Detailed information NTG0664654

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MOQ58_RS16535
• Genome accession: NZ_CP093428
• Coordinates: 3631195..3632304 (-)
• Length: 369 a.a.
• NCBI ID: WP_280164025.1
• Uniprot ID: -
• Organism: Pseudomonas migulae strain 8R6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3626195..3637304

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MOQ58_RS16530 (MOQ58_16490)	-	3627207..3631055 (-)	3849	WP_280161392.1	hypothetical protein	-
MOQ58_RS16535 (MOQ58_16495)	pilU	3631195..3632304 (-)	1110	WP_280164025.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MOQ58_RS16540 (MOQ58_16500)	-	3632424..3634958 (+)	2535	WP_280161393.1	PAS domain-containing protein	-
MOQ58_RS16545 (MOQ58_16505)	-	3635055..3635330 (+)	276	WP_280161394.1	peptidylprolyl isomerase	-
MOQ58_RS16550 (MOQ58_16510)	-	3635470..3636402 (-)	933	WP_280161395.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41035.99 Da
• Isoelectric Point: 6.1928

>NTDB_id=664654 MOQ58_RS16535 WP_280164025.1 3631195..3632304(-) (pilU) [Pseudomonas migulae strain 8R6]
MEIDALLKTLSSQEGSDLYLSTGAPPCARFDGVLKPLAEQPFKPGEIAAIAASIMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSIVVRNIKLDIPRFEDLKLPPVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIVTIED
PIEYIHRHKKSIINQREVGVDTRSFHTALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPQERRMQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGSPTVGDLIRRNEFDELKGIMEKSAELGMQTFDGAL
YALVVEGVISEDEALKNADSLNNLKLHLKLHGEGASILSPSSDEWGLAD

Nucleotide

• Length: 1110 bp

>NTDB_id=664654 MOQ58_RS16535 WP_280164025.1 3631195..3632304(-) (pilU) [Pseudomonas migulae strain 8R6]
ATGGAAATCGATGCGCTGTTAAAGACCCTGTCCAGCCAGGAGGGTTCCGATTTGTACCTGTCCACCGGTGCGCCACCCTG
CGCCCGGTTTGACGGTGTACTCAAACCCTTGGCCGAGCAGCCGTTCAAACCGGGGGAAATCGCCGCGATTGCCGCGTCCA
TCATGGACGCCGAACAGCGATTGGAGTTCGATCGGGAACTGGAGATGAACCTGGCGATCTCGTTGGCGGGAGTAGGGCGT
TTTCGGGTCAATATTTTCAAGCAACGCAACGATGTGTCGATCGTGGTGCGCAACATCAAGCTCGACATTCCGCGTTTCGA
AGACCTCAAGTTGCCACCCGTGCTGCTGGAAACGGTGATGCTCAAACAAGGGCTGATGCTGTTCGTCGGCGCCACCGACT
CAGGCAAGTCGACCTCGCTGGCGGCGTTGATCGATTACCGCAACCGCCATAGCAGCGGGCATATCGTCACCATCGAAGAC
CCCATCGAGTACATCCACCGGCACAAAAAATCGATCATCAATCAGCGCGAGGTCGGCGTCGATACCCGCAGCTTTCATAC
CGCGCTGAAAAACACCCTGCGCCAGGCACCCGACGTGGTGCTGATCGGTGAGATCCGCGACCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGGCATTTGGTGATTTCCACCCTGCATGCTCACAACGCCCATCAGGCGCTGGATCGCATT
ATCAATTTCTTCCCGCAAGAACGGCGGATGCAACTGCTGCATGACCTGGGCAACAACCTTAAAGCGTTCGTTTCCCAACG
TCTGGTGCGCACTCGTGACGGTCAGCGCAGGGCGGCGGTAGAAGTGATGCTGGGATCGCCCACCGTTGGCGACTTGATAC
GGCGCAATGAGTTCGACGAGCTCAAAGGCATCATGGAAAAGTCCGCCGAATTGGGGATGCAGACCTTCGATGGCGCCTTG
TATGCGCTGGTGGTGGAAGGTGTGATCAGCGAGGATGAAGCGTTAAAAAATGCGGATTCGCTGAACAATTTAAAGTTGCA
CCTTAAGTTGCACGGGGAAGGAGCTTCGATATTAAGTCCGTCCAGTGATGAGTGGGGGTTGGCCGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.65	95.935	0.534
pilU	Acinetobacter baylyi ADP1	51.685	96.477	0.499
pilU	Vibrio cholerae strain A1552	51.714	94.851	0.491
pilT	Pseudomonas stutzeri DSM 10701	40.116	93.225	0.374
pilT	Pseudomonas aeruginosa PAK	39.826	93.225	0.371
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	90.244	0.371
pilT	Vibrio cholerae strain A1552	41.141	90.244	0.371
pilT	Legionella pneumophila strain Lp02	40.419	90.515	0.366
pilT	Legionella pneumophila strain ERS1305867	40.419	90.515	0.366
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.528	91.87	0.363
pilT	Acinetobacter baumannii D1279779	39.82	90.515	0.36
pilT	Acinetobacter baumannii strain A118	39.82	90.515	0.36
pilT	Acinetobacter nosocomialis M2	39.82	90.515	0.36