Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   MIH19_RS04945 Genome accession   NZ_CP092289
Coordinates   1039286..1039720 (-) Length   144 a.a.
NCBI ID   WP_283164098.1    Uniprot ID   -
Organism   Marinobacter sp. M1C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1030151..1052171 1039286..1039720 within 0


Gene organization within MGE regions


Location: 1030151..1052171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIH19_RS04905 (MIH19_04905) - 1030151..1030585 (-) 435 WP_249007810.1 adenylyltransferase/cytidyltransferase family protein -
  MIH19_RS04910 (MIH19_04910) - 1030578..1032923 (-) 2346 WP_249007811.1 glycosyltransferase -
  MIH19_RS04915 (MIH19_04915) - 1033128..1033940 (-) 813 WP_249007812.1 methionine biosynthesis protein MetW -
  MIH19_RS04920 (MIH19_04920) - 1033937..1035664 (-) 1728 WP_249007813.1 ABC transporter ATP-binding protein -
  MIH19_RS04925 (MIH19_04925) - 1035734..1036582 (-) 849 WP_249007814.1 glycosyltransferase family 2 protein -
  MIH19_RS04930 (MIH19_04930) - 1036603..1038573 (-) 1971 WP_249007815.1 hypothetical protein -
  MIH19_RS04935 (MIH19_04935) - 1038623..1039051 (-) 429 WP_283164097.1 pilin -
  MIH19_RS04945 (MIH19_04945) pilE 1039286..1039720 (-) 435 WP_283164098.1 pilin Machinery gene
  MIH19_RS04955 (MIH19_04955) - 1040088..1040324 (-) 237 WP_249007816.1 hypothetical protein -
  MIH19_RS04960 (MIH19_04960) - 1040577..1041008 (-) 432 WP_249007817.1 type II toxin-antitoxin system HicB family antitoxin -
  MIH19_RS04965 (MIH19_04965) - 1041049..1041231 (-) 183 WP_249007818.1 type II toxin-antitoxin system HicA family toxin -
  MIH19_RS04970 (MIH19_04970) - 1041336..1041878 (-) 543 WP_249007819.1 hypothetical protein -
  MIH19_RS04975 (MIH19_04975) - 1042013..1042279 (+) 267 WP_249007820.1 YlcI/YnfO family protein -
  MIH19_RS04980 (MIH19_04980) - 1042276..1042575 (+) 300 WP_249007821.1 type II toxin-antitoxin system RelE/ParE family toxin -
  MIH19_RS22515 - 1042812..1042928 (+) 117 WP_349292920.1 hypothetical protein -
  MIH19_RS04990 (MIH19_04990) - 1042930..1043232 (+) 303 WP_249007822.1 helix-turn-helix domain-containing protein -
  MIH19_RS04995 (MIH19_04995) - 1043350..1043730 (+) 381 WP_249007823.1 helix-turn-helix transcriptional regulator -
  MIH19_RS05000 (MIH19_05000) - 1043762..1043881 (-) 120 Protein_974 type II toxin-antitoxin system RelE/ParE family toxin -
  MIH19_RS05005 (MIH19_05005) - 1044024..1044212 (+) 189 Protein_975 ATP-binding protein -
  MIH19_RS05010 (MIH19_05010) bamE 1044322..1044795 (-) 474 WP_249007824.1 outer membrane protein assembly factor BamE -
  MIH19_RS05015 (MIH19_05015) - 1045059..1045331 (-) 273 WP_249007825.1 type II toxin-antitoxin system YafQ family toxin -
  MIH19_RS05020 (MIH19_05020) - 1045331..1045588 (-) 258 WP_249007826.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
  MIH19_RS05025 (MIH19_05025) - 1045940..1046434 (-) 495 WP_249007827.1 MgtC/SapB family protein -
  MIH19_RS22405 - 1046494..1046616 (+) 123 WP_283164099.1 hypothetical protein -
  MIH19_RS05030 (MIH19_05030) - 1046785..1047300 (+) 516 WP_249007828.1 sn-glycerol-3-phosphate transporter -
  MIH19_RS05035 (MIH19_05035) - 1047610..1047696 (-) 87 WP_249008946.1 type II toxin-antitoxin system HicB family antitoxin -
  MIH19_RS05040 (MIH19_05040) - 1047798..1047929 (-) 132 WP_249007829.1 type II toxin-antitoxin system HicA family toxin -
  MIH19_RS05045 (MIH19_05045) - 1048209..1048559 (+) 351 WP_249007830.1 DUF2784 domain-containing protein -
  MIH19_RS05050 (MIH19_05050) - 1048746..1049003 (+) 258 WP_249007831.1 helix-turn-helix transcriptional regulator -
  MIH19_RS05055 (MIH19_05055) - 1049035..1049325 (-) 291 WP_249007832.1 type II toxin-antitoxin system RelE/ParE family toxin -
  MIH19_RS05060 (MIH19_05060) - 1049315..1049563 (-) 249 WP_249007833.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  MIH19_RS05065 (MIH19_05065) - 1049742..1049996 (-) 255 WP_249008947.1 type II toxin-antitoxin system RelE/ParE family toxin -
  MIH19_RS05070 (MIH19_05070) - 1050023..1050295 (-) 273 WP_249007834.1 CopG family ribbon-helix-helix protein -
  MIH19_RS05075 (MIH19_05075) - 1050456..1051040 (-) 585 WP_249007835.1 histidine phosphatase family protein -
  MIH19_RS05080 (MIH19_05080) - 1051073..1051279 (-) 207 WP_249007836.1 hypothetical protein -
  MIH19_RS05085 (MIH19_05085) - 1051428..1052171 (-) 744 WP_249007837.1 ParA family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15031.03 Da        Isoelectric Point: 4.1546

