Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MIA32_RS08285 Genome accession   NZ_CP092356
Coordinates   1771667..1772806 (-) Length   379 a.a.
NCBI ID   WP_011706550.1    Uniprot ID   A0AAI9Q2R8
Organism   Aeromonas hydrophila strain CCL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1766667..1777806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIA32_RS08265 (MIA32_08250) feoB 1766875..1769148 (+) 2274 WP_016351201.1 Fe(2+) transporter permease subunit FeoB -
  MIA32_RS08270 (MIA32_08255) - 1769145..1769387 (+) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  MIA32_RS08275 (MIA32_08260) - 1769497..1769976 (-) 480 WP_016351200.1 WbuC family cupin fold metalloprotein -
  MIA32_RS08280 (MIA32_08265) glgC 1770112..1771383 (-) 1272 WP_011706551.1 glucose-1-phosphate adenylyltransferase -
  MIA32_RS08285 (MIA32_08270) pilU 1771667..1772806 (-) 1140 WP_011706550.1 type IVa pilus ATPase TapW Machinery gene
  MIA32_RS08290 (MIA32_08275) - 1772867..1773223 (-) 357 WP_005302630.1 DMT family protein -
  MIA32_RS08295 (MIA32_08280) - 1773337..1774068 (+) 732 WP_043119150.1 ABC transporter substrate-binding protein -
  MIA32_RS08300 (MIA32_08285) btuC 1774169..1775176 (+) 1008 WP_016351197.1 vitamin B12 ABC transporter permease BtuC -
  MIA32_RS08305 (MIA32_08290) - 1775163..1775948 (+) 786 WP_016351196.1 ATP-binding cassette domain-containing protein -
  MIA32_RS08310 (MIA32_08295) aac(6') 1775970..1776428 (-) 459 WP_016351195.1 aminoglycoside 6'-N-acetyltransferase -
  MIA32_RS08315 (MIA32_08300) - 1776435..1777499 (-) 1065 WP_016351194.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42264.32 Da        Isoelectric Point: 6.4290

>NTDB_id=656648 MIA32_RS08285 WP_011706550.1 1771667..1772806(-) (pilU) [Aeromonas hydrophila strain CCL1]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTSDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=656648 MIA32_RS08285 WP_011706550.1 1771667..1772806(-) (pilU) [Aeromonas hydrophila strain CCL1]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAATGGCGGCCTGCGTCCGCTCAGTGAAACGCCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGTCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAAATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAATCGACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATTGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
TGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGCGAGATCCGCGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCGATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAACTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
GCTGGTCAAAACCAGCGACGGTGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATTCGCGACATGATAA
AACGCGGTGAGTTCGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCGAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGACG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361