Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MOO40_RS08260 Genome accession   NZ_CP093308
Coordinates   1756783..1757922 (-) Length   379 a.a.
NCBI ID   WP_139392833.1    Uniprot ID   A0AAD3YMJ9
Organism   Aeromonas hydrophila strain AC185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1751783..1762922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOO40_RS08240 (MOO40_08265) feoB 1751982..1754255 (+) 2274 WP_241872124.1 Fe(2+) transporter permease subunit FeoB -
  MOO40_RS08245 (MOO40_08270) - 1754252..1754494 (+) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  MOO40_RS08250 (MOO40_08275) - 1754614..1755093 (-) 480 WP_162518464.1 WbuC family cupin fold metalloprotein -
  MOO40_RS08255 (MOO40_08280) glgC 1755229..1756500 (-) 1272 WP_241872125.1 glucose-1-phosphate adenylyltransferase -
  MOO40_RS08260 (MOO40_08285) pilU 1756783..1757922 (-) 1140 WP_139392833.1 type IVa pilus ATPase TapW Machinery gene
  MOO40_RS08265 (MOO40_08290) - 1757983..1758339 (-) 357 WP_005302630.1 DMT family protein -
  MOO40_RS08270 (MOO40_08295) - 1758453..1759184 (+) 732 WP_029300033.1 ABC transporter substrate-binding protein -
  MOO40_RS08275 (MOO40_08300) btuC 1759285..1760292 (+) 1008 WP_241872126.1 vitamin B12 ABC transporter permease BtuC -
  MOO40_RS08280 (MOO40_08305) - 1760279..1761064 (+) 786 WP_241872127.1 ATP-binding cassette domain-containing protein -
  MOO40_RS08285 (MOO40_08310) aac(6') 1761086..1761544 (-) 459 WP_241872128.1 aminoglycoside 6'-N-acetyltransferase -
  MOO40_RS08290 (MOO40_08315) - 1761551..1762615 (-) 1065 WP_029303894.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42278.35 Da        Isoelectric Point: 6.4290

>NTDB_id=663401 MOO40_RS08260 WP_139392833.1 1756783..1757922(-) (pilU) [Aeromonas hydrophila strain AC185]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=663401 MOO40_RS08260 WP_139392833.1 1756783..1757922(-) (pilU) [Aeromonas hydrophila strain AC185]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAGTGAGACGCCGCTCGAGCCTGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCTCCCGTGCTGCTCGACACCATCATGGAAAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAATCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGTCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
TGCCCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTAATCGGTGAAATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCAATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAGACTACCGATGGTGGACGCCGGGCGGCAGTAGAGATCATGCTGGGTACCCACACCATTCGCGACATGATCA
AACGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTAGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGACG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361