>NTDB_id=656566 MIH19_RS04945 WP_283164098.1 1039286..1039720(-) (pilE) [Marinobacter sp. M1C]
MNKIQQGFTLIELMIVVAIIGILAAIAIPAYQDYTIRAQVSEGLVLASGAKTALAEFYNSTGRFPGDNDSLGLQAPTSIT
GSYVSGVDAGTDPGVIEVTFGVGVNEQIDGSTLEISAVTSAGSIRWTCREGTDLPGKYLPTNCR

Nucleotide


Download         Length: 435 bp        

>NTDB_id=656566 MIH19_RS04945 WP_283164098.1 1039286..1039720(-) (pilE) [Marinobacter sp. M1C]
ATGAATAAAATTCAACAGGGTTTTACACTTATCGAACTGATGATCGTGGTAGCGATCATCGGTATTCTGGCAGCCATCGC
AATTCCGGCGTATCAGGATTACACCATTCGTGCGCAGGTTTCCGAAGGTTTGGTTCTGGCTTCTGGTGCCAAGACTGCCT
TGGCCGAATTTTACAACAGCACTGGGCGCTTCCCAGGCGATAACGACTCTCTTGGGCTGCAGGCACCCACAAGTATCACG
GGTTCTTACGTGTCCGGTGTAGATGCTGGCACCGACCCCGGTGTGATTGAGGTCACGTTTGGCGTAGGCGTTAACGAACA
GATTGACGGGTCGACTCTCGAAATCTCCGCAGTAACTTCTGCAGGCAGTATTAGATGGACTTGCCGGGAAGGTACGGACC
TTCCTGGCAAGTACCTACCTACTAACTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

44.72

100

0.5
  pilE Neisseria gonorrhoeae strain FA1090

44.375

100

0.493
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.979

100

0.472
  pilA Ralstonia pseudosolanacearum GMI1000

41.212

100

0.472
  pilA2 Legionella pneumophila strain ERS1305867

46.429

97.222

0.451
  pilA2 Legionella pneumophila str. Paris

46.429

97.222

0.451
  pilA/pilA1 Eikenella corrodens VA1

40.645

100

0.437
  pilA Acinetobacter nosocomialis M2

43.478

95.833

0.417
  comP Acinetobacter baylyi ADP1

39.865

100

0.41
  pilA Acinetobacter baumannii strain A118

41.135

97.917

0.403
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.437

98.611

0.389
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.621

100

0.389
  pilA Pseudomonas aeruginosa PAK

34.437

100

0.361