATCAGGGTACTAGCCCGACAGCAGCCAACATGAACATCACGACGCCTACCAGCAACTGCGCAATCGCA
GTCACGGGTACTGCGTCGGCAGGTACGGGTACCATCGTTTGTACCCTCTCGAATGCTCCTGCTGCCGTAGCGGGTAAGAC
GATAACCTTAAGTCGTGATGCCGCCGGCGCCTGGACATGCGCCTCTAACGCTGCCAAGGAATTCCTGCCGGCTGGCTGCC
CTGGCGTCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.175

100

0.485
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.118

100

0.441
  pilA Ralstonia pseudosolanacearum GMI1000

37.255

100

0.419
  comP Acinetobacter baylyi ADP1

38

100

0.419
  pilA/pilA1 Eikenella corrodens VA1

37.415

100

0.404
  pilA Pseudomonas aeruginosa PAK

37.162

100

0.404
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.299

98.529

0.397
  pilA Vibrio cholerae strain A1552

38.298

100

0.397
  pilA Vibrio cholerae C6706

38.298

100

0.397
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.298

100

0.397
  pilA2 Legionella pneumophila str. Paris

37.241

100

0.397
  pilA2 Legionella pneumophila strain ERS1305867

36.552

100

0.39

Multiple sequence alignment